Pluripotent Stem Cell passaging or dissociating can be accomplished by a number of different methods depending on your culture medium and application.

Whether you prefer to passage your cells enzymatically or manually, Gibco® media provides you with choices for PSC subculture.

Which passaging method is right for you?

  Recommended for clump passaging in feeder and feeder-free cultures Standard reagent for clump passaging in feeder and feeder-free cultures Quick and gentle dissociation, recommended for single cell passaging Recommended for clump passaging with feeder-free Essential 8™ Medium Optimal for clinical research applications that require human origin materials only Quick and easy manual passaging tool for feeder and feeder-free cultures

Product name
Recommended applications Passaging in clumpsPassaging in clumpsDissociation to single cellsPassaging in clumpsPassaging in clumps or single cell passagingMechanical passaging in clumps
Source originBacillus polymyxaClostridium histolyticumMarineChemical animal-origin freeRecombinant enzyme animal-origin freeNot applicable
FormLyophilized powder Lyophilized powder Liquid, ready to use Liquid, dilute 1,000x Liquid, ready to use Individually wrapped
Storage Temperature2-8°C2-8°C 2-8°CRoom temperature Room temperature Room temperature
Shelf life of liquid format 2 weeks2 weeks2 years2 years2 yearsNot applicable
Inactivation method Inhibition by dilution (no inhibitors needed)Inhibition by dilution (no inhibitors needed)Inhibition by dilution (no inhibitors needed)Inhibition by dilution (no inhibitors needed)Inhibition by dilution (no inhibitors needed)Not applicable
Unit size 5 g 1 g 100 mL 4 x 100 mL 100 mL Box of 10 units
Optimized concentration for use 2mg/mL 1mg/mL for feeder-dependent,
10mg/mL for feeder-free
1X Solution 0.5 mM 1X Solution Not applicable
Number of plates passaged/unit 1,250 x 60mm plates 500 x 60mm plates at 1mg/mL,
50 x 60mm plates at 10mg/mL
50 x 60mm plates 50,000 x 60mm plates 50 x 60mm plates 10 plates
Unit price $526.00 $212.00 $36.94 $62.00 $35.87 $193.00
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