Cultured stem cells are vulnerable to contamination by adventitious organisms, such as viruses and bacteria (such as Mycoplasma). Once cell lines are infected, they may undergo spontaneous differentiation or altered function due to adaptation, which may have a profound impact on experimental results. Stem cell laboratories are encouraged to monitor their stem cell cultures. TaqMan® probe-based real-time PCR assays provide a simple and highly sensitive method for detecting specific microorganisms in cell lysates. Compared to traditional methods, such as enzymatic activity or turbidity, TaqMan® Assays offer several advantages, including:

  • Sequence-specific PCR primers and probes ensures greater discrimination in detecting target pathogens in complex biological samples.
  • Simplified workflow with one standard protocol for multiple organisms.
  • Highly sensitive to detect down to 1 colony-forming unit.
  • Rapid turnaround time with results in less than 4 hours.

The AuthentiFiler™ PCR Amplification Kit is an effective tool that uses short tandem repeats (STR) genetic loci to confirm the genetic identity of a human cell.  As demonstrated by Cabera et. al as well as by other researchers in numerous other publications, STR loci can be an effective way to identify cross contamination events involving two or more human cell lines.

Mycoplasma Detection

From the leader in real-time PCR, the Applied Biosystems Myco Scan Kit uses real-time PCR to accurately detect Mycoplasma, the most common pathogen infecting high-value cell lines. Often referred to as the “stealth invader” or “silent killer”, Mycoplasma can destroy valuable cell lines and invalidate research results produced using infected cells. Mycoplasma screening is a critical component of the quality control procedures used to protect high-value cell lines such as stem cell lines.

Life Technologies is the leading provider of MycoSEQ Mycoplasma Detection Kits to pharmaceutical companies using cell lines for antibody production and is designed to meet the recommendations set by the European Pharmacopeia for Mycoplasma detection. Now, Myco Scan is available for researchers that want the same high quality results as MycoSEQ, but with a price tag and configuration tailored to research labs.

  • Confidence—maintain mycoplasma-free cell lines with the sensitivity and specificity offered only by real-time PCR
  • Speed—sample-to-answer in under five hours
  • Coverage—detect more than 90 Mycoplasma species with a single assay

Step-by-Step Guide to Cell Line Contamination

Extract nucleic acid from your cells using the protocol of your choice.

Ordering Information

Catalog # Name Size List Price (USD) Qty
4428176 PrepSEQ® Nucleic Acid Extraction Kit for Food and Environmental Testing 300 preps 1,572.00

Design custom assays for microbial detection or use the MycoSEQ Myco Scan kit for mycoplasma detection.

Applied Biosystems offers more than 1,000,000 TaqMan® Gene Expression Assays, the most comprehensive set of pre-designed Real-Time PCR assays available. All TaqMan Gene Expression Assays have been designed using our validated bioinformatics pipeline, and run with the same PCR protocol, eliminating the need for primer design or PCR optimization.

Look for TaqMan® Gene Expression Assays

Ordering Information

Catalog # Name Size List Price (USD) Qty
4452222 PrepSEQ® 1-2-3 Nucleic Acid Extraction Kit 100 preps 689.00

Ordering Information

Catalog # Name Size List Price (USD) Qty
4376598 StepOnePlus™ Real-Time PCR System, laptop 1 system Request a Quote  
4376599 StepOnePlus™ Real-Time PCR System with Tower 1 system Request a Quote  
4376600 StepOnePlus™ Real-Time PCR System 1 instrument Request a Quote  
4351104 7500 Real-Time PCR System, laptop 1 system Request a Quote  
4351105 7500 Real-Time PCR System, desktop 1 system Request a Quote  
4359284 Tower Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems 1 kit Request a Quote  
4359286 Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems 1 kit Request a Quote  
4362143 7500 Fast System Upgrade Kit 1 kit Request a Quote  
4377354 7500 System SDS 21 CFR Part 11 Module 1 kit Request a Quote  
4317596 ABI PRISM® 7900HT Sequence Detection System User's Manual 1 manual 187.00
4329001 7900HT Fast Real-Time PCR System with 384-Well Block Module 1 instrument Request a Quote  
4329002 7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory 1 instrument Request a Quote  
4329003 7900HT Fast Real-Time PCR System with Standard 96-Well Block Module 1 instrument Request a Quote  
4329007 7900HT System Automation Accessory Upgrade 1 upgrade 45,430.00
4331405 7900HT System Standard 96-Well Block Upgrade Kit 1 kit Request a Quote  
4331406 7900HT System Standard 384-Well Block Upgrade Kit 1 kit 5,950.00
4351402 7900HT System Fast 96-Well Block Upgrade Kit 1 kit 5,950.00
4351405 7900HT Fast Real-Time PCR System with Fast 96-Well Block Module 1 instrument Request a Quote  
4351412 7900HT Fast Real-Time Service Upgrade 1 upgrade 11,060.00
4362078 7900HT Fast Real-Time Service Upgrade with Computer 1 upgrade 12,560.00

For research use only. Not intended for any animal or human therapeutic or diagnostic use.