The SelectScreen® Cell-based GPCR Profiling Service utilizes Invitrogen’s growing portfolio of division arrested or cryo-preserved GeneBLAzer® and Tango™ functionally validated assays for GPCRs.

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It offers a flexible approach to screening:

  • Obtain rapid profiling of your compounds against a panel of GPCRs at a single concentration, or
  • Have more in-depth EC50/IC­50 dose response determinations performed.

You also chose whether you want your compounds profiled in agonist or antagonist mode. All of our functional cell lines are offered in both modes. Counter-screen your “hits” against our parental cell lines when utilizing our GeneBLAzer® platform to evaluate non-specific effects.

Overview of GeneBLAzer® Technology

Overview of Tango™ Technology

SelectScreen® GPCR Profiling Service Panel Selections

This service offers all of the following:

  • High quality data
  • 2-3 week turnaround
  • Competitive pricing
  • Complete confidentiality
  • Open business model – NO minimum commitment

SelectScreen® Cell-based GPCR Profiling Service Overview

Built-in Quality

A stringent validation process ensures the highest quality data possible. Each data point is performed in duplicate. Strict quality control protocols ensure that any assay results not meeting set specifications will be automatically repeated. EC50 or IC50 value determinations for each GPCR are performed with known agonist and/or antagonist (when available) to determine data validity and consistency.

More detail around the protocol, specifications, and controls can be obtained in our customer protocol and assay conditions guide, which is available in the PDF downloads on the right.

All of the functional, GPCR-specific GeneBLAzer® and Tango™ cell lines used in the SelectScreen® Cell-based GPCR Profiling (SSCG) Service provide superior response profiles as a result of optimization of assay conditions and selection of high-responding cell populations. All GeneBLAzer® and Tango™ cell lines in the portfolio were researched and developed by Invitrogen; we ensure the consistency, reliability, and performance of each cell line. In fact, the same cell lines that we use in our service can be purchased by our clients to perform internal follow-up experiments. GPCR-specific GeneBLAzer® and Tango™ cell lines:

  • Are division arrested or cryo-preserved to avoid variability associated with cell division
  • Provide ready-to-screen, ratiometric assays for disease relevant targets
  • Are functionally validated to ensure high-quality results each and every time

The GeneBLAzer® and Tango™ cell lines in the Service are tested and documented to show a high level of performance. These assays meet the following specifications:

  1. Z´-factor of 0.5 or greater for agonist assays and Z´-factor of 0.4 or greater for antagonist assays
  2. Appropriate EC50/IC50 responses to known agonists and antagonists

Listed below are the GPCR targets available for profiling.  Our list of targets represents a cross section of GPCR families, with most of the GPCRs falling into the Class A category of GPCR targets, with a smaller number in Class B. We will continue to expand our panel of GPCR targets, with emphasis on a broad representation of as many families as possible.  Check back often for updates to this list.

If your target of interest is not on the list below, additional catalog GPCR cell lines may be profiled using our Custom Services.   View the entire list of GPCR cell lines available.

If you identify a GPCR target you are interested in screening, please contact us at discoveryservices@invitrogen.com

Family Receptor Parental Cell Line
Acetylcholine (Muscarinic)M1NFAT-bla CHO-K1
Acetylcholine (Muscarinic)M2U2OS
Acetylcholine (Muscarinic)M3NFAT-bla CHO-K1
Acetylcholine (Muscarinic)M4Gqo5-NFAT-bla CHO-K1
Acetylcholine (Muscarinic)M4U2OS
Acetylcholine (Muscarinic)M5NFAT-bla CHO-K1
AdenosineADORA1U2OS
AdenosineADORA3
U2OS
AdrenoreceptorADRA1ANFAT-bla CHO-K1
AdrenoreceptorADRA1BNFAT-bla CHO-K1
AdrenoreceptorADRA2AGqo5-NFAT-bla CHO-K1
AdrenoreceptorADRA2AU2OS
AdrenoreceptorADRB1CRE-bla CHO-K1
AdrenoreceptorADRB3CRE-bla CHO-K1
AngiotensinAGTR1U2OS
Angiotensin-LikeAGTRL1U2OS
BradykininB1NFAT-bla CHO-K1
BradykininB2
NFAT-bla CHO-K1
CalcitoninCALCRCRE-bla Freestyle 293F
CalcitoninCALCRL:RAMP1CRE-bla Freestyle 293F
CalcitoninCALCRL:RAMP3CRE-bla Freestyle 293F
Calcium SensingCaSRGqo5-NFAT-bla CHO-K1
CannabinoidCNR1Galpha15-NFAT-bla CHO-K1
CannabinoidCNR2U2OS
ChemokineCCR1U2OS
ChemokineCCR2U2OS
ChemokineCCR3U2OS
ChemokineCCR4U2OS
ChemokineCCR5U2OS
ChemokineCCR6U2OS
ChemokineCCR7U2OS
ChemokineCXCR1U2OS
ChemokineCXCR2U2OS
ChemokineCXCR3U2OS
ChemokineCXCR4U2OS
ChemokineCXCR6U2OS
ChemokineCXCR7U2OS
Chemokine LikeCMKLR1U2OS
CholecystokininCCKARNFAT-bla HEK 293T
CholecystokininCCKBRNFAT-bla HEK 293T
Complement ComponentC5AR1Galpha15-NFAT-bla CHO-K1
Corticotropin Releasing FactorCRHR1CRE-bla CHO-K1
Corticotropin Releasing FactorCRHR2CRE-bla CHO-K1
DopamineD1CRE-bla CHO-K1
DopamineD2Gqo5-NFAT-bla CHO-K1
Endothelial Differentiation GeneEDG1U2OS
Endothelial Differentiation GeneEDG2U2OS
Endothelial Differentiation GeneEDG3Galpha15-NFAT-bla HEK 293T
Endothelial Differentiation GeneEDG4U2OS
Endothelial Differentiation GeneEDG6U2OS
Endothelial Differentiation GeneEDG7NFAT-bla HEK 293T
Endothelial Differentiation GeneEDG8U2OS
EndothelinEDNRANFAT-bla HEK 293T
EndothelinEDNRAU2OS
EndothelinEDNRBNFAT-bla HEK 293T
Formyl Peptide-LikeFPRL1Galpha15-NFAT-bla CHO-K1
GalaninGALR1U2OS
GalaninGALR2U2OS
Gastrin Releasgin PeptideGRPRNFAT-bla CHO-K1
Ghrelin
GHSR
U2OS
GlucagonGCGR
CRE-bla CHO-K1
GlucagonGIPR
CRE-bla HEK 293T
GlucagonGLP1RCRE-bla CHO-K1
GlucagonGLP2R
U2OS
Gonadotrophin-Releasing Hormone
GnRHR
NFAT-bla CHO-K1
HistamineH1NFAT-bla HEK 293T
HistamineH2CRE-bla HEK 293T
HistamineH3U2OS
HistamineH4U2OS
KiSS1GPR54NFAT-bla CHO-K1
LeukotrienecysLT2NFAT-bla CHO-K1
LeukotrieneLTB4RGalpha15-NFAT-bla CHO-K1
Melanin-Concentrating HormoneMCHR1 (GPR24)Gqi5-NFAT-bla CHO-K1
Melanin-Concentrating HormoneMCHR2U2OS
Melanin-Concentrating HormoneMCHR2NFAT-bla CHO-K1
MelanocortinMC1RCRE-bla CHO-K1
MelanocortinMC2RCRE-bla CHO-K1
MelanocortinMC3RCRE-bla CHO-K1
MelanocortinMC4RCRE-bla CHO-K1
MelanocortinMC5RCRE-bla CHO-K1
MelatoninMTNR1AU2OS
MelatoninMTNR1BU2OS
Neuromedin UNMUR1NFAT-bla CHO-K1
Neuropeptide SNPSR1-ANFAT-bla CHO-K1
Neuropeptide SNPSR1-BNFAT-bla CHO-K1
Neuropeptide WGPR8U2OS
Neuropeptide YNPY1RU20S
Neuropeptide YNPY2RU2OS
NeurotensinNTSR1NFAT-bla CHO-K1
Nicotinic AcidGPR109AU2OS
OpioidOPRD1U2OS
OpioidOPRK1Gqo5-NFAT-bla CHO-K1
OpioidOPRK1U2OS
OpioidOPRL1Gqi5-NFAT-bla Freestyle 293F
OpioidOPRL1U2OS
OpioidOPRM1U2OS
OrexinHCRTR1NFAT-bla CHO-K1
OrexinHCRTR2Galpha15-NFAT-bla CHO-K1
Orphan
GPR1
U2OS
OprhanGPR119CRE-bla CHO-K1
OprhanGPR35
U2OS
P2YP2RY11NFAT-bla CHO-K1
P2YP2RY2NFAT-bla CHO-K1
P2YP2RY6NFAT-bla CHO-K1
Platelet Activating FactorPTAFRU2OS
Prolactin Releasing PeptideGPR10NFAT-bla CHO-K1
Prostanoid
GPR44 (CRTH2)
U2OS
ProstanoidPTGDRCRE-bla CHO-K1
ProstanoidPTGER1
NFAT-bla CHO-K1
ProstanoidPTGER2CRE-bla CHO-K1
ProstanoidPTGIR
CRE-bla CHO-K1
ProstanoidTBXA2RU2OS
Protease ActivatedF2LR1U2OS
RelaxinRLN3R1U2OS
SecretinSCTRCRE-bla CHO-K1
SerotoninHTR1AGalpha15-NFAT-bla CHO-K1
SerotoninHTR1AU2OS
SerotoninHTR2AU2OS
SerotoninHTR2B
NFAT-bla CHO-K1
SerotoninHTR7CRE-bla CHO-K1
SomatostatinSSTR1U2OS
SomatostatinSSTR2U2OS
SomatostatinSSTR5U2OS
TachykininTACR1U2OS
TachykininTACR2NFAT-bla CHO-K1
TachykininTACR3U2OS
Vasopressin & Oxytocin
AVPR1ANFAT-bla CHO-K1
Vasopressin & Oxytocin
AVPR2CRE-bla CHO-K1
Vasopressin & OxytocinOXTR
Gqo5-NFAT-bla CHO-K1
VIP & PACAPPAC1CRE-bla CHO-K1
VIP & PACAPVPAC1CRE-bla CHO-K1
VIP & PACAPVPAC2CRE-bla CHO-K1

For Research Use Only. Not for use in diagnostic procedures.