4 Media Options, 20+ Protocols, and 50 Years of Innovation

Pluripotent stem cell (PSC) research requires careful attention to culture conditions. To find the right solution for the right environment, tap into peer-reviewed publications, protocols, instructional videos and stem cell experts.

From KnockOut™ Serum Replacement to Essential 8™ Medium and cell therapy research media, Gibco® products deliver culture with confidence.

Request Information

Which Pluripotent stem cell culture medium is right for you?

The standard for serum-free, feeder-based culture of ESCs and iPSCs from multiple species Feeder-free culture with flexibility to optimize bFGF concentration and maintain differentiation capacity of PSCs Optimal feeder-free culture of all human ESC and iPSC lines with minimal lot-to-lot variability Fully documented xeno-free PSC culture reducing the burden in qualifying reagents, facilitating transition, from the bench to the clinic
Order now Order now Order now Order now
Product name KnockOut™ Serum Replacement StemPro® hESC SFM Essential 8™ Medium KnockOut™ CTS™ XenoFree ESC/iPSC Kit
Recommended applications Feeder culture; reprogramming on feeders; differentiation of PSCs Feeder-free culture Feeder-free, xeno-free culture; feeder-free reprogramming/td> Feeder-based and feeder-free xeno-free culture for cell therapy research
Recommended PSC species Multiple species Medium Human Human
Lot-to-lot variability Medium Medium Low Medium
Cell morphology Compact colonies with smooth edges. High nuclear to cytoplasmic ratio Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges Compact colonies with smooth edges. High nuclear to cytoplasmic ratio Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges
Components/unit 1 x 100mL or 500mL 1 x 10ml StemPro® Supplement
1 x 500ml DMEM/F12 with GlutaMax™
1 x 40ml 25% BSA
1 x 10ml Essential 8™ Supplement
1 x 500ml DMEM/F12 with GlutaMax™
1 x 100ml KnockOut™ SR XenoFree CTS™
1 x 500ml KnockOut™ DMEM CTS™
1 x 5ml KnockOut™ GF Cocktail CTS™
Additional components required for complete medium D-MEM/F-12 (1X) with GlutaMAX™-I , NEAA, bFGF, 2-mercaptoethanol. For cost-effective option, choose the KnockOut™ ESC/iPSC Media bFGF and 2-mercaptoethanol No additional components required bFGF CTS™ and 2-mercaptoethanol
Price/500 ml of complete medium $$ (individually)
$ (with kit)
$$ $ $$$$
Stability of complete medium Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks
Specifically designed for cell therapy research
Available Resources
Protocols for culturing PSCs, publications, hands-on training, product finder

Protocols for culturing PSCs

Protocols for culturing PSCs, FAQs, hands-on training

Protocols for culturing PSCs, FAQs, product documentation
Price/unit $ $$$ $$ $$$$