Probe Labeling Systems
Amino Allyl Labeling for Array Analysis
Description: This short article discusses the differences between direct incorporation and post-synthesis reaction methods for fluorescently labeling nucleic acids for array analysis.
BrightStar™ Psoralen-Biotin Labeled Probes - Technical Bulletin #175
Does It Make Antisense?
Description: A basic definition of coding, noncoding, antisense, and template DNA strands.
In Vitro Transcription: The Basics
Description: This article presents an overview of in vitro transcription, including requirements of in vitro transcription reactions and a comparison of conventional vs. large scale RNA synthesis. This is a good place to start if you haven't performed in vitro transcription in the past and want to know what's involved.
In Vitro Synthesis of RNA - Technical Bulletin #156
Phage Polymerase Promoters: Minimal Sequence Requirements
Description: T3, T7 and SP6 RNA polymerase consensus promoter sequences.
Practical Tips for In Vitro Transcription - Technical Bulletin #182
Practical Tips for Nonisotopic Detection - Technical Bulletin #184
Probe Labeling Chart
Description: This handy chart lists probes by type (RNA, cDNA, ssDNA, dsDNA, oligonucleotide) and lists applications, starting material, probe synthesis reaction, and the Ambion Kit we recommend.
Probe Specific Activity - Technical Bulletin #162
When Labeled Nucleotides Go Bad - Technical Bulletin #170
Description: This short article describes an experiment to determine if your labeled nucleotide has "gone bad."
Description: A protocol for monitoring the efficiency of radiolabeled nucleotide incorporation in a polymerization reaction by TCA precipitation.
Description: An overview of Ambion's complete line of products for the generation of capped/uncapped RNA, short RNA transcripts, dsRNA transcripts, amplified RNA, and milligram quantities of transcript.
New Tools for Small RNA Analysis - TechNotes 11(1)
Description: An overview of Ambion's products for the study of small RNAs. Includes the mirVana™ products for the isolation and detection of small RNAs.
Prepare siRNA and miRNA Probes in Just 1 Hour - TechNotes 10(4)
Description: Introducing our new mirVana™ miRNA Probe Construction Kit.
Rapidly Prepare Probes and Markers for Small RNA Analysis - TechNotes 11(2)
Description: The new mirVana™ Probe & Marker Kit provides a complete solution to quickly prepare and purify high specific activity radiolabeled RNA probes and small RNA markers.
Recombinant HIV Reverse Transcriptase - TechNotes 11(1)
Description: Ambion now offers a new reverse transcriptase that is more thermostable than M-MLV and AMV making it the superior choice for troublesome cDNA synthesis reactions.