Organisms Compatible with the MICROBExpress™ mRNA Isolation Kit

Ambion has thoroughly searched 16S rRNA and 23S rRNA sequences from published genome sequences and from various databases and has identified organisms that should be compatible with the MICROBExpress Kit. Ambion has also identified organisms that are partially compatible with MICROBExpress and those that are incompatible with MICROBExpress.

Information regarding compatibility of some bacterial species whose genome sequences are in progress may not be available at this time.

Some bacterial species process the 23S rRNA into smaller fragments (usually two). Although the sequence of the capture oligonucleotides used in MICROBExpress may be compatible with these species, the MICROBExpress Kit may not function to completely remove shortened fragments of processed 23S rRNA.

The following organisms should be compatible with MICROBExpress.


  • Acidithiobacillus ferrooxidans
  • Acinetobacter calcoaceticus
  • Actinobacillus actinomycetemcomitans
  • Aeromonas hydrophila
  • Agrobacterium tumefaciens
  • Alcaligenes faecalis
  • Bacillus alcalophilus
  • Bacillus anthracis
  • Bacillus cereus
  • Bacillus halodurans
  • Bacillus licheniformis
  • Bacillus mycoides
  • Bacillus subtilis
  • Bacillus thuringiensis
  • Bartonella bacilliformis
  • Bordetella avium
  • Bordetella bronchiseptica
  • Bordetella parapertussis
  • Bordetella pertussis
  • Borrelia burgdorferi
  • Bradyrhizobium japonicum
  • Bradyrhizobium lupini
  • Brevundimonas diminunata
  • Brucella melitensis
  • Brucella melitensis biovar suis
  • Buchnera aphidicola
  • Buchnera sp. APS
  • Burkholderia cepacia
  • Burkholderia mallei
  • Burkholderia pseudomallei
  • Campylobacter spp. (with module)
  • Caulobacter crescentus
  • Chlamydia muridarum
  • Chlamydia suis
  • Chlamydia trachomatis
  • Chlamydophila abortus
  • Chlamydophila caviae
  • Chlamydophila felis
  • Chlamydophila pecorum
  • Chlamydophila pneumoniae
  • Chlamydophila psittaci
  • Chlorobium limicola
  • Chlorobium tepidum
  • Citrobacter freundii
  • Clostridium acetobutylicum
  • Clostridium difficile
  • Clostridium histolyticum
  • Corynebacterium diptheriae
  • Corynebacterium glutamicum
  • Cyanobacteria (with module)
  • Cytophaga hutchisonii
  • Desulfovibrio vulgaris
  • Dichelobacter nodosus
  • Enterococcus asini
  • Enterococcus avium
  • Enterococcus casseliflavus
  • Enterococcus cecorum
  • Enterococcus columbae
  • Enterococcus dispar
  • Enterococcus durans
  • Enterococcus faecalis
  • Enterococcus faecium
  • Enterococcus flavescens
  • Enterococcus gallinarum
  • Enterococcus hirae
  • Enterococcus malodoratus
  • Enterococcus mundtii
  • Enterococcus pseudoavium
  • Enterococcus raffinosus
  • Enterococcus saccharolyticus
  • Enterococcus sulfureus
  • Erwinia chrysanthemi
  • Escherichia coli
  • Fibrobacter succinogenes
  • Frankia sp.
  • Fusobacterium nucleatum
  • Geobacillus stearothermophilus
  • Geobacter sulfurreducens
  • Gluconacetobacter europaeus
  • Gluconacetobacter intermedius
  • Gluconacetobacter xylinus
  • Haemophilus ducreyi
  • Haemophilus influenzae
  • Helicobacter pylori (with module)
  • Klebsiella pneumoniae
  • Lactobacillus amylolyticus
  • Lactobacillus delbrueckii
  • Lactococcus lactis
  • Leuconostoc carnosum
  • Leuconostoc lactis
  • Leuconostoc mesenteroides
  • Listeria grayi
  • Listeria innocua
  • Listeria ivanovii
  • Listeria monocytogenes
  • Listeria seeligeri
  • Melissococcus plutonius
  • Micrococcus luteus
  • Mycobacterium avium
  • Mycobacterium avium supsp. paratuberculosis
  • Mycobacterium bovis
  • Mycobacterium kansasii
  • Mycobacterium leprae
  • Mycobacterium phlei
  • Mycobacterium smegmatis
  • Mycobacterium tuberculosis
  • Myxococcus xanthus
  • Neisseria gonorrhoeae
  • Neisseria meningitidis
  • Nitrosomonas europaea
  • Pasteurella multocida
  • Peptococcus niger
  • Plesiomonas shigelloides
  • Pseudomonas aeruginosa (with module)
  • Pseudomonas putida (with module)
  • Pseudomonas syringae (with module)
  • Ralstonia pickettii
  • Ralstonia solanacearum
  • Renibacterium salmoninarum
  • Rhizobium vitis
  • Rhodobacter (with module)
  • Rhodococcus erythropolis
  • Rhodococcus fascians
  • Rhodopseudomonas palustris
  • Rhodospirillum rubrum
  • Rickettsia akari
  • Rickettsia australis
  • Rickettsia bellii
  • Rickettsia canadensis
  • Rickettsia conorii
  • Rickettsia montanensis
  • Rickettsia parkeri
  • Rickettsia prowazekii
  • Rickettsia rhipicephali
  • Rickettsia rickettsii
  • Rickettsia sibirica
  • Rickettsia typhi
  • Salmonella bongori
  • Salmonella enterica
  • Salmonella enteritidis
  • Salmonella paratyphi
  • Salmonella typhi
  • Salmonella typhimurium
  • Shewanella putrefaciens
  • Sinorhizobium meliloti
  • Sporosarcina globispora
  • Staphylococcus aureus
  • Staphylococcus carnosus
  • Staphylococcus condimenti
  • Staphylococcus epidermidis
  • Stigmatella aurantiaca
  • Streptococcus equii
  • Streptococcus gordonii
  • Streptococcus macedonicus
  • Streptococcus mitis
  • Streptococcus mutans
  • Streptococcus oralis
  • Streptococcus parauberis
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Streptococcus thermophilus
  • Streptococcus uberis
  • Streptomyces ambofaciens
  • Streptomyces coelicolor
  • Streptomyces griseus
  • Streptomyces lividans
  • Streptomyces nodosus
  • Streptomyces rimosus
  • Thermoanaerobacter tengcongensis
  • Thermobifida fusca
  • Thermomonospora chromogena
  • Thiobacillus ferrooxidans
  • Trteponema denticola
  • Treponema pallidum
  • Vibrio cholerae
  • Yersinia enterocolitica
  • Yersinia pestis
  • Xanthomonas campestris
  • Xanthomonas axonopodis pv. citri
  • Xylella fastidiosa

The following organisms should be partially compatible with MICROBExpress


Compatible with removal of 23S rRNA and 50-100% of 16S rRNA:

  • Azotobacter vinelandii
  • Bacteroides fragilis
  • Carboxydothermus hydrogenoformans
  • Clostridium tyrobutyricum
  • Desulfovibrio vulgaris
  • Dictyoglomus thermophilum
  • Enterococcus solitarius
  • Erysipelothrix rhusiopathiae
  • Erysipelothrix tonsillarum
  • Flexibacter flexilis
  • Legionella pneumophila
  • Leptospira interrogans
  • Leucothrix mucor
  • Listeria welshimeri
  • Methylococcus capsulatus
  • Myroides odoratus
  • Oenococcus oeni
  • Paracoccus denitrificans
  • Pectinatus frisingensis
  • Porphyromonas gingivalis
  • Prevotella intermedia
  • Silicibacter pomeroyi
  • Tannerella forsythensis
  • Tetragenococcus halophilus
  • Thermobispora bispora
  • Thermus thermophilus
  • Thiomonas cuprina

Compatible with removal of 16S rRNA and 50-100% of 23S rRNA:

  • Burkholderia fungorum
  • Clostridium perfringens
  • Desulfitobacterium hafniense
  • Magnetospirillum magnetotacticum
  • Mesorhizobium loti
  • Nannocystis exedens
  • Novosphingobium aromaticivorans
  • Parachlamydia acanthamoebae
  • Ruminobacter amylophilus
  • Ruminococcus albus
  • Tropheryma whipplei
  • Wolbachia endosymbiont of Drosophila melanogaster

The following organisms are NOT compatible with MICROBExpress

  • Archaebacteria
  • Chloroflexus aurantiacus
  • Dehalococcoides ethenogenes
  • Deinococcus radiodurans
  • Fervidobacterium islandicum
  • Mycoplasma spp.
  • Pirellula marina
  • Propionibacterium freundenreichii
  • Simkania negevensis
  • Thermotoga maritima
  • Ureaplasma urealyticum
 

Ordering Information

Sku Name Size Price Qty
AM1905 MICROBExpress™ Bacterial mRNA Enrichment Kit 20 preps USD 401.00