4 Media Options, 20+ Protocols, and 50 Years of Innovation

Pluripotent stem cell (PSC) research requires careful attention to culture conditions. To find the right solution for the right environment, tap into peer-reviewed publications, protocols, instructional videos and stem cell experts.

From KnockOut™ Serum Replacement to Essential 8™ Medium and cell therapy research media, Gibco® products deliver culture with confidence.

Which Pluripotent stem cell culture medium is right for you?

  The standard for serum-free, feeder-based culture of ESCs and iPSCs from multiple species Feeder-free culture with flexibility to optimize bFGF concentration and maintain differentiation capacity of PSCs Optimal feeder-free culture of all human ESC and iPSC lines with minimal lot-to-lot variability Fully documented xeno-free PSC culture reducing the burden in qualifying reagents, facilitating transition, from the bench to the clinic

Product name
Recommended applicationsFeeder culture; reprogramming on feeders; differentiation of PSCsFeeder-free cultureFeeder-free, xeno-free culture; feeder-free reprogrammingFeeder-based and feeder-free xeno-free culture for cell therapy research
Recommended PSC speciesMultiple speciesHumanHumanHuman
Lot-to-lot
variability
MediumMediumLowMedium
Cell morphologyCompact colonies with smooth edges. High nuclear to cytoplasmic ratio Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges Compact colonies with smooth edges. High nuclear to cytoplasmic ratio Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges
Components/unit1 x 100mL or

500mL
1 x 10ml StemPro® Supplement

1 x 500ml DMEM/F12 with GlutaMax™

1 x 40ml 25% BSA
1 x 10ml Essential 8™ Supplement

1 x 500ml DMEM/F12 with GlutaMax™
1 x 100ml KnockOut™ SR XenoFree CTS™

1 x 500ml KnockOut™ DMEM CTS™

1 x 5ml KnockOut™ GF Cocktail CTS™
Additional components required for complete medium D-MEM/F-12 (1X) with GlutaMAX™-I , NEAA, bFGF, 2-mercaptoethanol.
For cost-effective option, choose the KnockOut™ ESC/iPSC Media
bFGF and 2-mercaptoethanol No additional components requiredbFGF CTS™ and 2-mercaptoethanol
Price/500 ml of complete medium $$ (individually)
$ (with kit)
$$ $ $$$$
Stability of complete medium Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks Stable at 4°C for 2 weeks
Specifically designed for cell therapy research yes
Available Resources yes yes yes yes
Protocols for culturing PSCs, publications, hands-on training, product finder
yes
Protocols for culturing PSCs
yes yes yes
Protocols for culturing PSCs, FAQs,
hands-on training
yes yes yes
Protocols for culturing PSCs, FAQs, product documentation
Price/unit $60.00-$283.00$257.00$196.00$391.00-$2,045.00
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