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Trust next-generation sequencing sample prep solutions from Invitrogen® and Ambion® brand best-in-class reagents for your Roche 454  sequencing experiments.

Step-by-Step Guide to Reagents for the Roche 454® Instrument

DNA


PureLink™ Genomic DNA Mini Kit
Recommended for next generation sequencing applications requiring extraction and purification of genomic DNA samples.  Yields high-quality DNA in a single column.
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RNA


PureLink™ RNA Mini Kit
Recommended RNA purification kit for next generation sequencing applications.  Achieve high quality RNA from a wide arrange of cell and tissue types.
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DNA


SequalPrep™ System
Robust target enrichment by long PCR (5-20kb) for enhanced sequencing of targeted regions, followed by one-step amplicon purification and input equalization in a convenient plate for accurate target representation.
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Cot-1DNA® Blocking Reagent
Increase hybridization sensitivity on array-based exon capture platforms by blocking repetitive sequences.
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RNA


RiboMinus™ RNA-Seq Kits
Enrichment of the true whole transcriptome through selective depletion of 99.9% of ribosomal RNA.
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Epigenome


Magnify™ Chromatin Immunoprecipitation System
Faster and more reproducible solution for Chromatin Immunoprecipitation (ChIP) with the ability to start with lower cell numbers to preserve precious samples.
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MethylMiner™ Methylated DNA Conversion Kit
Enrichment & differential fractionation of dsDNA by CpG methylation density.  Increased sensitivity over antibody methods.
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Cells-to-CpG™ Bisulfite Conversion Kit
Fast & easy conversion of unmethylated cytosines into uracils while methylated cytosines remain unchanged, starting directly from cells, tissue, blood, FFPE, or purified DNA
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DNA Libraries


SOLiD® Fragment Library Barcoding Kits
Unique barcode sequences for SOLiD® fragment libraries to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.
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Ambion® ERCC Spike-In Mixes
Measure sensitivity and quantitate differential gene expression between RNA experiments, including those run on an Next Generation Sequencing instrument.
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