Step 1:  Obtain genomic starting material

No matter what starting source is available, you will need genomic material to begin the cloning process (Figure 1).

Obtaining Genomic Starting Material

There are various ways to prepare or obtain ready-to-clone DNA, including:

 1.  Purchasing a Premade Clone

2.  Obtaining Clones from a cDNA Library

3.  Purifying DNA from Tissues or Cells
4.  Reverse-Transcribe RNA

5.  Amplifying DNA
 


Select from each of these methods to learn more about how to obtain DNA using these methodologies.

Figure 1:  Cloning workflow