Our Value Oligos are the most economical and fastest way to order oligos from Life Technologies. You can order Value Oligos as 25 nmol or 50 nmol in a tube format with a range of purification options to suit your applications. However, if your research demands a scale larger than 50 nmol, different format, or 5', 3', or internal modifications, then our Standard Oligos have you covered.

Just like our Value Oligos, our standard custom DNA Oligonucleotides are synthesized on a highly automated, computer-controlled system using cyanoethyl phosphoramidite chemistry and subject to rigorous quality control procedures.

Synthesis Scales

Our custom DNA Oligos are available at 25 nmol, 50 nmol, 200 nmol, 1 µmol and 10 µmol scales. Visit our Minimum Yield Guarantee page to see explanations of our minimum yield guarantee and estimated yields.

Plate Formats

In addition to tubes, our custom DNA Oligos are available in 96- or 384-well plate formats, with the same purification, synthesis scale, plate type and volume, shipping medium, and buffer type for the entire order.

Modifications

5', 3', or internal modifications are available. Routine 5' modifications include biotin, phosphate, and Primary Amine®. In addition to modifications with fluorescent dyes, such as fluorescein, HEX, and TET, we offer a wide range of Molecular Probes® dye modifications, such as Alexa Fluor® and BODIPY® dyes. For a full list of available modifications, visit our Modification Options page.

Which Kind of Oligos are Right for Me?

Value Oligos Standard Oligos
  • Price is calculated per oligo
  • Price is calcualted per base
  • Length: 5-40 mers
  • Scale: 25-50 nmol
  • Length: 5-100 mers
  • Scale: from 25 nmol to 10 µmol
  • Not available with modifications
  • 5', internal, and 3' modifications are available
  • Eligible for standard and next-day delivery
  • Standard delivery only

Choosing the Right DNA Oligos for Your Application

Oligonucleotides are the starting point for many of today’s biology research, drug discovery and diagnostics applications. These highly technical applications demand high-quality oligonucleotides for success. Custom DNA oligos at Life Technologies are synthesized on a highly automated, computer-controlled system using standard cyano-ethyl phosphoramidite chemistry. Coupling efficiencies are monitored throughout synthesis of each oligonucleotide by trityl analysis, ensuring the quality of the process, not just the end product. Post-synthesis QC, mass spectrometry for short oligos and capillary electrophoresis (CE) for long oligos, ensure quality.

Choosing the synthesis scale and purification option that is best for your application depends on the nature of your downstream applications. The table below is designed to help you choose the right oligos and purification methods for your application.

In general, Cartridge purification is fast and economical but may compromise purify or yield depending on the oligo sequence (sequences with 5’ G’s can be problematic). HPLC is more time consuming but gives excellent purity up to 55 bases. Above 55 bases, it becomes increasingly difficult to differentiate full-length product from n-1 failure sequences. PAGE gives excellent resolution even with very long oligos but generally sacrifices yield for quality.

 

Application

Desalted

Cartridge

HPLC

PAGE

 

  • 25 nmole – 10 µmole
  • 5 – 100 bp
  • Removes salts but not failure
  • 50 nmole – 1 µmole
  • 7 – 55 bp
  • Removes failure sequences
  • Provides 80% full-length sequence
  • 50 nmole – 10 µmole
  • 10 – 55 bp
  • Removes failure sequences
  • Provides >85% full-length sequence
  • 50 nmole – 10 µmole
  • 7 – 100 bp
  • Removes failure sequences
  • Provides > 90% full-length sequence

Standard PCR

  

 

Specialty PCR

  

Cloning

  

cDNA Library Construction

  

Sequencing

  

 

Fluorescent Sequencing

 

 

 

Next-Generation Sequencing

  

Mutagenesis

  

Antisense

  

 

Gel Shift Assays

 

 

Microarrays

 

 

 

For Research Use Only. Not for use in diagnostic procedures.