Several Applied Biosystems® real-time PCR platforms are compatible with the TaqMan® hPSC Scorecard™ Panel. If you already have any of these installed you just need to upload the template file (.edt or .sdt), and then begin your experiment:

  • QuantStudio™ 12K Flex
  • ViiA™ 7
  • StepOnePlus™ Real-Time PCR System
  • QuantStudio™ 6/7 Flex Real-Time PCR system
  • 7500 Fast Real-Time PCR System
  • 7900HT Fast Real-Time PCR System

If you are less familiar with the 7500 Fast Real-Time PCR System and Applied Biosystems® real-time PCR platforms, we are happy to help you choose the best instrument for your laboratory’s needs, locate a nearby instrument which you may be able to use, or we can run the hPSC Scorecard™ assay for you with our CellModel™ characterization services.

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Compatible instruments


   

QuantStudio™ 12K Flex
Real-Time PCR System

Maximum throughput, flexibility and scalability

The QuantStudio™ 12K Flex is fully compatible with both the 96-well and 384-well TaqMan® hPSC Scorecard™ panels, allowing the processing of up to 4 samples in a single run.

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StepOnePlus™
Real-Time PCR System

Easy to use for all levels of experience

The 96-well format is easy to use and ideal for researchers running only a few samples at a time. The 96-well fast cycling block enables data collection in little as 30 mins.

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ViiA™ 7
Real-Time PCR System

High flexibility and intuitive
design

The ViiA™ 7 system is fully compatible with both the 96-well and 384-well TaqMan® hPSC Scorecard™ panels, allowing the processing of up to 4 samples in a single run.

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QuantStudio™ 6 Flex Real-Time PCR system

High flexibility & intuitive design

The QuantStudio™ 6 Flex Real-Time PCR System is the ideal instrument for laboratories with project requirements that go beyond a single application or project and need to accommodate the needs of many on a limited budget

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QuantStudio™ 7 Flex Real-Time PCR system

High versatility & intuitive design

The QuantStudio™ 7 Flex Real-Time PCR System delivers the proven reliability, sensitivity and accuracy of the ViiA™ 7 System in a new industrial design

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7900HT Fast Real-Time PCR System

Robotic capabilities

The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading

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7500 Fast Real-Time PCR System

Trusted and fast performance & dye – versatility

Applied Biosystems® 7500 Real-Time PCR Systems offer you high performance, multicolor real-time PCR wherever your research takes you. Choose from our Fast and standard 7500 systems.

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Cell Model Services

Access the characterization data that the hPSC Scorecard™ Panel provides without access to the instrument.  Our CellModel™ Services offer a convenient alternative to obtain the same data, without the need for an instrument.

Read your TaqMan® hPSC Scorecard™ panel in 3 easy steps!

1. Load your template file

Download the corresponding template file below and open it in the instrument software. The template file contains all of the necessary cycling parameters and sample names to save you time and make sure the instrument is set up correctly.

Figure 1. Example of opening new template file on ViiA™ 7 Real-Time PCR System.  Click on browse from the “select experiment template” area and open template file for TaqMan® hPSC Scorecard™ Panel.


2. Insert your plate and Go!

Insert your TaqMan® hPSC Scorecard™ plate into the instrument and start the run.

Figure 2. Inserting a TaqMan® hPSC Scorecard™ plate into the instrument.


3. Save your data and analyze

After the run is complete, just save your data in a desired location, and you are done. All of the data analysis can be performed by the TaqMan® hPSC Scorecard™ Data Analysis software

Figure 3. Screenshot of TaqMan® hPSC Scorecard™ Data Analysis software.

Compatible instrument set-up and support

Check out the instrument set-up guides for your instrument for detailed instruction (with screenshots) on how to read the TaqMan® hPSC Scorecard™ panel. Save Zip file to computer and unzip.

  Template files (.edt) Step-by-Step User Guide Instrument manual
QuantStudio™ 12K Flex Real-Time PCR System Download step-by-step guide Download manual
StepOnePlus™ Real-Time PCR System Download step-by-step guide Download manual
ViiA™  7 Real-Time PCR System Download step-by-step guide Download manual
QuantStudio™ 6 Flex Real-Time PCR system

Download step-by-step guide Download manual
QuantStudio™ 7 Flex Real-Time PCR system Download step-by-step guide Download manual
7500 Fast Real-Time PCR System Download step-by-step guide Download manual
7900HT Fast Real-Time PCR System Download step-by-step guide Download manual


Need more information?

Need help choosing a PCR instrument?  See detailed information here.

Contact us with questions about setting up your instrument or supporting the hPSC Scorecard™ Panel

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Frequently asked questions

Instrument compatibility

The Taqman® hPSC Scorecard™ Panel is compatible with the following qPCR systems:

  • QuantStudio™ 12K Flex Real-Time PCR System
  • ViiA™ 7 Real-Time PCR System
  • StepOnePlus™ Real-Time PCR System
  • QuantStudio™ 6 Flex Real-Time PCR system
  • QuantStudio™ 7 Flex Real-Time PCR system
  • 7500 Fast Real-Time PCR System
  • 7900HT Fast Real-Time PCR System

Or if you do not have an instrument, we can run the TaqMan® hPSC Scorecard™ panel for you through our CellModel™ characterization services.


What’s in the template files?

The template file contains all of the necessary instrument parameters to run the TaqMan® hPSC Scorecard™ panel. Using the template file not only saves you time, but you can be confident that your instrument is set-up correctly.


What is the difference between a template file (.edt) vs. an experiment file (.eds)?

The template file (.edt or .sdt) provides the thermal protocol and well labels to the instrument’s data collection software. The experiment file (.eds or .sds) contains all of the data from the qPCR run. Please note that in the case of the 7900HT, results must be exported into a .txt file in order to upload into the hPSC Scorecard™ Analysis Software. The software does not recognize the .sds file format.


How long does a run typically take?

The 96-well Fast cycling block enables data collection in little as 30 mins. The 384-well standard block (containing up to 4 samples) typically takes 90 mins. The exact run times of your instrument may vary.


How do I analyze my data?

After you have your experiment file (.eds), proceed to the TaqMan® hPSC Scorecard™ analysis software for data analysis.

 

My .sds data file from the 7500 is not compatible with the hPSC Scorecard Analysis Software. What can I do?

Download the 7500 2.x software to any computer (it does not have to be connected to the 7500 instrument itself), install the software, import the .sds file and save as an .eds file. Your data file is now compatible with the hPSC Scorecard Analysis Software.

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