Whole transcriptome analysis is of growing importance in understanding how altered expression of genetic variants contributes to complex diseases such as cancer, diabetes, and heart disease. Analysis of genome-wide differential RNA expression provides researchers with greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression.  With a dynamic range to detect subtle changes in expression level in a hypothesis neutral environment, next generation sequencing enables the understanding of biological response to stimuli or environmental changes.

Step-by-Step Guide to Whole Transcriptome Analysis Sample Prep Products

All Next Generation Sequencing Platforms

PureLink™ RNA Mini Kit
Recommended RNA purification kit for next generation sequencing applications.  Achieve high quality RNA from a wide arrange of cell and tissue types
Learn more

All Next Generation Sequencing Platforms

RiboMinus™ RNA-Seq Kits
Enrichment of the true whole transcriptome through selective depletion of 99.9% of ribosomal RNA.
Learn more

SOLiD® Applications

SOLiD® Total RNA-Seq Kit
Fully optimized, validated kit to construct strand-specific libraries for small RNA and whole transcriptome interrogation on SOLiD® System.
Learn more

Illumina® Applications

Ambion® RNA-Seq Library Construction Kit compatible with the Illumina® GAII platform
Fully optimized, validated kit to construct stran-specific libraries for small RNA and whole transcriptome interrogation on the Illumina® GAII Platform.
Learn more

SOLiD® Applications

SOLiD® Library TaqMan® Quantitiation Kit
Enables measurement of amplifiable template in a SOLiD® library for optimal input in emulsion PCR (ePCR).
Learn more

All Next Generation Sequencing Platforms

Ambion® ERCC Spike-In Mixes
Measure sensitivity and quantitate differential gene expression between RNA experiments, including those run on an Next Generation Sequencing instrument.
Learn more

SOLiD® Applications

SOLiD® RNA Barcoding Kits
Unique barcode sequences for small RNA and whole transcriptome libraries generated with SOLiD® Total RNA-Seq Kit or SOLiD® SAGE™ libraries  to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.
Learn more

Connect with Sequencing Groups

Next Generation Sequencing

  • Sign up to receive updates on Next Generation Sequencing
 

Contact Us

Contact your local sales representative

Education

View our videos & Webinars