No matter what the source of DNA, you will most likely need to use PCR to generate sufficient material for cloning. We offer best in-class PCR enzymes with brands you trust including our most popular enzymes for cloning (Table 1).

Hot-start Amplification of a Broad Range of Targets

AmpliTaq Gold® 360 DNA Polymerase (Roche) and Platinum® Taq DNA Polymerase

  • Increased specificity with choice of trusted hot-start technologies
  • Amplify the broadest range of targets (<3 kb)
  • Available in Master Mix or SuperMix formats for increased convenience

Chemically modified hot-start AmpliTaq Gold® 360 DNA Polymerase has been optimized to amplify a wide range of targets while delivering the high specificity, yield, and reliability you have come to expect.

Platinum® Taq DNA Polymerase uses antibody-mediated hot-start to enable increased specificity, yield, and sensitivity with the fastest activation times. Although there are several types of hot-start enzymes, the most reliable and widely used techniques involve chemically modified hot-start or antibody-mediated hot-start enzymes.

How to Choose Between Chemically Modified & Antibody-mediated Hot-start Reagents

Chemically modified hot-start reagents are best when performing high-throughput applications. These enzymes withstand longer periods at room temperature. However, the activation time is longer and the read lengths are shorter. Antibody-mediated hot-start products deliver fast activation times and minimal template degradation, which promote the amplification of longer fragments. However, antibody-mediated chemistries tend to exhibit reduced specificity and involve longer setup times.

With both types of hot-start technologies available from Life Technologies, you can choose the chemistry that works best for your research.

Robust PCR Aplification with High Yields & Increased Fidelity

Platinum® Taq DNA Polymerase High Fidelity

  • Greater than six times higher fidelity than Taq DNA polymerase
  • Amplification of fragments up to 20 kb
  • High yields with no need to optimize reaction conditions

The accuracy of Platinum® Taq DNA Polymerase High Fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´ > 5´ exonuclease activity) enzyme from Pyrococcus species, GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic “hot-start” technology. Platinum® Taq DNA Polymerase High Fidelity is especially effective for high-fidelity applications such as cloning or mutagenesis.  Platinum® Taq DNA Polymerase High Fidelity predominantly produces PCR products with 3’-A overhangs for compatibility with TOPO® TA Cloning® Kits.

Ordering Information

Sku Name Size Price Qty
4398813 AmpliTaq Gold® 360 DNA Polymerase 100 units USD 95.00
4398823 AmpliTaq Gold® 360 DNA Polymerase 250 units USD 238.00
4398876 AmpliTaq Gold® 360 Master Mix 1 mL USD 112.00
4398881 AmpliTaq Gold® 360 Master Mix 5 mL USD 260.00
10966026 Platinum® Taq DNA Polymerase 300 reactions USD 258.00
10966034 Platinum® Taq DNA Polymerase 600 reactions USD 459.00
11306016 Platinum® PCR SuperMix 100 reactions USD 140.00
11304011 Platinum® Taq DNA Polymerase High Fidelity 100 reactions USD 165.00
11304029 Platinum® Taq DNA Polymerase High Fidelity 500 reactions USD 770.00
12532016 Platinum® PCR SuperMix High Fidelity 100 reactions USD 237.00
12346086 AccuPrime™ Taq DNA Polymerase, high fidelity 200 reactions USD 331.00
12346094 AccuPrime™ Taq DNA Polymerase, high fidelity 1,000 reactions USD 1,490.00
18427013 10 mM dNTP Mix 100 µL USD 81.00
10297018 100 mM dNTP Set 4 x 250 µL USD 259.00

Which Standard PCR Enzyme Is Right for You?

  Value Choice for non-Hot Start DNA Polymerase Value Choice for non-Hot Start DNA Polymerase General Choice for Hot Start DNA Polymerase Premium Choice for Hot Start DNA Polymerase
Top Selleryesyes
Improved Specificityyesyes
Improved Sensitivityyes
Amplicon Size Up to 5 kb Up to 5 kb Up to 5 kb Up to 8 kb
Improved Yield yes
GC-Rich Formatyes yes
Activation Time Immediate Immediate 10 minutes 30 seconds
Master Mix/Super Mix Format yes yesyesyes
Price/Reaction$0.30 - $0.55 $0.44 - $0.55 $0.69
(4,000 Reactions)
$0.72
(5,000 Reactions)
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