The Gibco® Cell Culture Basics handbook and videos can help your lab attain reproducible results every day. Cell culture is critical to your success, so make sure everyone in your lab uses the best procedures and products to consistently get the outcomes you expect. Cell Culture Basics is an introduction to cell culture, covering topics such as laboratory set-up, safety and aseptic technique. You'll also find basic methods for passaging, freezing, and thawing cultured cells.
Table of Contents
Cell culture webinars
Understanding The In Vitro System and Factors That Affect It
Cultured animal cells do not fully represent the in vivo situation because cells are removed from their natural context and grown in/on an unnatural environment. The goal in culture is to provide a suitable environment which includes appropriate interactions, proper nutrition, and correct signaling. Here we will learn about some of the key factors and culture conditions necessary for animal cell growth and proliferation.
Media Use and Care
Animal cell culture media is a complex mixture and must provide organic and inorganic nutrients at the right levels for a specific cell type. Learn what components are necessary for growth of your cells and why they are included in various formulations. You will also learn that some components, which are critical for cell survival, have limitations and are unstable. We will also discuss differences in content and use, between serum-containing and serum-free media.
Contamination and Prevention
Good cell culture practices dictate that animal cells be grown without contamination by advantageous agents. Hopefully good technique, and not the use of antibiotics contribute to successful culture but sometimes things can go wrong. We will learn what types of contamination are prevalent and what are the best ways to prevent or cure a contamination.
Cell Culture Best Practices
By default, we select for cells with a growth advantage when we passage cells. This, for example results in a bias against slower growing cells. And this results in a change in the cell population. Standard practices should be in place that will limit variability within a cell line, provide appropriate documentation and cell line verification. In this section we will learn how following cell culture best practices will improve your reproducibility and reliability when culturing cells.
Gibco® Cell Culture Companion
Capture and store vital experiment information on your iPhone or iPad in the lab as you count cells, perform cell culture calculations and generate growth curves. And when you're finished, you can export all data files to your Mac or PC.