When used together, Essential 6™ Medium and Essential 8™ Medium streamline the generation, expansion, and differentiation of pluripotent stem cells (PSCs) by offering a feeder-free, xeno-free system for all three steps.

Originally developed and published by Guokai Chen et al.1 in the laboratory of James Thomson, Essential 8™ Medium contains only the essential components needed for stem cell culture*. Essential 6™ Medium uses the same base formulation as Essential 8™ Medium, but without TGFβ or bFGF to allow for reprogramming and differentiation.

  • Want to learn more about adopting Essential 6 and Essential 8 media into your stem cell culture system? Send us an inquiry.

“My experience with the Essential 6™ Medium is very positive, iPS colonies are bigger with more defined colony borders comparing to our standard induction“ Timo Otonkoski, Biomedicum Stem Cell Center (BSCC), Finland

Reprogramming

Expansion of PSCs

EB Formation/Differentiation

Want to learn more about using Essential 6™ Medium and Essential 8™ Medium in your stem cell research, send us an inquiry.

Essential 8™ Medium and Essential 6™ Medium are commercialized in partnership with Cellular Dynamics International.

 

Citation:

1Chen G, Gulbranson DR, Hou Z, et al. (2011) Chemically defined conditions for human iPSC derivation and culture. Nat Methods Apr 10.

* Supports growth for >50 passages without any signs of karyotypic abnormalities

For research use only. Not for use in diagnostic procedures.