Eukaryotic protein expression

When a functional target protein is required and other expression systems are not amenable, Invitrogen offers a baculovirus expression option in three proprietary insect cell lines.

Overview

Baculovirus expression systems offer high-level expression, ease of scale-up, and production of recombinant protein with posttranslational modifications. Invitrogen evaluates protein expression in Sf9, Sf21, and High Five™ suspension culture cell lines.
A titerless viral supernatant infection method helps to ensure that the optimal host and expression conditions are identified in real time and reproducible in scale up to 200 L.

Viral amplification
Generation of recombinant bacmid and high-titer viral supernatant

Small-scale expression studies
A viral supernatant dilution scheme of at least three different volumes is added to 50 mL of Sf9, Sf21, and High Five™ insect cell cultures. Standardized cell culture aliquots are removed at 0, 24, 48, and 72 hours and evaluated for cell density, viability, and size over time. The optimal expression conditions are identified via western blot analysis.

Expression scale-up
From 1 L to 200 L

Protein purification services
Affinity, ion exchange, HIC, SEC, and other purification methods employed

FAQs

What advantages does a baculovirus expression system provide?
Baculovirus expression systems can be used when bacterial expression is not feasible within a bacterial expression system, and also when a target protein with posttranslational modifications is needed for functional and structural studies.

Is baculovirus infectious to humans?
No, it is completely safe but still enables protein expression within a eukaryotic system.

How long does a baculovirus expression project usually take?
Most services, starting with the baculovirus expression plasmid, can be completed in 6–7 weeks.

Contact Information

For further information, please contact Invitrogen Custom Services