Simple, reproducible automated
sequencing

The Ion Chef™ System simplifies the workflow for Ion PGM™ and Ion Proton™ systems, providing an automated, walk-away workflow solution for reproducible template preparation and chip loading. And, coming soon, this capability will be extended to Ion AmpliSeq™ library preparation.

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Simplified workflow helps save time and labor

  • Intuitive set-up and instrument interactivity prior to template preparation and chip loading automation
  • Increased lab productivity—just 15 minutes of set-up time

Reduced run variability

  • Reduced user intervention enables reproducible chip loading for users of any experience level
  • Sample and reagent tracking with onboard tracking system

Coming soon—Ion AmpliSeq library preparation automation

  • Expanded Ion Chef capabilities enable automation of Ion AmpliSeq library generation
  • Accommodates one- or two-pool Ion AmpliSeq panels for DNA analysis
Preview: Ion AmpliSeq library preparation automation

Free up your time for higher value activities through the automation of your Ion Torrent™ Ion AmpliSeq™ targeted sequencing workflow with the Ion Torrent™ Ion Chef™ System.  Twenty minutes of upfront hands-on time using pre-packaged ready-to-go library preparation reagents enables walk-away convenience—return 7 hours later to take equalized and pooled libraries onto the template preparation step. Automated workflow supports Ion AmpliSeq one- and two-pool designs.

Dr. Mark Andersen from Thermo Fisher R&D discusses the capabilities and benefits of using the Ion Chef System for Ion AmpliSeq automated library preparation. View now

See how easy it is to automate Ion AmpliSeq library preparation on the Ion Chef System. View now

1 Preparation


Ion Chef

  • Load reagents & consumables
  • Set run parameters
  • Load up to 8 DNA samples & AmpliSeq panel
2 Automated Ion AmpliSeq library preparation
Ion Chef System

  • Targeted PCR amplification
  • Digest primers
  • Litigate library adaptors
  • Equalize & pool libraries

3 Preparation


Ion Chef

  • Load reagents & consumables
  • Load library sapmles
  • Set run parameters

4 Automated template prep & chip loading
Ion Chef System

  • Library amplification
  • ISP recovery & enrichment
  • Chip loading
Automated template preparation & chip loading with the Ion Chef System

The Ion Chef System simplifies the Ion Torrent next-generation sequencing workflow by integrating automated template preparation and chip loading in a single instrument for maximum efficiency and throughput. Simply select the run parameters using the intuitive touch screen interface, load the library samples, reagents, and consumables, and go. 

See how the Ion Chef System helps simplify your sequencing workflow. View now

AIBio Tech® increases workflow efficiency with the Ion Chef System. Watch the #LabChat video and learn how

Ion Chef System workflow

1 Preparation


Ion Chef

  • Load reagents & consumables
  • Load library samples
  • Set run parameters
2 Automated template preparation and chip loading
Ion Chef System

  • Library amplification
  • ISP recovery & enrichment
  • Chip loading

3 Sequencing


Ion PGM, Ion Proton

Run sequence on either:

  • Ion PGM System or
  • Ion Proton System

The Ion Chef System simplifies the next-generation sequencing workflow by integrating automated template preparation and chip loading in a single instrument for maximum efficiency and throughput. Simply select the run parameters using the intuitive touch screen interface, load the library samples, reagents, and consumables, and go.

Compatible with both the Ion PGM and Ion Proton systems, the Ion Chef System supports all Ion chip types, providing flexibility for any project type and size, including use of Ion AmpliSeq libraries for targeted DNA or RNA sequencing.

The Ion Chef™ System supports:

Instrument

Ion Chip

Read Length

Key Research Applications

Ion PGM System

Ion 314™ Chip

Up to 400 bp

Targeted DNA sequencing, targeted RNA sequencing, small RNA sequencing, de novo microbial sequencing, bacterial typing, viral typing, metagenomics, sequencing by genotyping

Ion 316™ Chip

Ion 318™ Chip

Ion Proton System

Ion PI™ Chip

Up to 200 bp

Exome sequencing, transcriptome sequencing, copy number analysis