The Essential 8™ Media system and accompanying reagents provide the most defined and consistent culture conditions for pluripotent stem cells. Each of the recommended reagents across the following workflow is defined, without animal components, allowing for consistent results every time.

Most Defined and Consistent PSC System


essential 6

CytoTune®-iPS 2.0  Sendai Reprogramming Kit contains a polycistronic vector which offers an increased reprogramming efficiency up to 1.2%

essential 8

Essential 8™ Medium contains only the 8 necessary components needed for stem cell culture enabling more consistent culture

psc

PSC Cryopreservation Kit contains a xeno-free, ready-to-use cryopreservation medium and a chemically defined recovery supplement, RevitaCell™ Supplement, that consistently recovers 50% more viable cells post-thaw

essential 6

Essential 6™ Medium does not contain bFGF which is used to prevent differentiation and maintain pluripotency

NEW Application: Single cell passaging using Essential 8™ Medium

Essential 8™ Medium has been optimized for single cell passaging PSCs in combination with RevitaCell™ Supplement. Together, this system minimizes the impact of stress from single cell passaging – enabling maximum cell viability and providing more efficient recovery of cells while retaining normal morphology, pluripotency, and karyotype.

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RevitaCell™ Supplement shows utility in single cell passaging using TrypLE™ Select Enzyme. iPSCs generated using episomal vectors were passaged using TrypLE™ Select and seeded at a density of 25,000 viable cells/cm2 onto culture plates coated with truncated and recombinant human Vitronectin in Essential 8™ Medium with 1X RevitaCell™ Supplement. Following 24 hour recovery, PSCs were fed with Essential 8™ medium alone for the remainder of the culture.

Normal karyotypes are retained following single cell passaging using Essential 8™ Medium + RevitaCell™ Supplement at each passage for 30 passages after thawing. iPSCs generated using episomal vectors and H9 ESCs were passaged using TrypLE™ Select Enzyme and cryopreserved using PSC Cryomedium. After thawing, cells were recovered using Essential 8™ Medium with 1X RevitaCell™ Supplement for 18 to 24 hours, followed by feeding with Essential 8™ Medium alone until cells reached ~80% confluency. Cells were passaged using TrypLE™ Select Enzyme for a total of 30 passages and recovered post-split using Essential 8™ Medium with 1X RevitaCell™ Supplement for 18 to 24 hours, followed by feeding with Essential 8™ Medium alone for the remainder of their time in culture. Karyotypes were assessed at the end of the 30th passage.

For research use only. Not for use in diagnostic procedures.