Basic Laboratory Equipment & Supplies
Video: Introduction to cell culture
This video provides an overview of the basic equipment used in cell culture and proper laboratory set-up. Guidance on how to work safely and aseptically in a cell culture hood is introduced and demonstrated.
Types of Incubators
There are two basic types of incubators, dry incubators and humid CO2 incubators. Dry incubators are more economical, but require the cell cultures to be incubated in sealed flasks to prevent evaporation. Placing a water dish in a dry incubator can provide some humidity, but they do not allow precise control of atmospheric conditions in the incubator. Humid CO2 incubators are more expensive, but allow superior control of culture conditions. They can be used to incubate cells cultured in Petri dishes or multiwell plates, which require a controlled atmosphere of high humidity and increased CO2 tension.
A cell culture laboratory should have storage areas for liquids such as media and reagents, for chemicals such as drugs and antibiotics, for consumables such as disposable pipettes, culture vessels, and gloves, for glassware such as media bottles and glass pipettes, for specialized equipment, and for tissues and cells. Glassware, plastics, and specilized equipment can be stored at ambient temperature on shelves and in drawers; however, it is important to store all media, reagents, and chemicals according to the instructions on the label.
Some media, reagents, and chemicals are sensitive to light; while their normal laboratory use under lighted conditions is tolerated, they should be stored in the dark or wrapped in aluminum foil when not in use.
There are two main types of liquid-nitrogen storage systems, vapor phase and liquid phase, which come as wide-necked or narrow-necked storage containers. Vapor phase systems minimize the risk of explosion with cryostorage tubes, and are required for storing biohazardous materials, while the liquid phase systems usually have longer static holding times, and are therefore more economical.
Narrow-necked containers have a slower nitrogen evaporation rate and are more economical, but wide-necked containers allow easier access and have a larger storage capacity.
A cell counter is essential for quantitative growth kinetics, and a great advantage when more than two or three cell lines are cultured in the laboratory.
The Countess® Automated Cell Counter is a benchtop instrument designed to measure cell count and viability (live, dead, and total cells) accurately and precisely in less than a minute per sample, using the standard Trypan Blue uptake technique. Using the same amount of sample that you currently use with the hemocytometer, the Countess® Automated Cell Counter takes less than a minute per sample for a typical cell count and is compatible with a wide variety of eukaryotic cells.