Useful Information for Various Sizes of Cell Culture Dishes and Flasks

There are various sizes of dishes and flasks used for cell culture. Some useful numbers such as surface area and volumes of dissociation solutions are given below for various size culture vessels.

Surface Area (cm2) Seeding Density Cells at Confluency1 Versene (ml of 0.05% EDTA) Trypsin (ml of 0.05% tryspin, 0.53 mM EDTA) Growth Medium (ml)
Dishes
35mm 9 0.3 x 106 1.2 x 106 1 1   2
60mm 21 0.8 x 106 3.2 x 106 3 2 3
100mm 55 2.2 x 106 8.8 x 106 5 3 10
150mm 152 5.0 x 106 20.0 x 106 10 8 20
Culture Plates
6-well 9 0.3 x 106 1.2 x 106 2 2   3 - 5
12-well 4 0.1 x 106 0.4 x 106 1 1 1- 2
24-well 2 0.05 x 106 0.2 x 106 0.5 0.5 0.5 - 1.0
Flasks
T-25 25 0.7 x 106 2.8 x 106 3 3 3 - 5
T-75 75 2.1 x 106 8.4 x 106 5 5 8 - 15
T-160 162 4.6 x 106 18.4 x 106 10 10 15 - 30

1The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, HeLa cells were used.