Purifying your PCR Product
Begin your cloning with only the PCR products you want:
Some cloning methods, such as TOPO® Cloning, allow you to go directly from PCR to clone without any additional purification steps. For those applications that require PCR clean-up or validation of PCR results, there are two methods generally followed: PCR product isolation using a column, and gel purification from an agarose gel.
For Quick & Easy Gel Extraction or PCR Purification of DNA Samples
- Purify DNA fragments from 40 bp to 10 kb in <30 min
- High recovery due to high binding column capacity of up to 15 μg of DNA
- Works well with both vacuum and centrifugation protocols
PureLink™ Quick Gel Extraction and PCR Purification Combo Kits offer the ability to perform both a gel extraction or a PCR purification a single kit. The PureLink™ Quick Gel Extraction and PCR Purification Combo Kit is designed to purify DNA fragments from agarose gels. The simple procedure uses a silica-based spin cartridge to purify DNA fragments from 40 bp to 10kb in <30 minutes from TAE or TBE agarose of various percentages and melting points.
The PCR purification protocol achieves rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR fragments >100 bp, typically in less than 10 minutes. DNA isolated using the PureLink™ kit is free of proteins, dye, and agarose, and is ready to use for a variety of applications, including automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling. If you simply require a kit for gel extraction, the PureLink™ Gel Extraction Kit (Figure 1) is designed for fast purification of DNA fragments from TAE or TBE agarose gels of various percentages without the use of organic solvents.
Figure 1: The PureLink™ Gel Extraction Kit procedure is quick and easy. Gel fragments are first solubilized to release the DNA. The sample is then loaded on a PureLink™ spin column and isolated using a simple bind/wash/elute procedure. DNA fragments are recovered in TE buffer or water, ready to use in downstream applications.
Purify PCR Products from a Complex Mixture of DNA Using Silica-based Membrane Technology
- Purify fragments between 100 bp and 15 kb or remove byproducts <300 bp
- Simple bind/wash/elute procedure
- Elute ready-to-use, pure PCR product in typically <10 min
PureLink™ PCR Purification Kits are designed to provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR reactions. Specialized binding buffers allow for purification of PCR products between 100 bp and 15 kb or removal of by-products <300 bp. PCR products purified using the PureLink™ PCR Purification Kits demonstrate outstanding performance in downstream applications such as automated fluorescent DNA sequencing, PCR, restriction mapping, and labeling.
Making your own agarose gels?
- For high-quality agarose gels that resolve DNA and RNA fragments from 100 bp to >30 kb, try our UltraPure™ Agarose