Stem cells exhibit unique molecular profiles that are characteristic of cell state, cell type and disease. Gene expression analysis (the quantitative & qualitative measurement of mRNA) allows researchers to investigate several biological activities:

  • Rapid turnaround time with results in less than 4 hours.
  • Highly sensitive to detect down to 1 colony-forming unit in x ml of cell lysate.
  • Sequence-specific PCR primers and probes ensures greater discrimination in detecting target pathogens in complex biological samples.
  • Simplified workflow with one standard protocol for multiple organisms.

TaqMan® probe-based real-time PCR assays are a rapid, sensitive and quantitative method to measure the up and down regulated genes in as little as 1 colony-forming unit. The TaqMan Gene Expression Assays workflow is fully integrated and comprised of five major steps:

Products for the Gene Expression Profiling Workflow

Use fluorescence activated sorter (FACS) to enrich the cell population.

Products for Step 1: Isolate Cells

View more reagents, kits and bench-top devices on beta.lifetechnologies.com

Dynabeads® SSEA-4
hESC can be differentiated into neural-, mesenchymal- and hematopoetic stem cells. This product is intended for the magnetic depletion of SSEA-4+ undifferentiated ESC from a differentiated cell population to increase purity and enable safer cell therapies. The purified differentiated cells are bead- and antibody free and are suitable for any downstream application.

Dynal® CD34 Progenitor Cell Selection System
Use this kit to isolate a high purity and yield of human CD34+ progenitor stem cells. Positively isolated cells are bead and antibody-free, phenotypically unaltered and suitable for any downstream applications including flow cytometry, functional studies and cell culture to produce dendritic cells (DC’s).

The TaqMan® Gene Expression Cells-to-CT™ Kit is a rapid lysis solution for cultured stem cells (10-100,000 cells/sample) that integrates cell lysis and reverse transcription into a single tube. For limited sample, such as single cell analysis, TaqMan® PreAmp Cells-to-CT™ Kit utilizes an intermediate, multiplex pre-amplification step for up to 100 target genes.

Ordering Information

Sku Name Size Price Qty
4391851C Cells-to-CT™ Bulk Lysis Reagents 2,500 reactions ***
4399002 TaqMan® Gene Expression Cells-to-CT™ Kit 40 reactions USD 516.00
4402960 Cells-to-CT™ Stop Solution 1 mL USD 236.00
AM1728 TaqMan® Gene Expression Cells-to-CT™ Kit 100 reactions USD 1,322.00
AM1729 TaqMan® Gene Expression Cells-to-CT™ Kit 400 reactions USD 4,585.00
4387299 TaqMan® PreAmp Cells-to-CT™ Kit 40 reactions USD 2,125.00

Perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification using the TaqMan® PreAmp Cells-to-CT™ Kit. The protocol includes an intermediate amplification step (pre-amplification) between reverse transcription and real-time PCR in which cDNA is enriched for up to 100 gene targets of the user’s choice without amplification bias.

Ordering Information

Sku Name Size Price Qty
4387299 TaqMan® PreAmp Cells-to-CT™ Kit 40 reactions USD 2,125.00
4391851C Cells-to-CT™ Bulk Lysis Reagents 2,500 reactions ***
4402960 Cells-to-CT™ Stop Solution 1 mL USD 236.00

The human and mouse TaqMan® Stem Cell Pluripotency Arrays are comprised of 90 biologically validated stem cell markers and 6 endogenous controls to characterize undifferentiated stem cells or their derivatives. To characterize embryonic or lineage-specific stem cells or assess gene targets in specific pathways, Applied Biosystems offers TaqMan® Gene Expression Assays for 160 commonly-used stem cell differentiation markers. The TaqMan® Gene Expression Master Mix (included with the TaqMan® Cells-to-CT™ kits) has been validated with the TaqMan Gene Expression Assays to ensure reliable quantitation.

TaqMan® Gene Expression Assays

Applied Biosystems offers more than 1,000,000 TaqMan® Gene Expression Assays, the most comprehensive set of pre-designed Real-Time PCR assays available. All TaqMan Gene Expression Assays have been designed using our validated bioinformatics pipeline, and run with the same PCR protocol, eliminating the need for primer design or PCR optimization.

Search for Assays

Ordering Information

Sku Name Size Price Qty
4385344 TaqMan® Array Human Stem Cell Pluripotency Panel 4 cards USD 1,540.00
4385363 TaqMan® Array Mouse Stem Cell Pluripotency Panel 4 cards USD 1,540.00
4369016 TaqMan® Gene Expression Master Mix 1 x 5 mL USD 375.00
4369510 TaqMan® Gene Expression Master Mix 5 x 5 mL USD 1,696.00
4369514 TaqMan® Gene Expression Master Mix 2 x 5 mL USD 704.00
4369542 TaqMan® Gene Expression Master Mix 10 x 5 mL USD 3,290.00
4370048 TaqMan® Gene Expression Master Mix 1 x 1 mL USD 79.25
4370074 TaqMan® Gene Expression Master Mix 1 x 50 mL USD 3,110.00

The TaqMan® Gene Expression Assays workflow is compatible with Applied Biosystems Real-Time PCR Instruments, including the StepOne™, and StepOnePlus™ ,7500, 7500 Fast, 7900HT Fast Real-Time PCR Systems.

Products for Step 5: Quantify

Ordering Information

Sku Name Size Price Qty
4376598 StepOnePlus™ Real-Time PCR System, laptop 1 system Request a Quote  
4376599 StepOnePlus™ Real-Time PCR System with Tower 1 system Request a Quote  
4376600 StepOnePlus™ Real-Time PCR System 1 instrument Request a Quote  
4351104 7500 Real-Time PCR System, laptop 1 system Request a Quote  
4351105 7500 Real-Time PCR System, desktop 1 system Request a Quote  
4359284 Tower Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems 1 kit Request a Quote  
4359286 Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems 1 kit Request a Quote  
4362143 7500 Fast System Upgrade Kit 1 kit Request a Quote  
4377354 7500 System SDS 21 CFR Part 11 Module 1 kit Request a Quote  
4351106 7500 Fast Real-Time PCR System, laptop 1 system Request a Quote  
4351107 7500 Fast Real-Time PCR System, desktop 1 system Request a Quote  
4377355 7500 Fast System SDS 21 CFR Part 11 Module 1 kit Request a Quote  
4397808 High ReSolution Melt (HRM) Software v2.0 1 software USD 3,405.00
4317596 ABI PRISM® 7900HT Sequence Detection System User's Manual 1 manual USD 187.00
4329001 7900HT Fast Real-Time PCR System with 384-Well Block Module 1 instrument Request a Quote  
4329002 7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory 1 instrument Request a Quote  
4329003 7900HT Fast Real-Time PCR System with Standard 96-Well Block Module 1 instrument Request a Quote  
4329007 7900HT System Automation Accessory Upgrade 1 upgrade USD 45,430.00
4331405 7900HT System Standard 96-Well Block Upgrade Kit 1 kit Request a Quote  
4331406 7900HT System Standard 384-Well Block Upgrade Kit 1 kit USD 5,950.00
4351402 7900HT System Fast 96-Well Block Upgrade Kit 1 kit USD 5,950.00
4351405 7900HT Fast Real-Time PCR System with Fast 96-Well Block Module 1 instrument Request a Quote  
4351412 7900HT Fast Real-Time Service Upgrade 1 upgrade USD 11,060.00
4362078 7900HT Fast Real-Time Service Upgrade with Computer 1 upgrade USD 12,560.00