Learning Made Easier with Synthetic Biology Webinars

Inquire, Understand, and Break Through to Discovery

At Life Technologies, we believe synthetic biology will change the way we create energy, produce food, optimize industrial processing, and detect, prevent, and cure diseases—improving the human condition and the world around us. We’re committed to offering unparalleled technology and solutions to the research community. With our platform of synthetic biology products, we intend to understand and answer some of life’s most challenging questions. Through design and engineering, our scientists enable researchers to study, alter, create, and re-create highly complex pathways, DNA sequences, genes, and natural biological systems. In the synthetic biology webinar series from Life Technologies, our scientists cover the different challenges that synthetic biology researchers encounter and address the solutions available to help them achieve their next breakthroughs.

Stay at the forefront of synthetic biology breakthroughs, register for a live webinar, or view our library of past webinars at your leisure. New webinars are added monthly, so subscribe now and be among the first to learn about the latest advances in synthetic biology research.

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Date Abstract  
April 9 & 10, 2014 RNAi technology reveals possible targets for treating Parkinson's disease
Gene silencing through RNA interference (RNAi) has revolutionized our ability to interrogate gene function. Since its discovery in Caenorhabditis elegans, RNAi has been established in many additional organisms, including Drosophila and mammalian cells, and methods that exploit RNAi have become important tools for biologists. Over the past decade, numerous siRNA screens have informed areas of human biology ranging from basic cellular processes to complex disease phenotypes. A recent study by Hasson et al. (Nature, 2013) coupled siRNA screening with cells expressing GFP-parkin to identify genes that affect parkin accumulation on damaged mitochondria (a process with relevance to Parkinson’s disease). The presenters will discuss this study to highlight considerations for running large-scale RNAi screens, ranging from the basic concepts and experimental setup to computational and experimental approaches used to overcome the prevalence of off-target effects. They will also provide guidance on approaches to simplify the workflow from RNAi-based screening to validation.
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On demand
RNA-guided genome editing with engineered Type II CRISPR/Cas9 System
Genome editing technique involves the use of engineered nucleases in conjunction with endogenous repair mechanisms to insert, delete, or replace a specific DNA sequence from a desired location in the genome. Recently, the Type II CRISPR/Cas bacterial adaptive immune system from Streptococcus pyogenes has been engineered and demonstrated to function as an effective gene editingtool in various eukaryotic organisms including mammalian cells. Recent publications have also shown thatcatalytically inactive double-knockout Cas9 tethered to effecter proteins such as activators or repressors can be recruited to a desired location on the genome to modulate transcription. With its simple and cost-effective design, along with its broad host application, CRISPR/Cas9 system provides another set of genome editingtool that provides a great opportunity for multiplex gene editing and modulation. Join this webinar to get an overview on CRISPR/Cas9 mediated genome editing and gain insights into Life Technologies GeneArt® CRISPR Nuclease System.


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Date: November 13, 2013
Directed evolution methods for protein engineering

The production of mutant protein libraries using traditional methods is far from perfect; as a result, the majority of the time and the resources in subsequent rounds of screening can be spent pursuing large numbers of irrelevant variants, with a lack of mutations at critical sites available for testing. In this webinar, Dr. Michael Liss, the manager of GeneArt® Directed Evolution service and an expert in rational protein design, illustrates how GeneArt® Directed Evolution technology enables researchers to evolve proteins rationally, minimizing the amount of effort needed to screen the library and maximizing the number of useful variants obtained. 

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Genome editing of iPSCs from Parkinson’s disease patients using TAL technology: design, detect, and quantitate
Be sure to join us for this live webinar as we examine genetic engineering of iPSCs derived from Parkinson’s disease patient samples, which provide model systems to study disease mechanisms in cell types not previously available. Next-generation tools for stem cell engineering, GeneArt™ Precision TALs offer an extremely efficient method for targeting and regulating expression at defined sequences within the genome, enabling you to more accurately engineer and study gene function to develop better stem cell models.
Our webinar panel will:

  • Review the TAL technology and reported applications in stem cells
  • Cover the use of TAL editing in Parkinson’s disease iPSCs
  • Provide you with screening protocols for detecting and quantitating genomic alterations as small as a single nucleotide

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On demand TAL Effectors Tool Box for Precision Genome Editing
Transcription activator–like (TAL) effectors are proteins expressed by plant bacterial pathogens to evade host defense mechanisms. Due to the unique and predictable code for each DNA base within the amino acid repeat regions, TAL DNA binding domains can be custom tailored to bind any DNA sequence of choice. By tethering these customizable DNA binding domains to effector proteins such as activators, repressors, or nucleases, these proteins can be recruited to a desired location on the genome to either modulate transcription or editing of the encoded message. Therefore, designer TAL effectors are attractive tools for genome and cell engineering applications.   Designer TAL effectors are versatile not only in their function, but have also been shown to work in a variety of host systems.  Join this live, interactive webinar, titled “TAL effectors tool box for precision genome editing,” for an introduction to designer TAL effectors and their applications for targeted gene editing
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On demand Combine expression-optimized genes with an advanced expression system to improve your results
Gene optimization affects multiple levels of gene expression, and results in increased overall expression reliability and protein yields. This complete solution—from gene to protein—offers enhanced protein expression with different options, depending on your level of experience and lab resources. This includes the option to outsource your gene expression, receiving your protein within 30 business days, or adopting the technologies in your own laboratory.
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On demand New Easy Methods for Next Generation Cloning for Every Lab
This webinar showcases a new methodology for cloning genes using GeneArt® Strings™ DNA Fragments along with the trusted assembly techniques of restriction enzyme/ligation and seamless cloning. This new methodology not only helps save time and money but allows scientists to remain focused on results rather than troubleshooting lengthy cloning reactions.
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On demand Introduction to simplified vector design and integrated gene synthesis ordering through new Vector NTI® Express Designer Software
Vector NTI® Express Designer Software leads the way in engineering the next generation of in silico cloning and assembly. The newly developed interface is a virtual project canvas for both simplified drag-and-drop vector construction and simplified submission and ordering of gene synthesis projects.  In this webinar, you will learn step-by-step how this powerful, easy-to-use bioinformatics software can help you do bio-design, faster customer vector construction, sequence optimization, and gene synthesis.
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On demand Synthetic TAL Effectors for Targeted Gene Activation in Plants
The ability to selectively activate or inhibit gene expression has value in basic plant biology and agriculture. Targeted gene activation can be achieved by specifically expressing a gene of interest in a temporally and/or spatially defined manner in transgenic plants, or by directly activating endogenous gene expression in its original genetic context. Join this webinar where Dr. Neal Stewart, professor and director at Tennessee Plant Research Center provides novel insights into the potential applications of synthetic TALEs for targeted gene activation of transgenes in plants.
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Fast, simple alternative to traditional cloning
This webinar illustrates how a significant amount of time could be saved by cutting out the unnecessary steps from traditional cloning and moving into gene synthesis. Gene synthesis has become a cost effective, time- and resource-saving method for obtaining nearly any desired DNA construct with 100% accuracy. It outperforms conventional molecular biology techniques in terms of time and cost, while providing equivalent or better expression performance, and construct stability and quality. Watch this webinar and go beyond traditional synthesis and build your next breakthrough using next-generation cloning.

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On demand Move Beyond Traditional DNA Cloning and Editing
In this webinar, learn how to clone or edit DNA in a single step harnessing the power of homologous recombination. No more restriction digests, end-repair, ligation, subcloning, and enzyme inactivation steps with DNA loss at every stage. Even multisite mutagenesis is made simpler with homologous recombination, making substitutions, deletions, or insertions easier than ever.  Watch this webinar and you’ll cut, edit and create DNA faster than ever.
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On demand Plant genetic engineering
This webinar introduces the newest genome editing technology for plants, GeneArt® Precision TALs. The scientific presentation also covers other cloning and algae engineering technologies, such as Gateway® and GeneArt® gene synthesis, to support your quest to engineer the next generation of crops.
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Plant sequencing
In a paper in the journal Plant Physiology, Dr. Franziska Turck and her team explained how they recently introduced deep candidate resequencing (dCARE) to their Arabidopsis mutant identification pipeline using the Ion PGM™ Sequencer. In this webinar, learn Dr. Turck’s opinion about the application of the isogenic mapping approach for plant gene identification, and how the fast and cost-effective barcoding Ion PGM™ System improves her deep sequencing workflow.

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Genetic analysis solutions for plant science
This webinar discusses scientific examples from key plant biotechnology applications such as transcriptome sequencing, plant genotyping and marker-assisted selection, plant genetic engineering, and GMO testing. The examples are followed by a presentation on how our simple, scalable, and affordable genetic analysis solutions can have the greatest impact for plant researchers.

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Meet the Inventor

Dr. Federico Katzen
GeneArt® DNA Assembly

Dr. Jon Chesnut
Gateway® Technology

Dr. Jon Chesnut
GeneArt® Precison TALs

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