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Protein biomarkers are routinely used to characterize stem cells by cell state, cell type and function. Methods such as immunostaining, Western Blot analysis and FACS have been used to identify, enrich, localize and quantify singular proteins in pluripotent and differentiated cells. However, future needs in stem cell biology require greater throughput and sensitivity to enable global and target-specific analysis. An example of a new target-specific analysis approach is our new TaqMan® Protein Assays.

Step-by-step Guide to Protein Expression

Identification may be accomplished using the following methods:

  • Target-Specific Protein Assays, specific markers of pluripotency and differentiation may be monitored
  • Flow Cytometry, rare populations of differentiated cells among large population of pluripotent cells may be identified

Prepare cell lysates for protein assay analysis utilizing the Protein Expression Sample Prep Kit containing the reagents required for the preparation of target and control cell lysates for use with TaqMan® Protein Assay experiments.

Characterization may be accomplished using the following methods:

  • Target-Specific Protein Assays, specific markers of pluripotency and differentiation may be monitored
  • Flow Cytometry, rare populations of differentiated cells among large population of pluripotent cells may be identified
  • Western Blot Analysis & Immunostaining, a versatile method to assay for protein markers of pluripotency or differentiation pathways, and could be correlated with gene expression analysis and methylation profiling.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.