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We provide extracellular matrices (ECMs) that are designed to minimize adaptation time, maximize cell performance, and help meet regulatory requirements.

Whether you are culturing your cells on feeder layers or require feeder-free xeno-free conditions for more clinical applications, Gibco® products provide you with all the choices to culture your pluripotent stem cells (PSC) in the right environment.

Figure 1 (left): H9 cells grown on Geltrex® hESC-qualified Ready-To-Use Matrix and Essential™ 8 Medium, stained for Tra-1-81 expression.

Which extracellular matrix is right for you?

  Most commonly used basement membrane matrix for ESC and iPSC cultures Simplified solution for the most commonly used basement membrane Optimized for use with Essential 8™ Medium Optimal for clinical research applications that require human origin materials only

Product name
Recommended applications Feeder and feeder-free culture Feeder and feeder-free cultureFeeder-free and xeno-free culture Feeder-free and xeno-free culture
Recommended medium pairing StemPro® hESC SFM or Essential 8™ Medium StemPro® hESC SFM or Essential 8™ MediumEssential 8™ Medium KnockOut™ SR XenoFree CTS™-supplemented medium
Source originCultured mouse EHS tumor cells;
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Cultured mouse EHS tumor cells;
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Human (recombinant);
defined
Blend of human native proteins;
defined
Storage temperatureFrozen (-20 to -80° C)Refrigerate (2-8° C)Frozen (-20 to -80° C) Refrigerate (2-8°C)
Reagent preparation timeThaw on ice, overnight in refrigerator, dilute 1:100Ready to useThaw at room temperature, dilute 1:100Ready to dilute 1:50
Unit size1 mL or 5 mL50 mL1 mL2 mL
Number of plates coated/unit16x6 well plate from 1 mL vial diluted 1:1008x6 well plates16x6 well plates from 1 mL vial diluted 1:10022x6 well plates from 1 mL vial diluted 1:100
Price per unit $44.29-$201.00$60.00$49.25$236.00
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Attachment Factor (0.1% Gelatin, Cat. No. S006100) may be used for feeder-layer attachment.