Researchers have a wide variety of gene delivery techniques to introduce plasmid DNA, siRNA or duplex RNAi, oligonucleotides, and RNA into eukaryotic cells for a variety of research and drug discovery applications.  For more information on the most appropriate transfection method for your cell line and application, refer to our Transfection Selection Guide.

Plasmid Transfection

Invitrogen offers the most complete collection of cationic lipid-based transfection reagents with exceptional performance that can be used for plasmid delivery into a broad range of cells.  Lipofectamine™ LTX with PLUS™ Reagent (Cat. no. 15338-100) is a plasmid DNA-specific transfection reagent that provides maximum expression with minimum cytotoxicity.

Viral Delivery Systems

Having evolved to proficiently deliver nucleic acids to cells, viruses offer a means to reach hard-to-transfect cell types for protein overexpression or knockdown.  Adenoviral, oncoretroviral, and lentiviral vectors have been used extensively for delivery in cell culture and in vivo.  Invitrogen offers a variety of products used in viral-mediated transduction, including BLOCK-iT™ Adenoviral and Lentiviral Expression Systems.

RNAi Transfection

RNA interference (RNAi) technology is revolutionizing the biological discovery process as well as target discovery and validation.  Using RNAi, you can turn gene expression “off”, or knock it down, to better understand its function and role in disease.  High efficiency transfection is an essential first step for achieving effective gene knockdown.  Invitrogen offers the most complete collection of cationic lipid-based transfection reagents with exceptional performance that can be used for delivery of assorted RNAi reagents, including shRNA and miRNA RNAi vectors and synthetic molecules such as siRNA, Stealth RNAi™ siRNA, and Dicer-generated siRNAi pools.  Further, cell specific RNAi transfection protocols have been developed using these transfection reagents for many popular cell lines.  

  • For more information and a complete list, refer to our resources on RNAi Transfection

Transient Protein Production & High Throughput Screening

For the highest protein yields in mammalian suspension cells, FreeStyle™ MAX Transfection Reagent (Cat. no. 16447-100) is a versatile and scalable transient expression tool for CHO and 293 suspension cell cultures.  FreeStyle™ MAX Reagent is a proprietary, animal origin-free formulation for the transfection of plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins.  FreeStyle™ MAX Reagent allows the highest expression levels and transfection rates with lowest cytotoxicity in bio-production applications.

Neon™ Transfection System

 The Neon™ Transfection System (Cat. no. MPK5000) is a novel, benchtop electroporation device that employs an electroporation technology by using the pipette tip as an electroporation chamber to efficiently transfect mammalian cells including primary and immortalized hematopoietic cells, stem cells, and primary cells.  The Neon™ Transfection System efficiently delivers nucleic acids, proteins, and siRNA into all mammalian cell types including primary and stem cells with a high cell survival rate.