A number of different methods, technologies, and protocols are available for performing the actual cloning reaction. To choose the one that best meets your needs, you will need to analyze the current parameters of your experiment and plan ahead for any downstream procedures. This section discusses several highly efficient cloning technologies:

The table below can help you navigate your way through these technologies.

  Restriction Enzyme Cloning TA-Cloning TOPO® (TA, blunt, directional) Gateway® GeneArt® Seamless Cloning GeneArt® Type IIs Assembly GeneArt® High-Order Genetic Assembly
Fragments Cloned Simultaneously 1 1 1 Up to 4 Up to 4 Up to 8 Up to 10
Max Fragment (s) Size Variable 1-3 Kbp <5 kbp
(<10 Kbp for XL-TOPO®)
Variable Up to 10 Kbp, max total size of 13 Kbp Up to 10 Kbp, max total size of 13 Kbp Up to 100 Kbp, max total size of 110 Kbp
Gene Shuttling Between Vectors w/o PCR or Restriction Enzymes NO NO NO YES NO NO NO
Seamless (No Extra Sequences) NO NO NO NO YES YES YES
Use Your Own Vector YES NO NO YES (may require conversion)
YES YES YES
Time to Clone Multiple Fragments Days to Weeks Not Possible Not
Possible
>4 Days 1 hr 1 hr 3 Days
4 Fragment Cloning Efficiency NA NA NA 30% - 85% 75% >90% >90%
Web-Based Vector Design Tool NO NO NO NO YES YES YES

Browse for vectors using our Vector selection tool