Epigenetic factors are known to play a critical role in regulating stem cell differentiation by controlling when and how transcription factor bind to promoters or other regulatory elements. Understanding this regulatory mechanism will provide insight into how cells, containing the same genetic material, exhibit significantly different characteristics.

Genetic modifications include the following:

  • Genomic imprinting
  • X-chromosome inactivation
  • DNA methylation
  • Histone modifications

These modifications possess unique molecular signatures that are characteristic by cell state (self-renewal vs. differentiated) or cell type (embryonic, early progenitors or lineage specific cells).

DNA Methylation Analysis is used to identify patterns of methylation by relying on bisulfite treatment of methylated genomic DNA to convert unmethylated cytosine to uracil. Methylated cystosine residues remain unchanged. Treated or untreated samples can be directly compared to identify methylated sites and unique patterns of methylation within a site.

The Life Technologies methylSEQr™ Bisulfite Conversion Kit Workflow is based on PCR amplification and direct sequencing to offer greater resolution to identify unique molecular signatures. The methylSEQr™ kit protocol consists of the following:

Experimental Workflow: Find the Products You Need for Every Step

Design high quality PCR primers for methylation mapping experiments using Methyl Primer Express® Software v1.0. Simply cut and paste in your region of interest. The tool searches for CpG islands and simulates bisulfite modification of DNA in silico. Life Technologies supplies all the tools you need to complete methylation mapping experiments.

Ordering Information

Sku Name Size Price Qty
4376041 Methyl Primer Express® Software v1.0 1 software Contact Us  

The methylSEQr™ Bisulfite Conversion Kit converts non-methylated cytosines (C) in your DNA sample to uracil (U). This kit is an initial step in a multi-step workflow that enables you to determine which cytosines in your sample are methylated by comparing the sequences of treated vs. non-treated DNA.

Ordering Information

Sku Name Size Price Qty
4445553 Cells-to-CpG™ Bisulfite Conversion & Quantitation Control Kit 1 kit USD 310.00
4445554 Cells-to-CpG™ Bisulfite Conversion Kit 192 reactions USD 441.00
4445555 Cells-to-CpG™ Bisulfite Conversion Kit 50 reactions USD 254.00

Identify Unique Molecular Signatures using a Streamlined workflow based on BigDye® Terminator v1.1/v3.1 Sequencing Chemistry, BigDye® XTerminator™ Purification Kits, Applied Biosystems Thermal Cyclers and Genetic Analyzers.

Ordering Information

Sku Name Size Price Qty
4322682 GeneScan™ 500 LIZ™ dye Size Standard 800 reactions USD 440.00
For research use only. Not intended for any animal or human therapeutic or diagnostic use.