When used together, Essential 6™ Medium and Essential 8™ Medium streamline the generation, expansion, and differentiation of pluripotent stem cells (PSCs) by offering a feeder-free, xeno-free system for all three steps.

Originally developed and published by Guokai Chen et al.1 in the laboratory of James Thomson, Essential 8™ Medium contains only the essential components needed for stem cell culture*. Essential 6™ Medium uses the same base formulation as Essential 8™ Medium, but without TGFβ or bFGF to allow for reprogramming and differentiation.

  • Want to learn more about adopting Essential 6 and Essential 8 media into your stem cell culture system? Send us an inquiry.

“My experience with the Essential 6™ Medium is very positive, iPS colonies are bigger with more defined colony borders comparing to our standard induction“ Timo Otonkoski, Biomedicum Stem Cell Center (BSCC), Finland

Reprogramming

Sku Name Size Price Qty
A1516401 Essential 6™ Medium 500 mL USD 160.00
A1378001 CytoTune®-iPS Sendai Reprogramming Kit 1 pack USD 1,998.00
A14703 Episomal iPSC Reprogramming Vectors 1 tube USD 768.00
A14700 Vitronectin (VTN-N) Recombinant Human Protein, Truncated 1 mL USD 49.00
A1413301 Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix 1 mL USD 44.50
PHG0264 FGF-Basic (AA 1-155) Recombinant Human Protein 10 µg USD 92.50

Expansion of PSCs

Sku Name Size Price Qty
A1517001 Essential 8™ Medium 500 mL USD 195.98
A14700 Vitronectin (VTN-N) Recombinant Human Protein, Truncated 1 mL USD 49.00
A1413301 Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix 1 mL USD 44.50

EB Formation/Differentiation

Sku Name Size Price Qty
A1516401 Essential 6™ Medium 500 mL USD 160.00
A1413301 Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix 1 mL USD 44.50

Want to learn more about using Essential 6™ Medium and Essential 8™ Medium in your stem cell research, send us an inquiry.

Essential 8™ Medium and Essential 6™ Medium are commercialized in partnership with Cellular Dynamics International.

 

Citation:

1Chen G, Gulbranson DR, Hou Z, et al. (2011) Chemically defined conditions for human iPSC derivation and culture. Nat Methods Apr 10.

* Supports growth for >50 passages without any signs of karyotypic abnormalities

For research use only. Not for use in diagnostic procedures.