The hPSC Scorecard™ Analysis software is an easy-to-use data analysis tool that utilizes a proprietary algorithm to rapidly and accurately predict pluripotency and lineage bias based on a panel of 94 genes. The software is free to use. Simply save your qPCR data from your instrument, sign in to the Life Technologies website, and upload your data into the analysis software.

Sign in

Need help? Have a question? Find support resources now >

What does it do?

Access, report, and share data in minimal time

Because the software is web based, you can securely access and retrieve data anywhere internet access is available. Once logged in, you can build projects and reports from one or from many experiments to quickly share your results with others.


Features of the software include:

  

Scorecard
A simple-to-interpret summary of gene expression level data that confirms pluripotency or indicate germ layer bias of your sample
 
Suggested Next Steps
Links to information and protocols that may help you decide what to do after using the TaqMan® hPSC Scorecard™ Panel
 
Expression Pattern Plot
Compare gene expression between samples for each of the marker sets
     
  

Correlation Plot
See how gene expression levels correlate between samples
 
Box Plot
Perform a quick quality control check to make sure your sample amplified as expected
 
Reports
Build customized reports to easily communicate your results with collaborators


Identify clones with trilineage differentiation potential

Include randomly differentiated embryoid body (EB) samples, to quickly determine whether your cell line is biased towards one or more of the three germ layers (endoderm, ectoderm, mesoderm)


Confirm pluripotency with minimal effort

Curious if your cells are pluripotent? The hPSC Scorecard™ analysis software not only analyzes the expression of the pluripotency genes, but confirms that your cells are not expressing any germ layer–specific genes.


Compare results to validated content

The contents of the reference panel are based on published work (Bock et al. Cell 144:439–452 (2011)) and were validated against multiple human ES and iPS lines.

How do I use it?

How do I sign in?

Sign in using your current Life Technologies account information. New users to the site can quickly register by clicking the “Register Now” button on the lower half of the page. Following sign in, you will be instantly redirected to the hPSC Scorecard™ analysis software.


How do I upload data?

Once you have created your project, simply click “Upload Data” to start using the analysis software.


Will I need to update my software?

No software update is ever required. Since the hPSC Scorecard™ Analysis software is cloud based, you will have access to the most up-to-date version of the software every time you log-in.


What sample type should I select?

The software allows you to easily evaluate pluripotency and/or inductive differentiation one germ layer at a time. To make it a little easier, a set of common sample types have been already built into the software to allow you to get to your results faster:

  
  • All Pluripotency: Quickly confirm the pluripotency of undifferentiated samples
  • All Ectoderm / All Endoderm / All Mesoderm: Evaluate the inductive differentiation specified to one or more germ layers
  • All Trilineage: Evaluate all three germ layers simultaneously for EB samples
  • Multiple Sample Types: Allows you the flexibility to mix and match samples types for analysis


Do I need to run a new sample if I want to look at a different sample type?

No. The software analyzes and retains all of the qPCR data from the plate upon import. If you want to look at a different sample type, simply select the desired sample type from the drop down list, and the analysis software will automatically update the scorecard results.

Frequently asked questions

What web browsers are compatible with the analysis software?

The hPSC Scorecard™ analysis software is compatible with the following web browsers:

  • Internet Explorer 9
  • Safari®
  • Google® Chrome™


I can’t sign-in to my Life Technologies account, what should I do?

Retrieve your username or password using the appropriate links on the sign-in page.


I don’t see anything when I log-in to the analysis software (or pieces are missing), what should I do?

Confirm that you are using a web browser that is compatible with the hPSC Scorecard™ Analysis software.


My data isn’t being uploaded, what should I do?

The analysis software validates the data file upon import. If your file is not imported (or you receive an error) confirm that you have the correct Instrument and Block type designated in the project window (click the  button). Also verify that you have used one of the following catalog products: A15870, A15876, A15872, A15871. The analysis software will only accept data obtained using those specific panels.


I would like someone to walk me through using the software

Please contact our specialized technical support team.  Email: stemcell-help@lifetech.com


My results don’t look right, what should I do/who should I call?

Please contact our specialized technical support team.  Email: stemcell-help@lifetech.com