Induced Pluripotent Stem Cells (iPSCs)
The discovery in 2006 that human and mouse fibroblasts could be reprogrammed to generate induced pluripotent stem cells (iPSCs) with qualities remarkably similar to embryonic stem cells has created a valuable new source of pluripotent cells for drug discovery, cell therapy, and basic research.
Invitrogen™ products have been an integral part of iPSC research from the initial discovery of iPSCs to current breakthroughs. Our broad technology platform provides optimized tools for each step of the iPSC workflow: from reprogramming somatic cells to iPSC expansion, validation, and differentiation.
Step-by-Step Guide to iPSC Research Products
Reprogramming of somatic cells for Induced Pluripotent Stem cell (iPS cell) generation can be accomplished by a number of technologies.
Life Technologies provides traditional and non-integrating tools for iPSC generation from a range of somatic cell types.
Cell Identity Confirmation
Induced pluripotent stem cell (iPSC) research requires careful attention to culture conditions. Gibco® media and reagents have been at the forefront of pluripotent stem cell research for years.. From KnockOut™ Serum Replacement to Essential 8™ Medium and cell therapy research media, optimized passaging reagents and substrates, Gibco® products deliver culture with confidence.
Engineer your induced pluripotent stem cells to analyze gene and protein expression and study differentiation and disease pathways. Choose from lipid and electroporation based transfection product and a range of cloning technologies including Gateway® and Topo®.
Life Technologies offers a number of high-quality growth factors and cytokines for targeted differentiation of Induced Pluripotent Stem cells (iPS cells). These proteins are extensively tested to ensure high biological activity, high purity, freeze-thaw stability, and structural homogeneity.
In addition, our large portfolio of cell culture media and reagents, along with our custom media services, allow optimal growth, expansion, and storage of differentiated cells.
Validating iPS cells to ensure they are pluripotent or have differentiated into the desired cell lineage is a critical step in your research.
Invitrogen phosphatase detection kits and cell surface antibodies can assay for pluripotency. Applied Biosystems® TaqMan® Array Stem Cell Pluripotency Cards simultaneously analyze 96 gene expression levels by quantitative PCR and TaqMan® Protein Assays enable relative protein quantification of stem cell protein markers from limited quantities of stem cells.