Commercial-Use Immunoreagents

Life Technologies offers a broad portfolio of products and technologies for immunodetection, including antibodies, secondary detection reagents, and immunoassay kits. These tools and their associated intellectual property are available for your use in developing and offering products and services for sale. Our reagents are employed by our commercial-use customers in research and diagnostic services and are resold by those companies in kits with multiple formats including ELISAs, bead-based immunoassays, western blots, immunoprecipitation, automated imaging, immunocytochemistry, immunohistochemistry, automated immunoassay instruments, lateral flow immunoassays, and flow cytometry.  

Antibodies and Secondary Detection

Primary Antibodies
Life Technologies has a diverse collection of thousands of highly specific RUO, ASR, and IVD primary antibodies to targets for use in clinical and research settings. These antibodies are validated using a combination of tests, including western blotting, immunocytochemistry, immunohistochemistry, ELISAs, and flow cytometry, depending on the target application.

Our portfolio includes antibodies directed against CD markers, cancer markers, immunology targets, stem cell markers, cell signaling targets, cytokines and chemokines, cell organelles and cell structure targets, cell junctions, and adhesion molecules, as well as other key targets involved in a variety of cellular processes.

When you use Life Technologies antibodies in your commercial services and kits, you get:

  • Confidence—extensive validation allows for highly specific antibodies
  • Performance—robust antibodies validated for multiple applications
  • Selection—large menu of unique specificities

Phosphospecific Antibodies
Life Technologies offers more than 300 phosphorylation site–specific antibodies (phosphospecific antibodies) and is actively expanding this product line to enable analysis of key targets in normal and disease states including cardiovascular disease, cancer, inflammation, neurodegenerative diseases, and diabetes. These new tools are providing critical insight into the investigation of complex signal transduction events including unraveling the intricacies of drug intervention in disease. These antibodies are available for use in your commercial drug discovery research services, your human diagnostics service offering, or for inclusion in a kit or other product for resale.

Flow Cytometry Antibodies
Life Technologies has a wide selection of antibodies that are validated for flow cytometry, including CD markers, antibodies for cancer, immunology, stem cell studies, cell signaling, cytokines, and chemokines, as well as for other key targets involved in a variety of cellular processes, plus much more. If you are building antibody cocktails for flow cytometry applications, you can find the specific antibodies you need and appropriate color combinations at Antibodies for Flow Cytometry.

Secondary Antibodies
Our Alexa Fluor® secondary antibody conjugates and streptavidin conjugates set the standard for detection in the life science industry.  Life Technologies’ anti-GFP antibody is the most commonly used secondary detection reagent for fluorescent proteins in fixed samples. We offer anti-dye, anti-hapten, and anti-tag antibodies that permit multicolor signal amplification in essentially any immunoassay format. To identify the best secondary antibody for your application, please try our secondary antibody selector tool.

Our Alexa Fluor® Zenon® kits permit you to combine multiple primary antibodies of the same species in the same sample, without having to prepare conjugates. Up to seven colors of Zenon® reagents have been used at the same time to detect samples in pathology assays and flow cytometry. 

Enzyme Substrates
Life Technologies provides a complete selection of secondary detection reagents for use in signal amplification. Build your immunoassay kit or service using Tropix® chemiluminescence substrates, Amplex® Red fluorogenic horseradish peroxidase substrates, or standard colorimetric substrates.

Another very powerful enzymatic secondary detection technology is based on Tyramide Signal Amplification (TSA®). TSA® (PerkinElmer) is an enzyme-mediated detection method that utilizes the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid sequence in situ. We can custom-formulate these reagents and specially package them for direct inclusion in your kits or for use in your service.

More detailed information about our antibodies and secondary detection technologies is available on our product web pages.

Conjugates
We offer 24 different fluorescent labels for primary antibodies, secondary antibodies, anti-dye and anti-hapten antibodies, and streptavidin, including proprietary labels such as Alexa Fluor®, Texas Red®-X, and Pacific Blue™ dyes, which span the visible light spectrum from deep blue to near-infrared emission, generic fluorophores such as fluorescein and tetramethylrhodamine, R-phycoerythrin (RPE), allophycocyanin (APC), and PerCP. We also offer conjugates of biotin, DNP, digoxigenin, dansyl, and other haptens. We have more than 30 years experience in preparing custom antibody conjugates to match your instrument and optical specifications, which are optimized to meet your application-specific assay needs. We can also couple antibodies to gold particles, polystyrene microspheres and nanospheres, and magnetic particles for you.
Learn More about Microspheres and Nanocrystals (link to Microspheres OEM web page)

We are also highly experienced with conjugating antibodies and streptavidin to enzymes including alkaline phosphatase, horseradish peroxidase, beta-galactosidase, and others. Please contact us at outlicensing@lifetech.com for more information.

Immunoassays

ELISA, phosphoELISA™, and Luminex® Assay Kits
To quantitate specific protein levels with high specificity and sensitivity in a wide variety of sample types, we carry a broad range of commercial-use immunoassays and immunoassay reagents, using antibody pairs for single-analyte (ELISA and phosphoELISA™ kits) as well as multi-analyte (Luminex® Assays) analysis. Multiplexing allows for simultaneous analysis of multiple markers in the same sample, saving time, money, and sample.

The kits are convenient and ready to use for measuring cytokine secretion and the associated downstream phosphorylated proteins to get the complete story both inside and outside the cell.

Build Your Own Immunoassay

From prequalified antibody pairs to buffers and detection reagents, we provide everything you need to develop and build your own immunoassay for use in your products or services.

Protein Detection and Quantitation

TC-FlAsH™ Expression Analysis Detection Kits

  • Rapid, direct labeling of purified proteins or crude cell lysate
  • Visualize your protein of interest directly in the gel and even within the cassette, alongside a reference Benchmark™ protein ladder, using a contrasting stain for total protein detection

TC-FlAsH™ technology allows you or your customers to add a small tetracysteine tag to target proteins, which can then be isolated or detected rapidly in living cells, in vitro in solution, and in gels.  This method is complementary to immunodetection methods, and does not require the use of an antibody. Licenses are available for use of the technology in contract research, and for inclusion of tetracysteine-labeled proteins in kits for resale, along with your choice of detection or isolation reagents.

Click-iT® Detection of Protein Synthesis & Posttranslational Modification

Click chemistry technology provides a novel, bio-orthogonal method for linking together any two ligands through the use of a special catalyst. We have developed reagents for using this powerful technology to label nascent DNA, RNA, and proteins in vivo or in vitro. In addition, we have developed a portfolio of posttranslational modification kits and tools to allow you to characterize proteins. These tools are available, under license, for use in your services or for inclusion in kits.

  • Open the door to a radioactivity-free lab
  • Click chemistry detects key posttranslational modifications (PTM)
  • Data delivery in hours to days
  • Experience the ultimate in flexible detection—with cells, gels, blots, or even mass spectrometry analyses
  • Learn More about Click-iT® Detection Assays

Western Blot Detection Kits

The future of western blots has never been brighter with tools available from Life Technologies. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) are two of the most popular reporter enzymes currently used in western blot detection. Life Technologies offers both AP-based and HRP-based kits for chemiluminescent, chromogenic, and fluorescent detection of proteins with western blotting.

Protein Quantitation

We have a broad selection of protein quantitation reagents for measurement of proteins in solution.  These include fluorogenic and colorimetric kits and reagents. Please contact us to learn more about the best technology for use in your kit or service.

Protein Gel Electrophoresis

Separate proteins easily using NuPAGE®, Novex®, and NativePAGE™ gels and optimized stains and standards for fast and accurate electrophoresis results.

 

Biomarker Discovery

If you are offering biomarker discovery services or developing kits for that purpose, you may be interested in our innovative ProtoArray® protein microarrays, mass spectrometry labeling reagents, Dynabeads® magnetic particles, and the ZOOM® Benchtop Proteomics™ System, as well as our Alexa Fluor®  secondary antibodies, which set the standard for marker detection via high-content screening/automated imaging applications. Our protein electrophoresis portfolio can be used in combination with 1D or 2D gels (link to Electrophoresis Tools OEM web page) for comparing relative abundance and detecting modifications such as phosphorylation, glycosylation, or lipid modification.

Protein–Protein Interactions
If you are interested in offering kits or services to study protein–protein interactions using native proteins and protein complexes, Life Technologies offers the ProQuest™ Two-Hybrid System, ProtoArray® protein microarrays, and the NativePAGE™ gel system. You can also isolate large, intact protein complexes using Dynabeads® magnetic particles.

For more information, please contact outlicensing@lifetech.com.