Reprogramming somatic cells to induced pluripotent stem cells is a critical and potentially time intensive step in your stem cell research. That’s why Life Technologies offers choices in integration-free reprogramming technologies and services that fit your research and resource needs – you can do it yourself or let us do it for you.

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Now available: Essential 6™ Medium for feeder-free reprogramming

Which reprogramming product or service is right for you?

  Episomal iPSC
Reprogramming Vectors*
Epi5™ Episomal iPSC Reprogramming Kit** CytoTune®-iPS Sendai
Reprogramming Kit
CytoTune®-iPS 2.0 Sendai Reprogramming Kit


Description
Proven technology for viral-free iPSC generation
High efficiency viral-free system requiring no small molecules
Efficient generation of integration-free iPSCs from multiple somatic cell types
Highest efficiency integration-free reprogramming system
Reprogramming Efficiency 0.002–0.08%0.04–0.3%0.01-0.5%0.02 – 1.2%
Genes UtilizedThomson/Yamanaka factorsYamanaka factors +Lin28Yamanaka factorsYamanaka factors
Method & Medium for Reprogramming FibroblastsElectroporation using Neon® in N2/B-27 Medium + small moleculesElectroporation using Neon® in N2/B-27 MediumAdd CytoTune® to cells in fibroblast mediumAdd CytoTune® 2.0 to cells in fibroblast medium
iPSC Growth MediumDay 15: Transfer to Essential 8™ MediumDay 15: Transfer to Essential 8™ MediumDay 7: Transfer to Essential 8™ Medium or KnockOut™ Serum Replacement supplemented mediumDay 7: Transfer to Essential 8™ Medium or KnockOut™ Serum Replacement supplemented medium
Genomic
Integration-free
yesyesyesyes
Virus-free
Reprogramming
yesyes
Blood Cell Reprogramming yesyesyesyes
Special
Equipment
Required
Neon® Transfection System or similar device Neon® Transfection System or similar deviceNone None
Available in CellModel™ Services yesyesyes
Viral Clearance N/AN/AUp to 15 passagesUp to 5 passages
Reporter AvailableN/AN/ACytoTune®-EmGFP Sendai Fluorescence Reporter CytoTune®-EmGFP Sendai Fluorescence Reporter
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*Commercialized in Partnership with Cellular Dynamics International.
**Designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University