RNAlater® Solution can be used to stabilize and protect cellular RNA in intact unfrozen samples through rapid penetration of fresh tissue and deactivation of nucleases. Dr. Gorokhova (Stockholm University, Sweden) designed an experiment to determine the amount of time a microcrustacean specimen can be stored in RNAlater Solution as an alternative to deep freezing of the sample. The study demonstrated that RNAlater Solution successfully preserved both RNA and DNA for up to one month at room temperature, and for up to four months at 5°C.
Preserving Aquatic Samples in the Field
Total cellular RNA and DNA were quantified in individual nauplii using a fluorometric assay. RNA and DNA yields were subsequently compared with control samples (fresh animals assayed immediately after hatching) and among preservation treatments to determine the longterm effects of different sample storage techniques. Nucleic acid quantities were determined for each preservation treatment after one, two, four, and eight months. For both RNA and DNA yields, shrimp samples stored in RNAlater Solution at 5°C were equivalent to those stored at -80°C for at least four months after preservation.
The study concluded that RNAlater Solution successfully preserved both RNA and DNA for up to one month at room temperature, and up to four months at 5°C, suggesting that RNAlater Solution provides reliable sample preservation immediately upon collection. This result demonstrates that use of RNAlater Solution for extended storage is equal to the established gold-standard of storage at -80°C. Also, with small organisms RNAlater Solution provides the flexibility of storing them intact.
*nauplii is a developmental stage in brine shrimp.
- Gorokhova E (2005) Effects of preservation and storage of microcrustaceans in RNAlater on RNA and DNA degradation, Limnol. Oceanogr Methods 3:143–148.