Minutes to design Human
or Mouse DNA panels


Simplicity Scalability Speed
Customize with your genes 12 to 6,144 amplicons per pool Just minutes to submit design
10 ng of input DNA One to hundreds of genes Automated primer design in 2 hours
Simple PCR-based target selection 96 barcodes for multiplexing Targeted library constructed in 3.5 hours

 

If the Ion AmpliSeq™ Ready-to-use Panels don’t match your gene targets then simply select a subset of the targets or simply enter your specific genomic content online and we will design and create customized Ion AmpliSeq™ Panels just for you.

Creating and ordering custom panels is easy with the Ion AmpliSeq™ Designer—a free, online assay design tool that connects you directly to our assay design pipeline.  It's developed, optimized, and proven for human gene sequencing. All you need is a list of HUGO gene symbols or gene regions based on hg19 coordinates. Register at the Ion Community to use this solution.

Custom panels are delivered in two formats:  tubes of pre-pooled multiplexed primers in ready-to-use concentration and 384-well plates in which each well contains individual primer pairs in concentrated format.  For custom panels with ≤96 primer pairs, you will receive 2,250 total reactions.  For custom panels with >96 primer pairs, you will receive 9,000 total reactions.

 

Ion AmpliSeq™ Designer Performance Specifications

Specifications* Current value
Input DNA amount per primer pool 10 ng
Primer Pairs per pool 12 to 6,144
Target size 1 kb to 5 Mb
Target design rate >90%
Coverage uniformity >85%

*Performance values will vary with each custom design
Target design rate—bases covered by the insert region of amplicons out total target bases submitted
Typical coverage uniformity—bases covered at > 20% of the mean coverage

 

Ordering Information for Ion Ampliseq™ Products

Sku Name Size Price Qty
4475346 Ion AmpliSeq™ Cancer Hotspot Panel v2 8 reactions USD 200.00
4477686 Ion AmpliSeq™ Inherited Disease Panel 8 reactions USD 600.00
4477685 Ion AmpliSeq™ Comprehensive Cancer Panel 8 reactions USD 800.00
4471262 Ion AmpliSeq™ Cancer Panel Primer Pool 10 reactions USD 250.00
4475345 Ion AmpliSeq™ Library Kit 2.0 8 reactions USD 800.00
4479790 Ion AmpliSeq™ Sample ID Panel 96 reactions USD 125.00

For research use only. Not intended for any animal or human therapeutic or diagnostic use.