Targeted, quantitative
gene expression analysis


Ion AmpliSeq™ technology delivers simple and fast library construction, for affordable targeted sequencing of specific human genes. Ion AmpliSeq™ RNA Panels enable scientists to choose from over 20,000 targeted genes to survey gene expression fold changes in samples requiring as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA. The Ion PGM™ System, coupled with Ion AmpliSeq™ RNA Panels, Torrent Suite™ Software and Partek Flow® Software, enable a unique and complete workflow solution.

Ready-to-use Ion AmpliSeq™ RNA Panels
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Custom Ion AmpliSeq™ RNA Panels
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Ion AmpliSeq™ RNA workflowView now


 

 

Confirm expression using TaqMan® Assays

Universal Human Reference (UHR) RNA and Human Brain Reference (HBR) RNA control samples were used to compare fold-change for each of the 50 genes included in the Ion AmpliSeq™ RNA Cancer Panel against the fold change calculations of the same genes with TaqMan® Gene Expression Assays, resulting in a correlation of 0.989, as shown in Figure 1. These results show the accuracy of the gene expression data obtained with the Ion AmpliSeq™ RNA workflow. Find TaqMan® Assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons in the Ion Community.

Simple workflow

The 1.5 day workflow from RNA to annotated genes produces targeted libraries in 5.5 hours, after which template is prepared using the Ion One Touch™ 2 System and then sequenced on the Ion PGM™ Sequencer. Automated analysis is then performed with Torrent Suite™ Software, while additional analysis can be performed using Partek Flow® software.

Figure 2. Ion AmpliSeq™ RNA Cancer Panel workflow using an Ion 318™ Chip and 4.5 hour, 1 x 200 base sequencing run.


Ion AmpliSeq™ RNA Cancer Panel

The Ion AmpliSeq™ RNA Cancer Panel was developed as a RNA complement to the Ion AmpliSeq™ Cancer Hotspot Panel v2. The panel is a single pool of primers targeting 50 oncogenes and tumor suppressor genes including KRAS, BRAF, and EGFR genes.


Ion AmpliSeq™ RNA Apoptosis Panel

The Ion AmpliSeq™ Apoptosis Panel is a screening tool designed against 267 genes involved in the cellular apoptosis pathway. Included are genes associated with death receptor– mediated apoptosis, c-myc, and p53-mediated apoptosis.

 

Custom AmpliSeq™ RNA Panels

Ion AmpliSeq™ Designer is a free online tool that allows researchers to create and order Ion AmpliSeq™ RNA Custom Panels comprising just their genes of interest. Leveraging more than a decade of expertise powering the TaqMan® Custom Assay design pipeline, Ion AmpliSeq™ Designer produces optimized primer designs in just hours. You now have full flexibility to analyze hundreds of genes of your choice, such as those implicated in a particular disease state or representing specific biochemical pathways.

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Ordering Information for Ion Ampliseq™ Products

Catalog # Name Size List Price (USD) Qty
4482571 Ion AmpliSeq™ RNA Apoptosis Panel 24 reactions 600.00
4482572 Ion AmpliSeq™ RNA Cancer Panel 24 reactions 600.00
4482335 Ion AmpliSeq™ RNA Library Kit 8 reactions 880.00
4482340 Ion AmpliSeq™ RNA Library Kit 96 reactions 8,450.00
4482752 Ion AmpliSeq™ RNA Library Kit 384 reactions 22,280.00
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