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Validation is critical to stem cell research. Therefore, we offer you maximum flexibility in antibody and dye choices to enable you to specifically detect your pluripotent stem cells (PSCs).

Pluripotent stem cells can be identified by the expression of specific cell surface antigens such as the glycolipids SSEA-3 and SSEA-4, and the keratan sulfate antigens TRA-1-60 and TRA-1-81. These antibodies have been tested by our scientists to identify embryonic and induced pluripotent stem cells (Table 1).

Table 1. Pluripotent Stem Cell Antibodies

Target Antigen Reactivity Host Clonality Validation Cat. No.
E-CadherinHumanMouseMonoclonalWB, IHC, IP, IF, FC, ELISA 13-1700
TRA 1-60HumanMouseMonoclonalWB, IHC, IF, FC, ELISA41-1000
TRA 1-81HumanMouseMonoclonalWB, IHC, IF, FC, ELISA41-1100
SSEA-3/4Human, MouseMouseMonoclonalIHC, IF, FC41-4000

WB = Western Blot; IHC = Immunohistochemistry; IP = Immunoprecipitation; IF = Immunofluorescence; FC = Flow Cytometry

These primary antibodies can be conjugated directly with Alexa Fluor® dyes or combined with our vast suite of secondary conjugated antibodies. Stained cells can be visualized by the FLoid™ Cell Imaging Station (Figures 1-3).

Ordering Information

Sku Name Size Price Qty
131700 E-Cadherin Monoclonal Antibody, Mouse (HECD-1) 100 µg USD 380.00
411000 TRA-1-60 Monoclonal Antibody, Mouse (cl.A) 100 µg USD 299.00
411100 TRA-1-81 Monoclonal Antibody, Mouse (clone cl.26) 100 µg USD 299.00
414000 SSEA4 Mouse Monoclonal Antibody (clone MC813-70) 100 µg USD 299.00

Figure 1. H9 hESCs were grown on feeders and stained (without fixing) with SSEA-4 primary antibody and an Alexa Fluor® 488–conjugated anti–mouse IgG secondary antibody. The cells were visualized at 100x magnification using a FITC filter.



 

Figure 2. H9 hESCs were grown on feeders and stained (without fixing) with TRA-1-81 primary antibody and an Alexa Fluor® 488–conjugated anti–mouse IgG secondary antibody. The cells were visualized at 50x magnification. Shown is a merged image using phase contrast and a FITC filter.
 

Figure 3. H9 hESCs were grown on feeders, fixed with PFA, stained with TRA-1-60 primary antibody and an Alexa Fluor® 488–conjugated anti–mouse IgG secondary antibody, and counterstained with DAPI nuclear stain. The cells were visualized at 50x magnification. Shown is a merged image using phase contrast, and FITC and Hoescht filters.

For Research Use Only. Not for use in diagnostic procedures.