Useful Information for Various Sizes of Cell Culture Dishes and Flasks

There are various sizes of dishes and flasks used for cell culture. Some useful numbers such as surface area and volumes of dissociation solutions are given below for various size culture vessels.

 Surface Area (cm^2)
Seeding DensityCells at Confluency1Versene (ml of 0.05% EDTA)Trypsin (ml of 0.05% tryspin, 0.53 mM EDTA)Growth Medium (ml)
Dishes      
35mm90.3 x 1061.2 x 10611  2
60mm210.8 x 1063.2 x 106323
100mm552.2 x 1068.8 x 1065310
150mm1525.0 x 10620.0 x 10610820
Culture Plates      
6-well90.3 x 1061.2 x 10622  3 - 5
12-well40.1 x 1060.4 x 106111- 2
24-well20.05 x 1060.2 x 1060.50.50.5 - 1.0
Flasks      
T-25250.7 x 1062.8 x 106333 - 5
T-75752.1 x 1068.4 x 106558 - 15
T-1601624.6 x 10618.4 x 106101015 - 30


1The number of cells on a confluent plate, dish, or flask will vary with cell type.  For this table, HeLa cells were used.