TaqMan® hPSC Scorecard™ Panel instrument requirements
Several Applied Biosystems® real-time PCR platforms are compatible with the TaqMan® hPSC Scorecard™ Panel. If you already have any of these installed you just need to upload the template file (.edt or .sdt), and then begin your experiment:
If you are less familiar with the 7500 Fast Real-Time PCR System and Applied Biosystems® real-time PCR platforms, we are happy to help you choose the best instrument for your laboratory’s needs, locate a nearby instrument which you may be able to use, or we can run the hPSC Scorecard™ assay for you with our CellModel™ characterization services.
Cell Model Services
Access the characterization data that the hPSC Scorecard™ Panel provides without access to the instrument. Our CellModel™ Services offer a convenient alternative to obtain the same data, without the need for an instrument.
- Learn more about CellModel™ Services - Stem Cell Characterization
Read your TaqMan® hPSC Scorecard™ panel in 3 easy steps!
1. Load your template file
Download the corresponding template file below and open it in the instrument software. The template file contains all of the necessary cycling parameters and sample names to save you time and make sure the instrument is set up correctly.
2. Insert your plate and Go!
Insert your TaqMan® hPSC Scorecard™ plate into the instrument and start the run.
3. Save your data and analyze
After the run is complete, just save your data in a desired location, and you are done. All of the data analysis can be performed by the TaqMan® hPSC Scorecard™ Data Analysis software
Compatible instrument set-up and support
Check out the instrument set-up guides for your instrument for detailed instruction (with screenshots) on how to read the TaqMan® hPSC Scorecard™ panel. Save Zip file to computer and unzip.
|Template files (.edt)||Step-by-Step User Guide||Instrument manual|
|QuantStudio™ 12K Flex Real-Time PCR System||Download step-by-step guide||Download manual
|StepOnePlus™ Real-Time PCR System||Download step-by-step guide||Download manual|
|ViiA™ 7 Real-Time PCR System||Download step-by-step guide||Download manual
|QuantStudio™ 6 Flex Real-Time PCR system||Download step-by-step guide||Download manual
|QuantStudio™ 7 Flex Real-Time PCR system||Download step-by-step guide||Download manual
|7500 Fast Real-Time PCR System||Download step-by-step guide||Download manual
|7900HT Fast Real-Time PCR System||Download step-by-step guide||Download manual
Need more information?
Need help choosing a PCR instrument? See detailed information here.
Contact us with questions about setting up your instrument or supporting the hPSC Scorecard™ Panel
Frequently asked questions
The Taqman® hPSC Scorecard™ Panel is compatible with the following qPCR systems:
- QuantStudio™ 12K Flex Real-Time PCR System
- ViiA™ 7 Real-Time PCR System
- StepOnePlus™ Real-Time PCR System
- QuantStudio™ 6 Flex Real-Time PCR system
- QuantStudio™ 7 Flex Real-Time PCR system
- 7500 Fast Real-Time PCR System
- 7900HT Fast Real-Time PCR System
Or if you do not have an instrument, we can run the TaqMan® hPSC Scorecard™ panel for you through our CellModel™ characterization services.
What’s in the template files?
The template file contains all of the necessary instrument parameters to run the TaqMan® hPSC Scorecard™ panel. Using the template file not only saves you time, but you can be confident that your instrument is set-up correctly.
What is the difference between a template file (.edt) vs. an experiment file (.eds)?
The template file (.edt or .sdt) provides the thermal protocol and well labels to the instrument’s data collection software. The experiment file (.eds or .sds) contains all of the data from the qPCR run. Please note that in the case of the 7900HT, results must be exported into a .txt file in order to upload into the hPSC Scorecard™ Analysis Software. The software does not recognize the .sds file format.
How long does a run typically take?
The 96-well Fast cycling block enables data collection in little as 30 mins. The 384-well standard block (containing up to 4 samples) typically takes 90 mins. The exact run times of your instrument may vary.
How do I analyze my data?
After you have your experiment file (.eds), proceed to the TaqMan® hPSC Scorecard™ analysis software for data analysis.
My .sds data file from the 7500 is not compatible with the hPSC Scorecard Analysis Software. What can I do?
Download the 7500 2.x software to any computer (it does not have to be connected to the 7500 instrument itself), install the software, import the .sds file and save as an .eds file. Your data file is now compatible with the hPSC Scorecard Analysis Software.