• Defined formulation containing human-derived recombinant proteins
  • Supports feeder-free expansion of ESCs and iPSCs in a serum-free or xeno-free culture condition using KnockOut™ SR (KSR) and KnockOut™ SR Xenofree (KSR XF)
  • Supports complete xeno-free workflow from derivation through differentiation and cryopreservation of ESCs and iPSCs
  • Retains pluripotent gene expression and differentiation capability in both serum-free and xeno-free media
  • Manufactured under GMPs

Figure 1. Morphology of iPSCs.

(A) iPSCs were grown in KSR complete medium with KnockOut™ SR Growth Factor Cocktail on Geltrex™-coated dishes; cells exhibited morphology similar to hESCs, characterized by large nuclei and scant cytoplasm. (B) iPSCs were grown in KSR XF complete medium with KnockOut™ SR Growth Factor Cocktail on Geltrex™-coated dishes; cells exhibited morphology similar to hESCs, characterized by large nuclei and scant cytoplasm.



Figure 2. Pluripotent marker expression of iPSCs.
Immunostaining of iPSCs  for the expression of pluripotent marker OCT4 cultured on KSR medium with KnockOut™ SR GF Cocktail. Green color indicates Oct4 expression;  blue color represents DAPI nuclear staining of the same field.



Figure 3. Morphology of hESCs in a feeder-free culture.
BG01V cells were cultured under feeder-free conditions, using KnockOut™ SR XenoFree directly with KnockOut™ SR GF Cocktail on CELLstart™ Matrix in Knockout™ DMEM. At passage 15, BG01V cells were cryopreserved in the same medium and placed in liquid nitrogen storage. Photos show attachment and growth of BG01V when cryopreserved cells were recovered in feeder-free KnockOut™ SR XenoFree Medium, from day 1 (A) through day 3 (B) post thaw.

Components required for Feeder


Free expansion of ESCs and iPSCs in serum-free and xeno-free culture system

Serum-free and Feeder-free expansion of hESCs and hiPSCs Xenofree and Feeder-free expansion of hESCs and hiPSCs
KnockOut SR GF CocktailKnockOut SR GF Cocktail
KnockOut Serum ReplacementKnockOut SR XenoFree
DMEM/F-12KnockOut DMEM
GeltrexCELLstart
bFGFbFGF
2-mercaptoethanol2-mercaptoethanol
DPBSDPBS