The TOPO® Cloning Advantage

Invitrogen's TOPO® cloning technology simplifies and improves PCR cloning by replacing ligase and long ligation times with a cloning vector that has been “activated” with topoisomerase I. This activated vector supports room-temperature ligation in 5 minutes and yields a very high percentage of recombinants. Simply put, no other cloning technology is as fast or as efficient.

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For more information or to order a service, call 800.955.6288 x2, or email custom.services@lifetech.com

How It Works

Practically any vector you choose can be adapted for TOPO® cloning of PCR products at high efficiencies. Our scientists will design the cloning strategy and review it with you, modify your vector by covalently attaching the topoisomerase I enzyme, and prepare the vector ends for either blunt or TA Cloning® vectors. If you choose, we can also prepare your vector for directional cloning of PCR products. Each vector is functionally tested in TOPO® cloning reactions to ensure guaranteed cloning efficiencies.

High-Throughput Compatible
Functionally tested TOPO®-adapted custom vectors are supplied in bulk, for a minimum of 500 reactions, so they are HTP compatible. In addition, you'll receive high-efficiency competent cells in bulk or in a 96-well format for HTP cloning. You won't have to gather additional reagents to complete your cloning projects—so you'll save even more time.

Activate YOUR Vector!

Invitrogen supplies numerous TOPO® vectors for many common cloning needs. We can also TOPO®-adapt any custom vector. If you are identifying, cloning, and expressing hundreds or thousands of PCR-amplified genes in your high-throughput (HTP) experiments, save many hours of time by allowing us to adapt your preferred vector with TOPO® technology. The advantages of our service are:

  • No need to switch protocols and reagents to a standard TOPO® vector—you can continue to use the vector that best suits your cloning needs, yet still enjoy the advantage of 5-minute ligations at room temperature
  • Greatly increased cloning efficiency over non-adapted vectors
  • No need to change any downstream assays or experimental parameters