FreeStyle™ 293 Mammalian Protein Expression

To get the best results in your downstream applications requiring mammalian proteins, you need proteins with post-translational modifications and/or proper folding. The FreeStyle™ 293 Expression Service rapidly provides you with large amounts of functional mammalian recombinant proteins. This service uses the FreeStyle™ Expression System, which is a complete, suspension cell-culture system utilizing specially-formulated, defined serum-free medium. The FreeStyle™ 293 Expression Service offers the following benefits over other expression systems:

Mammalian cell advantages

  • Mammalian post-translational modifications and/or proper folding
  • High levels of expression
  • Rapid transient expression of proteins (3-6 weeks)
  • Defined serum-free medium
Services Offered
Invitrogen’s FreeStyle™ 293 Mammalian Expression Service can be optimized to rapidly produce your mammalian protein. We offer the following services to help you with your mammalian protein expression needs:

  • Clone your gene of interest into a mammalian expression vector
  • Time course experiments to determine the optimal time of expression for your protein of interest
  • Verify expression of the gene of interest by SDS-PAGE or Western Analysis
  • Optimize expression and scale-up
  • Purification
Service Timeline
FreeStyle™ Time Course 3 weeks
FreeStyle™ 293 Expression 1 Liter 3 weeks
FreeStyle™ 293 Expression 5 Liters 3 weeks
FreeStyle™ 293 Expression 25 Liters 3-4 weeks
FreeStyle™ 293 Expression 50 Liters 4-6 weeks
Concentration of 5-20L of FreeStyle™ supernatant medium 1-2 weeks
Concentration of 21-100 L of FreeStyle™ supernatant medium 1-2 weeks
Custom FreeStyle™ Mammalian Expression Services inquire