Mammalian Cell Lines

Cell Line
Cell Type
Species
Tissue
Medium*
293
fibroblast
human
embryonic kidney
MEM and 10% FBS
3T6
fibroblast mouse
embryo
DMEM and 10% FBS
A549
epithelial
human
lung carcinoma
F-12K and 10% FBS
A9
fibroblast mouse
connective tissue
DMEM and 10% FBS
AtT-20
epithelial
mouse
pituitary tumor
F-10, 15% horse serum and 2.5% FBS
BALB/3T3
fibroblast
mouse
embryo
DMEM and 10% FBS
BHK-21
fibroblast
hamster
kidney
GMEM and 10% FBS or MEM and 10% FBS plus NEAA
BHL-100
epithelial
human
breast
McCoy's 5A and 10% FBS
BT
fibroblast
bovine
turbinate cells
MEM and 10% FBS plus NEAA
Caco-2
epithelial
human
colon adeno carcinoma
MEM and 20% FBS plus NEAA
Chang
epithelial
human
liver
BME and 10% calf serum
CHO-K1
epithelial
hamster
ovary
F-12 and 10% FBS
Clone 9
epithelial
rat
liver
F-12K and 10% FBS
Clone M-3
epithelial
mouse
melanoma
F-10, 15% horse serum and 2.5% FBS
COS-1, COS-3, COS-7
fibroblast
monkey
kidney
DMEM and 10% FBS
CRFK
epithelial
cat
kidney
MEM and 10% FBS plus NEAA
CV-1
fibroblast
monkey
kidney
MEM and 10% FBS
D-17
epithelial
dog
osteosarcoma
MEM and 10% FBS and NEAA
Daudi
lymphoblast
human
blood from a lymphoma patient
RPMI-1640 and 10% FBS
GH1, GH3
epithelial
rat
pituitary tumor
F-10, 15% horse serum and 2.5% FBS
H9
lymphoblast
human
T-cell lymphoma
RPMI-1640 and 20% FBS
HaK
epithelial
hamster
kidney
BME and 10% calf serum
HCT-15
epithelial
human
colorectal adenocarcinoma
RPMI-1640 and 10% FBS
HeLa
epithelial
human
cervix carcinoma
MEM and 10% FBS plus NEAA (in suspension, S-MEM)
HEp-2
epithelial
human
larynx carcinoma
MEM and 10% FBS
HL-60
lymphoblast
human
promyeolocytic leukemia
RPMI-1640 and 20% FBS
HT-1080
epithelial
human
fibrosarcoma
MEM and 10% HI FBS plus NEAA
HT-29
epithelial
human
colon adenocarcinoma
McCoy's 5A and 10% FBS
HUVEC
endothelial
human
umbilical cord
F-12K and 10% FBS plus 100 µg/ml heparin
I-10
epithelial
mouse
testicular tumor
F-10, 15% horse serum and 2.5% FBS
IM-9
lymphoblast
human
marrow from myeloma patient
RPMI-1640 and 10% FBS
JEG-2
epithelial
human
choriocarcinoma
MEM and 10% FBS
Jensen
fibroblast
rat
sarcoma
McCoy's 5A and 5% FBS
Jurkat
lymphoblast
human
lymphoma
RPMI-1640 and 10% FBS
K-562
lymphoblast
human
myelogenous leukemia
RPMI-1640 and 10% FBS
KB
epithelial
human
oral carcinoma
MEM and 10% FBS plus NEAA
KG-1
myeloblast
human
marrow from erythroleukemia patient
IMDM and 20% FBS
L2
epithelial
rat
lung
F-12K and 10% FBS
LLC-WRC 256
epithelial
rat
carcinoma
Medium 199 and 5% horse serum
McCoy
fibroblast
mouse
unknown
MEM and 10% FBS
MCF7
epithelial
human
breast adenocarcinoma
MEM and 10% FBS plus NEAA and 10 µg/ml insulin
WI-38
epithelial
human
embryonic lung
BME and 10% FBS
WISH
epithelial
human
amnion
BME and 10% FBS
XC
epithelial
rat
sarcoma
MEM and 10% FBS plus NEAA
Y-1
epithelial
mouse
tumor of adrenal
F-10, 15% horse serum and 2.5% FBS

*BME: Basal Medium Eagle; DMEM: Dulbecco’s Modified Eagle Medium; FBS: Fetal Bovine Serum; GMEM: Glasgow Minimum Essential Medium; IMDM: Iscove’s Modified Dulbecco’s Medium; MEM: Minimum Essential Medium; NEAA: Non-Essential Amino Acids Solution; TNM-FH: Trichoplusia ni Medium-Formulation Hink (i.e., Grace’s Insect Medium, Supplemented).


Insect Cell Lines


Cell Line
Species
Tissue
*Media
Sf9, Sf21
fall army worm
(Spodoptera frugiperda)
pupal ovary
TNM-FH and 10% FBS, or Sf-900 II SFM (serum-free), or Sf-900™ III SFM (serum-free)
High Five™ (BTI-TN-5B1-4)
cabbage looper
(Trichoplusia ni)
ovary
TNM-FH and 10% FBS, or Express Five® SFM (serum-free)
Schneider 2 (S2), D.Mel-2
fruit fly
(Drosophila melanogaster)

Schneider’s Drosophila medium and 10% heat-inactivated FBS

* BME: Basal Medium Eagle; DMEM: Dulbecco’s Modified Eagle Medium; FBS: Fetal Bovine Serum; GMEM: Glasgow Minimum Essential Medium; IMDM: Iscove’s Modified Dulbecco’s Medium; MEM: Minimum Essential Medium; NEAA: Non-Essential Amino Acids Solution; TNM-FH: Trichoplusia ni Medium-Formulation Hink (i.e., Grace’s Insect Medium, Supplemented).