Reprogramming somatic cells to induced pluripotent stem cells is a critical and potentially time intensive step in your stem cell research. That’s why Life Technologies offers choices in integration-free reprogramming technologies and services that fit your research and resource needs – you can do it yourself or let us do it for you.

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Now available: Essential 6™ Medium for feeder-free reprogramming

Which reprogramming product or service is right for you?

Episomal iPSC Reprogramming Vectors* Epi5™ Episomal iPSC Reprogramming Kit** CytoTune®-iPS Sendai Reprogramming Kit CytoTune®-iPS 2.0 Sendai Reprogramming Kit
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Description Proven technology for viral-free iPSC generation High efficiency viral-free system requiring no small molecules Efficient generation of integration-free iPSCs from multiple somatic cell types Highest efficiency integration-free reprogramming system
Reprogramming Efficiency 0.002–0.08% 0.04–0.3% 0.01-0.5% 0.02 – 1.2%
Genes Utilized Thomson/Yamanaka factors Yamanaka factors +Lin28 Yamanaka factors Yamanaka factors
Method & Medium for Reprogramming Fibroblasts Electroporation using Neon® in N2/B-27 Medium + small molecules Electroporation using Neon® in N2/B-27 Medium Add CytoTune® to cells in fibroblast medium Add CytoTune® 2.0 to cells in fibroblast medium
iPSC Growth Medium Day 15: Transfer to Essential 8™ Medium Day 15: Transfer to Essential 8™ Medium Day 7: Transfer to Essential 8™ Medium or KnockOut™ Serum Replacement supplemented medium Day 7: Transfer to Essential 8™ Medium or KnockOut™ Serum Replacement supplemented medium
Genomic Integration-free
Virus-free Reprogramming
Blood Cell Reprogramming
Transfection Products Neon® Transfection System or similar device Neon® Transfection System or Lipofectamine® 3000 None None
Available in CellModel™ Services
Viral Clearance N/A N/A Up to 15 passages Up to 5 passages
Reporter Available N/A N/A CytoTune®-EmGFP Sendai Fluorescence Reporter CytoTune®-EmGFP Sendai Fluorescence Reporter
*Commercialized in Partnership with Cellular Dynamics International.
**Designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University