Ion Torrent™ Next-Generation Sequencing Run Sequence
JUST ONE DAY
Ion Torrent™ technology delivers the fastest run times, at the most affordable price, of any next-generation sequencer.
Ion PGM™ System Workflow
The combination of Ion AmpliSeq™ target selection technology, the Ion PGM™ System, and fully automated template preparation from the upcoming Ion Chef™ System* offers the simplest and fastest* workflow for gene panel sequencing. Get meaningful results in just half the time of the alternative, with highly multiplexed Ion AmpliSeq™ DNA panels, the walk-away solution for preparing sequence-ready chips from libraries provided by the Ion Chef™ System, and faster sequencing on the Ion PGM™ System. From sample to results, there are no centrifugation steps, and half the pipetting steps and hands-on time of the alternative NGS workflow.
Ion PGM™ Applications
Targeted DNA Sequencing
Ion AmpliSeq™ technology transforms this application by enabling researchers to rapidly and simply amplify thousands of targets using just 10 ng of DNA. This technology, coupled with the Ion PGM™ System and easy-to-use Ion Reporter™ Software, enables researchers to go from precious FFPE samples to variants in a single day.
Targeted RNA Sequencing
Targeted RNA sequencing, using Ion AmpliSeq™ technology, is a sequencing-based gene expression profiling method that allows quantification of messenger RNA (mRNA) or targeted non-coding RNA (ncRNA) levels for up to 300 customer-defined genes in a single amplification reaction while using very low input (RNA - 500 pg of unfixed RNA or 5 ng of RNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples).
Rapid resequencing of mitochondrial genomes for disease research and biodiversity assessment.
The evolution of Ion semiconductor sequencing has enabled researchers to take advantage of increased throughput, higher accuracy, and longer reads to produce rapid and accurate sequencing of microbes with streamlined sample preparation and a simple and optimized data analysis workflow.
Chromatin Immunoprecipitation Sequencing (ChIP-Seq)
Assessing protein-DNA interactions at genome-wide level is essential for understanding a large number of biological processes during normal development and disease. Isolation of protein-DNA interactions by chromatin immunoprecipitation (ChIP) followed by massively parallel DNA sequencing is an unbiased method for identifying and quantitating DNA sites that are associated to a protein(s) of interest.
Ion Proton® System Workflow
Ion Torrent’s highly anticipated Ion Proton® System makes exome, transcriptome, and human-scale genome sequencing more accessible and affordable than ever.
Workflows for the Ion Proton® System include optimized library kits, automated template preparation, fast sequencing runs, and integrated data analysis solutions.
Ion Proton® Applications
The Ion Proton® System, combined with Ambion® RNA kits, delivers fast, flexible, and high-quality transcriptome sequencing at an affordable price.
The Ion Proton® System, combined with the Ion PII™ Chip, will make human-scale genome sequencing accessible with a benchtop sequencer affordable to most laboratory budgets. In addition, Ion Torrent™ next-generation semiconductor sequencing has enabled fast and accurate characterization of micro organisms; including bacteria, viruses, fungi & algae.
The Ion Proton® System, combined with Ion TargetSeq™ technology and Ion Reporter™ Software, delivers fast, flexible, and high-quality exome sequencing that scales with your research needs. Go from exome library to variant identification in a single day.
Chromatin Immunoprecipitation Sequencing
The Ion Proton™ System combined with the MAGnify™ Chromatin Immunoprecipitation System delivers fast, reliable, and high-quality ChIP-Seq results at an affordable price.
* The content provided herein may relate to products that have not been officially released and is subject to change without notice.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.