Description: Here, we review three popular RNA detection/quantitation methods: Northern blot analysis, Ribonuclease Protection Assays (RPAs) and Reverse Transcription Polymerase Chain Reaction (RT-PCR). All of these can be used to confirm results from microarray analysis and differential display experiments.
Comparison of Different Methods of mRNA Quantitation
Description: A brief comparison of nuclease protection assays, Northern blotting, RT-PCR and in situ hybridization for the detection and quantitation of RNA.
Getting Rid of Residual Full Length Probe in Ribonuclease Protection Assays
Description: Tips on how to prevent template DNA from contaminating your RPA probe.
Hang Up in the Wells - Preventing Samples from Remaining in the Wells of Your Gels - Technical Bulletin #163
Linearity of RNase Protection Assays - Technical Bulletin #501
Description: The experiment described in this Technical Bulletin demonstrates the use of Ambion's RPA III™ Kit to quantitate a target mRNA with a high degree of precision. It shows that this technique is capable of accurately detecting differences in target mRNA abundance that vary by as little as 20%.
Nuclease Protection Assays: The Basics
Description: "Nuclease Protection Assays: The Basics" explains the advantages and limitations of nuclease protection assays (NPAs), as well as the steps involved. If you have never performed an NPA, or are confused about which NPA kit to choose, this article is for you.
Optimal Probe Concentrations for Ambion's Ribonuclease Protection Assay Kits - Technical Bulletin #167
Simultaneous Detection of Multiple mRNA Targets with Ribonuclease Protection Assays - Technical Bulletin #155