Ion Torrent Next-Generation Sequencing Select Targets
Transform your research with ready-to-use, custom, and community panels.
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- 10 ng of DNA per pool
- PCR-based target selection
- Up to 6,144 primers per pool
- One to hundreds of genes
- 96 barcodes for multiplexing
- Single day from DNA to results
- 2 hours to design custom panels
- 3.5 hours for target selection & library preparation
The most comprehensive gene coverage with the least amount of DNA
Ion AmpliSeq™ technology delivers simple and fast library construction for affordable targeted sequencing of specific human genes or genomic regions. Based on ultrahigh-multiplex PCR, Ion AmpliSeq™ technology requires as little as 10 ng of input DNA to target sets of genes, making sequencing of FFPE samples routine on Ion PGM™ Systems.
Alternative target selection methods are lengthy and complex and require large amounts of DNA, making them impractical for sequencing DNA from the formalin-fixed, paraffin-embedded (FFPE) tissues that are commonly used in translational and clinical research. Using just 10 ng of FFPE DNA and one pool of 6,144 primer pairs, variants can be identified in a single day using the Ion AmpliSeq™ Custom workflow. By contrast, the same project using alternative workflows requires 100 times as much DNA, 8 times the pools, and twice the time.
Ion AmpliSeq™ Single Day Workflow
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.