Validating cell line quality is critical to stem cell research. Therefore, we offer you maximum flexibility in antibody and dye choices to enable you to specifically detect your pluripotent stem cells (PSCs) and their derivatives.

Superior imaging for PSCs in one box

Everything you need for superior image-based analysis of hPSCs and a variety of lineages is now available in one box. Introducing Molecular Probes® Immunocytochemistry and live imaging kits for stem cells. Kits offer a combination of antibodies, stains, buffers, and/or media to create beautiful, high-quality images of stem cells.

Figure 1. Analysis of OCT4 and SSEA4 expression in iPSCs using the PSC 4-Marker ICC Kit. Induced pluripotent stem cells (iPSCs) derived from CD34+ cord blood (Gibco® Human Episomal iPSC Line, Cat. No. A18945) were grown under feeder-free conditions using Essential 8™ Medium (Cat. No. A1517001) in wells coated with vitronectin (Cat. No. A14700). The cells were stained for pluripotency markers using the PSC 4-Marker ICC Kit (Cat. No. A24881).



 

Figure 2. Surface marker analysis of feeder-dependent PSC cultures using dye-conjugated antibodies for TRA-1-60  (red) as a positive marker and CD44 (green) as a negative marker for pluripotency. 

Figure 3. iPSCs were differentiated for 14 days using the PSC Cardiomyocyte Differentiation Kit (A25042SA). The cells were stained using the Human Cardiomyocyte Immunocytochemistry Kit (A25973) for the following markers: NKX2.5 (red) for early cardiac mesoderm and TNNT2/cTNT (green) for cardiomyocytes along with DAPI nuclear DNA counterstaining (blue).

Stand-alone Pluripotent Stem Cell Antibodies

Target Antigen Reactivity Host Clonality Validation Cat. No.
E-Cadherin Human Mouse Monoclonal WB, IHC, IP, IF, FC, ELISA  13-1700
TRA 1-60 Human Mouse Monoclonal WB, IHC, IF, FC, ELISA 41-1000
TRA 1-81 Human Mouse Monoclonal WB, IHC, IF, FC, ELISA 41-1100
SSEA-3/4 Human, Mouse Mouse Monoclonal IHC, IF, FC 41-4000

WB = Western Blot; IHC = Immunohistochemistry; IP = Immunoprecipitation; IF = Immunofluorescence; FC = Flow Cytometry

These primary antibodies can be conjugated directly with Alexa Fluor® dyes or combined with our vast suite of secondary conjugated antibodies. Stained cells can be visualized by the FLoid™ Cell Imaging Station (Figures 1-3).

Figure 1. H9 hESCs were grown on feeders and stained (without fixing) with SSEA-4 primary antibody and an Alexa Fluor® 488–conjugated anti–mouse IgG secondary antibody. The cells were visualized at 100x magnification using a FITC filter.



 

Figure 2. H9 hESCs were grown on feeders and stained (without fixing) with TRA-1-81 primary antibody and an Alexa Fluor® 488–conjugated anti–mouse IgG secondary antibody. The cells were visualized at 50x magnification. Shown is a merged image using phase contrast and a FITC filter.
 

Figure 3. H9 hESCs were grown on feeders, fixed with PFA, stained with TRA-1-60 primary antibody and an Alexa Fluor® 488–conjugated anti–mouse IgG secondary antibody, and counterstained with DAPI nuclear stain. The cells were visualized at 50x magnification. Shown is a merged image using phase contrast, and FITC and Hoescht filters.

For Research Use Only. Not for use in diagnostic procedures.