Whole transcriptome analysis is of growing importance in understanding how altered expression of genetic variants contributes to complex diseases such as cancer, diabetes, and heart disease. Analysis of genome-wide differential RNA expression provides researchers with greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression.  With a dynamic range to detect subtle changes in expression level in a hypothesis neutral environment, next generation sequencing enables the understanding of biological response to stimuli or environmental changes.

Step-by-Step Guide to Whole Transcriptome Analysis Sample Prep Products

All Next Generation Sequencing Platforms

PureLink™ RNA Mini Kit
Recommended RNA purification kit for next generation sequencing applications.  Achieve high quality RNA from a wide arrange of cell and tissue types
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Ordering Information

Catalog # Name Size List Price (USD) Qty
12280050 PureLink® Viral RNA/DNA Mini Kit 50 preps 221.00
12280096A PureLink® Pro 96 Viral RNA/DNA Purification Kit 4 plates 1,156.00

All Next Generation Sequencing Platforms

RiboMinus™ RNA-Seq Kits
Enrichment of the true whole transcriptome through selective depletion of 99.9% of ribosomal RNA.
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Ordering Information

Catalog # Name Size List Price (USD) Qty
A1083708 RiboMinus™ Eukaryote Kit for RNA-Seq 8 preps 554.00
A1083808 RiboMinus™ Plant Kit for RNA-Seq 8 preps 530.00

SOLiD® Applications

SOLiD® Total RNA-Seq Kit
Fully optimized, validated kit to construct strand-specific libraries for small RNA and whole transcriptome interrogation on SOLiD® System.
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Illumina® Applications

Ambion® RNA-Seq Library Construction Kit compatible with the Illumina® GAII platform
Fully optimized, validated kit to construct stran-specific libraries for small RNA and whole transcriptome interrogation on the Illumina® GAII Platform.
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Ordering Information

Catalog # Name Size List Price (USD) Qty
4445374 SOLiD™ Total RNA-Seq Kit 12 preps 1,566.00

SOLiD® Applications

SOLiD® Library TaqMan® Quantitiation Kit
Enables measurement of amplifiable template in a SOLiD® library for optimal input in emulsion PCR (ePCR).
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All Next Generation Sequencing Platforms

Ambion® ERCC Spike-In Mixes
Measure sensitivity and quantitate differential gene expression between RNA experiments, including those run on an Next Generation Sequencing instrument.
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Ordering Information

Catalog # Name Size List Price (USD) Qty
4449639 SOLiD™ Library TaqMan® Quantitation Kit 250 reactions 1,086.00
A12126 SOLiD™ Library qPCR Standard 10 µL 307.00
A12127 SOLiD™ Library TaqMan® qPCR Module 250 reactions 810.00
4456739 ERCC ExFold RNA Spike-In Mixes 1 kit 2,120.00

SOLiD® Applications

SOLiD® RNA Barcoding Kits
Unique barcode sequences for small RNA and whole transcriptome libraries generated with SOLiD® Total RNA-Seq Kit or SOLiD® SAGE™ libraries  to sequence multiple samples on one slide.  Increase throughput and efficiency while cutting cost.
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