We provide a vast number of stand-alone reagents for preparing bioconjugates, most of which are described in detail in other sections of this chapter. This section describes the many specialized kits that we have developed for covalently labeling proteins and nucleic acids with our organic dyes and haptens (Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3).

As an alternative to direct conjugation of primary antibodies with our reactive dyes and haptens, we recommend using Zenon technology (Zenon Technology: Versatile Reagents for Immunolabeling—Section 7.3) to form labeled antibody complexes. Zenon labeling can be completed in minutes with quantitative yield starting with submicrogram quantities of antibody, and the labeling stoichiometry can be easily adjusted to deliver optimum fluorescence output. Although they do not provide covalent labeling, the Zenon Antibody Labeling Kits are listed in Active esters and kits for labeling proteins and nucleic acids—Table 1.2, as well as in Zenon Antibody Labeling Kits—Table 7.7.

Kits for Labeling Proteins with a Fluorescent Dye

APEX Antibody Labeling Kits

APEX Antibody Labeling Kits (Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) provide a convenient method for covalently labeling small amounts (10–20 µg) of IgG antibody with Alexa Fluor dyes, Oregon Green 488 dye or Pacific Blue dye (or with biotin, see below). A primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding than secondary antibodies. Furthermore, multiple primary antibodies of the same isotype or derived from the same species can be used in the same immunostaining experiment if they are directly labeled with compatible fluorophores. Many IgG antibodies, however, are often only available in small quantities and may be mixed with stabilizing proteins, such as BSA, that can react nonproductively with the amine-reactive labeling reagents.

APEX Antibody Labeling Kits are specifically designed to allow labeling of small amounts of IgG antibody, even in the presence of contaminants. These kits utilize a solid-phase labeling technique that captures the IgG antibody on resin inside an APEX antibody labeling tip (Figure 1.2.1). Any contaminants, including stabilizing proteins or amine-containing buffers, are eluted through the tip before labeling. After applying the amine-reactive fluorophore to the IgG antibody trapped on the resin, a fluorescent IgG conjugate is formed and subsequently eluted from the resin using elution buffer. The fluorescent IgG conjugate is ready to use in an imaging or flow cytometry assay in as little as 2.5 hours with minimal hands-on time. The typical yield of labeled antibody using this method is between 40 and 80%.

Each APEX Antibody Labeling Kit provides all reagents required to perform five separate labeling reactions of 10–20 µg IgG antibody, including:

  • Five vials of amine-reactive fluorescent dye
  • Five APEX antibody labeling tips
  • Wash, labeling, neutralization and elution buffers
  • Dimethylsulfoxide (DMSO)
  • Labeling protocol (APEX Antibody Labeling Kits)

For labeling larger amounts of protein, we recommend the Alexa Fluor Microscale Protein Labeling Kits, which are optimized for 20–100 µg samples of proteins between 10,000 and 150,000 daltons; the Alexa Fluor Antibody Labeling Kits, which are optimized for 100 µg samples of mono- or polyclonal antibodies; or the Alexa Fluor Protein Labeling Kits, which are optimized for 1 mg samples of >40,000-dalton proteins.

APEX antibody labeling  

Figure 1.2.1 Illustration of the use of the APEX antibody labeling tip, provided in APEX Antibody Labeling Kits. A) Applying solutions to the resin in the tip. B) Pushing solutions onto the resin in the tip by attaching the APEX antibody labeling tip to a pipette.

SiteClick Antibody Labeling Kits

The SiteClick system is a modular, click chemistry–mediated method for enzymatically labeling essentially any antibody on its heavy chain N-linked glycans (Figure 1.2.2). This antibody labeling method allows simple and gentle site-selective attachment of detection molecules to heavy chain N-linked glycans—far from the antigen-binding domain—providing excellent reproducibility from labeling to labeling and from antibody to antibody. The SiteClick antibody labeling method prevents disruption of the antigen-binding domain that can occur with traditional amine- or thiol-reactive labeling reagents and eliminates the need to genetically engineer labeling sites into the antibody prior to modification. This site-selective strategy is especially important when labeling monoclonal antibodies that contain lysine residues in or around the antigen-binding domain, as labeling of these sites can disrupt antigen binding. Monoclonal antibodies can also have variable sensitivities to disulfide bond–reducing agents used in reductive cysteine labeling, leaving partially dissociated antibody fragments that result in decreased antibody binding and yield. A number of different detection molecules can be site-selectively attached to the heavy chain glycans—including phycobiliproteins (e.g., R-PE), Qdot probes, fluorescent dyes, metal-chelating compounds, and other small molecules like biotin—allowing multiplex analysis with antibodies from the same species (Figure 1.2.3).

In general, IgG antibodies contain two N-linked glycans attached to specific conserved asparagine residues located in the antibody heavy chain Fc domain. These sugar chains, predominantly complex biantennary glycans with two terminal branches, are structurally quite homogeneous, and the terminal sequences of the glycan branches are highly consistent. Most of the antibody glycan branches terminate with galactose-N-acetylglucosamine (Gal-GlcNAc-) or with N-acetylglucosamine (GlcNAc-). Removal of the terminal Gal residue with β-galactosidase unmasks the majority of terminal GlcNAc labeling sites for the subsequent enzymatic β-galactosyl transferase (GalT) reaction (Figure 1.2.2). Although a small percentage of antibodies also contain terminal sialic acids, our experiments have shown that the additional removal of terminal sialic acid residues produces little difference in the final degree of antibody labeling. After cleavage of terminal Gal residues by β-galactosidase, each N-linked glycan will contain, on average, 2 terminal GlcNAc residues per heavy chain (4 terminal GlcNAc per antibody). In practice, the degree of labeling of mouse and rabbit monoclonal antibodies, as determined by subsequent attachment of fluorescent DIBO dyes, averages between 3 and 4 labels per antibody.

The SiteClick enzymatic labeling approach takes advantage of the modified β-GalT1 enzyme GalT(Y289L), which is substrate permissive and attaches azide-modified N-acetylgalactosamine (GalNAz) selectively to terminal GlcNAc residues on N-linked antibody glycans ref (Figure 1.2.4). These azide-activated antibodies are then reacted with dibenzocyclooctyne (DIBO)-functionalized molecules in a copper-free click reaction, which produces a strong and stable bond between the DIBO detection molecule and the azide-modified antibody.ref By coupling GalT(Y289L) enzymatic labeling with a click reaction, the SiteClick system achieves highly efficient and reproducible site-selective labeling of antibodies, regardless of their originating species or antibody class.

Our SiteClick products include R-PE antibody labeling kits, which are designed especially for flow cytometry applications, as well as several Qdot antibody labeling kits that produce conjugates for imaging or flow cytometry applications. The kits are configured to provide an easy-to-follow workflow that will allow novice and experienced scientists alike to obtain efficient antibody labeling every time. And, because antibody glycans are highly conserved, even between different species, the reproducibility of SiteClick labeling for different antibodies is very high, precluding the need to optimize labeling of each newly acquired antibody. Each SiteClick Antibody Labeling Kit provides all reagents required to perform one conjugation reaction of R-PE or Qdot fluorophores to 100–125 µg IgG of purified IgG antibody, including:

  • DIBO-modified R-PE or Qdot fluorescent label
  • Labeling buffers and additive
  • β-Galactosidase
  • UDP-GalNAz
  • β-1,4-galactosyltransferase (GalT)
  • Antibody concentrators and collection tube
  • Labeling protocol (SiteClick Antibody Labeling Kits)

The SiteClick antibody labeling kits are compatible with antibodies from a number of different species including, but not limited to, human, rabbit, mouse, rat, goat, hamster, and chicken. Additionally, SiteClick labeling is effective with several antibody classes such as IgG, IgM, and IgY; note that chicken IgY antibodies have 6 heavy chain glycans instead of 2 and therefore can be labeled to a higher extent.

SiteClick antibody labeling systemFigure 1.2.2 The SiteClick antibody labeling system. The first step in the SiteClick antibody labeling process involves removal of terminal galactose residues from the heavy chain N-linked glycans using β-galactosidase, exposing essentially all possible modifiable GlcNAc residues. Second, the free terminal GlcNAc residues are activated with azide tags by enzymatic attachment of GalNAz to the terminal GlcNAc residues using the GalT(Y289L) enzyme. In the third step, the azide residues are reacted with the dibenzocyclooctyne (DIBO)-functionalized probe of choice (e.g., Alexa Fluor 488 DIBO alkyne (C10405)). The average degree of labeling is 3–3.5 labels per antibody.

Immunocytochemistry with SiteClick™ labeled antibodies  
Figure 1.2.3 Immunocytochemistry with SiteClick labeled antibodies. HeLa cells were fixed, permeabilized, and incubated with 10 nM Qdot 655 anti–golgin 97 antibody (magenta). This conjugate was generated using the SiteClick Qdot® 655 Antibody Labeling Kit (S10453) and mouse monoclonal anti–golgin-97 antibody (A21270). After antibody incubation, the cells were counterstained with NucBlue Live (R37605) and ActinGreen 488 (R37110) ReadyProbes reagents prior to imaging.
Site selectivity of SiteClick™ antibody labeling  
Figure 1.2.4 Site selectivity of SiteClick antibody labeling. A) A β-tubulin monoclonal antibody was azide-activated using the GalT(Y289L) enzyme, labeled with DIBO-functionalized Alexa Fluor 488 dye, and then analyzed by gel electrophoresis (left panel). Once imaged, the same gel was post-stained with SYPRO Ruby Total Protein Gel Stain (S12000, right panel), showing that only the heavy chain of the antibody is click-labeled with the Alexa Fluor 488 DIBO alkyne (C10405). B) Anti–β-tubulin mouse monoclonal antibodies were labeled with a small-molecule DIBO-PET chelating agent (L) or left unlabeled (U) (left two lanes) and then post-treated with PNGase F, which selectively cleaves N-linked glycans from asparagine residues (right two lanes). After treatment with PNGase F, both chelator-labeled (L) and unlabeled (U) species are shifted to the same lower molecular weight, confirming the site-selective labeling of heavy chain N-linked glycans.

Alexa Fluor, Pacific Blue and Pacific Orange Antibody Labeling Kits

The Alexa Fluor, Pacific Blue and Pacific Orange Antibody Labeling Kits (Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) provide researchers with a simple yet efficient means of labeling small amounts of  antibodies with Alexa Fluor dyes or with Pacific Blue or Pacific Orange dye (Figure 1.2.5). These kits contain everything needed to perform five separate labeling reactions containing ~100 µg each of carrier-free antibody samples (although other proteins can be labeled).

Each Antibody Labeling Kit provides:

  • Five vials of amine-reactive fluorescent dye (succinimidyl ester or tetrafluorophenyl ester)
  • Sodium bicarbonate
  • Spin columns and collection tubes
  • Detailed protocols for conjugation, purification and determination of the degree of labeling (Antibody Labeling Kits)

Simply dissolve the carrier-free antibody at ~1 mg/mL in the provided buffer, then add it to one of the five vials of amine-reactive dye; no organic solvents are required. Purification of the fluorescent conjugate is accomplished on a size-exclusion spin column optimized for proteins with molecular weight greater than 40,000 daltons. Labeling and purification can be completed in less than 2 hours.

Mouse antibodies in serum, in ascites fluid or diluted with carrier proteins should not be labeled with these kits; however, such antibody preparations can be efficiently labeled with the APEX Antibody Labeling Kits described above or with the Zenon Mouse IgG Labeling Kits (Zenon Technology: Versatile Reagents for Immunolabeling—Section 7.3, Zenon Antibody Labeling Kits—Table 7.7) described below.

Monoclonal Antibody Labeling Kits
Figure 1.2.5
Illustration of the three simple steps in the protocol for the Alexa Fluor, Pacific Blue and Pacific Orange Antibody Labeling Kits, which provide a convenient method for covalently labeling small amounts of  antibodies.

SAIVI Antibody Labeling Kits

The optimal fluorescent antibody conjugate for in vitro detection assays produces an intense fluorescent signal yet retains the binding affinity and specificity of the unlabeled antibody. When preparing a fluorescent antibody conjugate for in vivo animal imaging, however, the pharmacokinetics of the labeled probe must also be considered.ref These additional constraints have led to the development of the SAIVI Antibody Labeling Kits for small animal in vivo imaging applications (Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3).

SAIVI Antibody Labeling Kits feature reactive far-red and near-infrared Alexa Fluor dyes, along with a labeling protocol specifically designed to produce a suitable degree of labeling (DOL) for in vivo imaging applications. When optimally labeled, a fluorescent antibody conjugate produces an intense, targeted fluorescent signal that persists throughout the in vivo study, without significant redistribution or clearance of the probe.

The conjugation protocol in the SAIVI Rapid Antibody Labeling Kits produces an optimal DOL (~2 fluorophores per antibody) over a sixfold antibody concentration range with no adjustments in reaction volume, dye concentration or antibody concentration necessary. Purification of the dye-labeled antibody is achieved using a simple protocol that can be completed in less than 10 minutes. With these kits, optimally labeled antibodies are ready for applications that require azide-free reagents, such as live-cell imaging or direct injections into animals. SAIVI Rapid Antibody Labeling Kits containing either Alexa Fluor 680 or Alexa Fluor 750 dye (S30045, S30046) provide sufficient reagents for three labeling reactions of 0.5–3 mg of protein each, including:

  • Three vials of amine-reactive Alexa Fluor 680 or Alexa Fluor 750 dye
  • Sodium bicarbonate
  • Regulator solution
  • Purification resin and purification columns
  • Phosphate-buffered saline (PBS)
  • Syringes, syringe filters, column-loading pipettes and catch tubes
  • Detailed protocols for conjugation, purification and determination of the degree of labeling (SAIVI Rapid Antibody Labeling Kits)

To control the DOL of the antibody conjugate, the SAIVI Alexa Fluor 647 Antibody/Protein Labeling Kit (S30044) includes a DOL modulating reagent and instructions for decreasing the DOL from its intrinsic highest value by adding specific amounts of this reagent to the labeling reaction. With this method, antibody preparations with varying ratios of dye to protein can be quickly and reproducibly obtained without significant alteration of labeling or purification conditions, allowing more efficient optimization for in vivo imaging applications. Each kit provides sufficient reagents for three labeling reactions of 1 mg protein each, including:

  • Three vials of amine-reactive Alexa Fluor 647 dye
  • Sodium bicarbonate
  • DOL modulating reagent
  • Purification resin and purification columns
  • Wash buffer
  • Column funnels, column holders, disposable pipettes and collection tubes
  • Detailed protocols for conjugation, purification and determination of the degree of labeling (SAIVI Alexa Fluor 647 Antibody/Protein Labeling Kit)

Alexa Fluor Microscale Protein Labeling Kits

Alexa Fluor Microscale Protein Labeling Kits (Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) provide a convenient means for labeling small amounts (20–100 µg) of purified protein with the highly fluorescent Alexa Fluor dyes. Spin columns are used to purify the labeled protein, with yields between 60 and 90% depending primarily on the molecular weight of the starting material. Labeling and purification can be completed in as little as 30 minutes.

These kits have been optimized for labeling proteins with molecular weights between 12,000 and 150,000 daltons and contain everything needed to perform three labeling reactions and to separate the resulting conjugates from excess dye. Each Alexa Fluor Microscale Protein Labeling Kit provides:

  • Three vials of amine-reactive fluorescent dye (succinimidyl ester or tetrafluorophenyl ester)
  • Sodium bicarbonate
  • Reaction tubes
  • Purification resin and spin filters
  • Detailed protocols for conjugation, purification and determination of the degree of labeling (Alexa Fluor Microscale Protein Labeling Kits

Easy-to-Use Protein Labeling Kits

Our Protein Labeling Kits (Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) provide a nearly effortless way to label proteins, especially IgG antibodies, with a fluorescent dye (Figure 1.2.6). Simply add ~1 mg of protein (in a volume of ~500 µL and free of amine-containing buffers such as Tris) to one of the three included vials, which contain a premeasured quantity of amine-reactive dye and a magnetic stir bar. Because the reactive dyes used in these kits are water soluble, no organic solvents are required. Purification is accomplished on a gravity-feed size-exclusion column, which is supplied with the kit. Labeling and purification can be completed in about 2 hours, with very little hands-on time.

Each Protein Labeling Kit provides sufficient reagents for labeling three ~1 mg protein samples of a 150,000-dalton protein (such as an IgG), including:

  • Three vials of the amine-reactive fluorescent dye (succinimidyl ester or tetrafluorophenyl ester), each containing a magnetic stir bar
  • Sodium bicarbonate
  • Gravity-feed columns, a size-exclusion resin and concentrated elution buffer
  • Column funnels, foam column holders, disposable pipettes and collection tubes
  • Detailed protocols for conjugation, purification and determination of the degree of labeling (Protein Labeling Kits)

Researchers have modified α-synuclein using either the Oregon Green 488 Protein Labeling Kit (O10241) or the Alexa Fluor 594 Protein Labeling Kit (A10239).ref They intentionally produced fluorescent conjugates with a low degree of labeling (DOL) so as not to perturb oligomerization. When comparing Oregon Green 488 synuclein with Alexa Fluor 594 synuclein, they observed essentially the same results in terms of fibril formation as analyzed by fluorescence polarization. They also detected the Oregon Green 488 synuclein conjugate with mouse monoclonal anti–fluorescein/Oregon Green dye antibody (monoclonal 4-4-20, A6421; Anti-Dye and Anti-Hapten Antibodies—Section 7.4), and gold-labeled anti–mouse IgG antibody using electron microscopy.

Protein Labeling Kits
Figure 1.2.6
Illustration of the three simple steps in the protocol for the Easy-to-Use Protein Labeling Kits, which provide a convenient method for covalently labeling most proteins.

FluoReporter Protein Labeling Kits

The FluoReporter Protein Labeling Kits (Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) facilitate research-scale preparation of protein conjugates labeled with some of our brightest fluorescent dyes. Typically, labeling and purifying conjugates with the FluoReporter Protein Labeling Kits can be completed in under 3 hours, with very little hands-on time. First, the amount of dye necessary for the desired protein sample is calculated using the guidelines outlined in the kit protocol. After dissolving the dye in dimethylsulfoxide (DMSO), the calculated amount of dye is added to the protein and the reaction is incubated for 1–1.5 hours. Purification is easily accomplished using convenient spin columns designed for use with proteins of molecular weight greater than 30,000 daltons.

Each FluoReporter Protein Labeling Kits provides sufficient reagents to label 5 to 10 protein samples of 0.2–2 mg each in 200 µL volumes :

  • Five vials of the amine-reactive dye
  • Dimethylsulfoxide (DMSO)
  • Reaction tubes, each containing a stir bar
  • Spin columns and collection tubes
  • Detailed protocols for conjugation, purification and determination of the degree of labeling

Zenon Antibody Labeling Kits

The Zenon Antibody Labeling Kits (Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Zenon Antibody Labeling Kits—Table 7.7) are useful for the rapid and quantitative labeling of antibodies with dyes (including phycobiliproteins and their tandem conjugates), biotin or enzymes ref (Figure 1.2.7). Zenon Antibody Labeling Kits are designed to label intact antibodies in amounts from less than 1 µg to as much as 50 µg, starting with a purified antibody fraction or with a crude antibody preparation such as serum, ascites fluid or a hybridoma supernatant. The Zenon antibody labeling technology is discussed further in Zenon Technology: Versatile Reagents for Immunolabeling—Section 7.3.

Zenon Antibody Labeling Kits
Figure 1.2.7
Labeling scheme utilized in the Zenon Antibody Labeling Kits. A) An unlabeled IgG antibody is incubated with the Zenon labeling reagent, which contains a fluorophore-labeled, Fc-specific anti-IgG Fab fragment. B) This labeled Fab fragment binds to the Fc portion of the IgG antibody. C) Excess Fab fragment is then neutralized by the addition of a nonspecific IgG, preventing crosslabeling by the Fab fragment in experiments where primary antibodies of the same type are present. Note that the Fab fragment used for labeling need not be coupled to a fluorophore, but could instead be coupled to an enzyme (such as HRP) or to biotin.

Kits for Labeling Proteins with Biotin or Dinitrophenyl (DNP)

APEX Biotin-XX Antibody Labeling Kit

The APEX Biotin-XX Antibody Labeling Kit (A10495, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) provides a convenient method for covalently labeling small amounts (10–20 µg) of IgG antibody with biotin-XX sulfosuccinimidyl ester (biotin-XX SSE). The water-soluble biotin-XX SSE has a 14-atom spacer (structure) that enhances the binding of biotin derivatives to avidin's relatively deep binding sites. APEX Antibody Labeling Kits are specifically designed to allow labeling of small amounts of IgG antibody, even in the presence of contaminants. These kits utilize a solid-phase labeling technique that captures the IgG antibody on resin inside an APEX antibody labeling tip (Figure 1.2.1). Any contaminants, including stabilizing proteins or amine-containing buffers, are eluted through the tip before labeling. After applying the amine-reactive biotin to the IgG antibody trapped on the resin, a biotinylated IgG conjugate is formed and subsequently eluted from the resin using elution buffer. The biotinylated IgG conjugate is ready to use in an imaging or flow cytometry assay in as little as 2.5 hours with minimal hands-on time. The typical yield of labeled antibody using this method is between 40 and 80%.

Each APEX Biotin-XX Antibody Labeling Kit provides all reagents required to perform five separate labeling reactions of 10–20 µg IgG antibody, including:

  • Five vials of amine-reactive biotin-XX sulfosuccinimidyl ester (biotin-XX SSE)
  • Five APEX antibody labeling tips
  • Wash, labeling, neutralization and elution buffers
  • Dimethylsulfoxide (DMSO)
  • Labeling protocol (APEX Antibody Labeling Kits

For labeling larger amounts of protein, we recommend the Biotin-XX Microscale Protein Labeling Kit, which is optimized for 20–100 µg samples of proteins between 10,000 and 150,000 daltons; the FluoReporter Mini-Biotin-XX Protein Labeling Kit, which is optimized for 0.1–3 mg samples of >30,000-dalton proteins; or the FluoReporter Biotin-XX Protein Labeling Kit, which is optimized for 5–20 mg samples.

Biotin-XX Microscale Protein Labeling Kits

The Biotin-XX Microscale Protein Labeling Kit (B30010; Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) provides a convenient means for biotinylating small amounts (20–100 µg) of purified protein with biotin-XX sulfosuccinimidyl ester (biotin-XX SSE). Spin columns are used to purify the labeled protein, with yields between 60 and 90% depending primarily on the molecular weight of the starting material. Labeling and purification can be completed in as little as 30 minutes.

This kit, which has been optimized for labeling proteins with molecular weights between 12,000 and 150,000 daltons, contains everything needed to perform three labeling reactions and to separate the resulting conjugates from excess reactive biotin. Each Biotin-XX Microscale Protein Labeling Kit provides:

  • Three vials of amine-reactive biotin-XX sulfosuccinimidyl ester (biotin-XX SSE)
  • Sodium bicarbonate
  • Reaction tubes
  • Purification resin and spin filters
  • Detailed protocols for conjugation and purification (Biotin-XX Microscale Protein Labeling Kit)

For determining the degree of labeling, the FluoReporter Biotin Quantitation Assay Kit for proteins is available separately (F30751) or in combination with the Biotin-XX Microscale Protein Labeling Kit (B30756). When biotinylating larger amounts of protein, we recommend the FluoReporter Mini-Biotin-XX Protein Labeling Kit, which is optimized for 0.1–3 mg samples of >30,000-dalton proteins, or the FluoReporter Biotin-XX Protein Labeling Kit, which is optimized for 5–20 mg samples.

FluoReporter Mini-Biotin-XX Protein Labeling Kit

The FluoReporter Mini-Biotin-XX Protein Labeling Kit (F6347; Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) permits efficient biotinylation of small amounts of antibodies or other proteins with biotin-XX sulfosuccinimidyl ester (biotin-XX SSE). The ready-to-use spin columns provide a convenient method of purifying the biotinylated protein from excess reagents.

Each FluoReporter Mini-Biotin-XX Protein Labeling Kit provides sufficient reagents for five labeling reactions of 0.1–3 mg protein each, including:

  • Five vials of amine-reactive biotin-XX sulfosuccinimidyl ester (biotin-XX SSE)
  • Reaction tubes, each containing a stir bar
  • Purification resin, spin columns and collection tubes
  • Dialysis tubing
  • Detailed protocols for conjugation and purification (FluoReporter Mini-Biotin-XX Protein Labeling Kit

FluoReporter Biotin-XX Protein Labeling Kit

The FluoReporter Biotin-XX Protein Labeling Kit (F2610; Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) is designed to provide five biotinylation reactions, each containing 5–20 mg of protein. A gel filtration column is used to purify the labeled proteins from excess biotin reagent. Once purified, the degree of biotinylation can be determined using the included avidin–HABA displacement assay; biotinylated goat IgG is provided as a standard.

Each FluoReporter Biotin-XX Protein Labeling Kit provides sufficient reagents for five labeling reactions of 5–20 mg protein each, including:

  • Amine-reactive biotin-XX succinimidyl ester
  • Dimethylsulfoxide (DMSO)
  • Gel filtration column
  • Avidin–HABA complex
  • Biotinylated goat IgG
  • Protocols for preparing and purifying the biotinylated protein, as well as for quantitating the degree of labeling

FluoReporter Biotin/DNP Protein Labeling Kit

The FluoReporter Biotin/DNP Protein Labeling Kit (F6348; Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) provides the necessary reagents for labeling proteins with DNP-X–biocytin-X succinimidyl ester (structure). The degree of biotinylation of proteins labeled with this reagent can be assessed from the optical absorbance of DNP (EC = 15,000 cm-1M-1 at ~360 nm). The conjugates are recognized by both avidin derivatives (and anti-biotin antibodies) and by anti-DNP antibodies, permitting a choice of detection techniques.

Each FluoReporter Biotin/DNP Protein Labeling Kit contains sufficient reagents for 5 to 10 labeling reactions of 0.2–2 mg of protein each, including:

  • Five vials of amine-reactive DNP-X–biocytin-X succinimidyl ester
  • Dimethylsulfoxide (DMSO) for dissolving the succinimidyl ester
  • Reaction tubes
  • Spin columns and collection tubes
  • Detailed protocols for conjugation, purification and determination of the degree of labeling

DSB-X Biotin Protein Labeling Kit

Our unique DSB-X biotin technology, which is described in detail in Avidin, Streptavidin, NeutrAvidin and CaptAvidin Biotin-Binding Proteins and Affinity Matrices—Section 7.6, permits the facile reversal of the biotin–avidin interaction under extremely gentle conditions.ref DSB-X biotin succinimidyl ester, a derivative of desthiobiotin (Figure 1.2.8) with an additional seven-atom spacer, reacts with amine groups of biomolecules to form stable amides. Like biotin conjugates, the DSB-X biotin conjugate can be detected with any avidin or streptavidin derivative; with DSB-X biotin conjugates, however, this binding is almost totally reversed by addition of free biotin (B1595, B20656; Biotinylation and Haptenylation Reagents—Section 4.2) at neutral pH and normal ionic strength.

The DSB-X Biotin Protein Labeling Kit (D20655; Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) contains the reagents required for five protein conjugations of 0.5–3 mg each, including:

  • Five vials of amine-reactive DSB-X biotin succinimidyl ester
  • Dimethylsulfoxide (DMSO) for dissolving the succinimidyl ester
  • Reaction tubes
  • Purification resin, spin columns and collection tubes for small-scale purifications
  • Dialysis tubing for larger-scale separations
  • Detailed protocols for conjugation and purification (DSB-X Biotin Protein Labeling Kit)
Structures of D-biotin & D-desthiobiotin  
Figure 1.2.8 Comparison of the structures of D-biotin (top) and D-desthiobiotin (bottom).

Kits for Labeling Nucleic Acids with a Fluorescent Dye

ULYSIS Nucleic Acid Labeling Kits

The ULYSIS Alexa Fluor Nucleic Acid Labeling Kits (Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3, Molecular Probes nucleic acid labeling kits—Table 8.6) provide a simple, reliable method for producing fluorescent hybridization probes by combining our Alexa Fluor fluorophores with the versatile, patented Universal Linkage System (ULS) platinum-based chemistry developed by KREATECH Biotechnology BV. The ULS technology is based on the use of a platinum dye reagent that forms a stable adduct with the N-7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, peptide–nucleic acid conjugates (PNA) and oligonucleotides (Figure 1.2.9). In protein contexts, ULS reagents are reactive with cysteine residues and other thiols.ref The labeling reaction takes only 15 minutes, and separation of the labeled nucleic acids from the unreacted ULS reagent can be accomplished through use of a simple spin-column procedure (Figure 1.2.10).

In addition to ULYSIS Alexa Fluor Nucleic Acid Labeling Kits, we offer the ULYSIS Oregon Green 488 Nucleic Acid Labeling Kit (Labeling Oligonucleotides and Nucleic Acids—Section 8.2).  Each of these ULYSIS Kits provides sufficient reagents for 20 labelings of 1 µg DNA each, including:

  • ULS labeling reagent and appropriate solvent
  • Labeling buffer
  • Deoxyribonuclease I (DNase I), for digesting DNA longer than 1000 base-pairs prior to labeling
  • DNase I storage and reaction buffers
  • Control DNA from calf thymus
  • Nuclease-free H2O
  • Detailed protocols for preparing fluorescent DNA hybridization probes for chromosome in situ hybridization and dot-blot hybridization (ULYSIS Nucleic Acid Labeling Kits)

Probes labeled using the ULYSIS Kits are stable indefinitely and hybridize effectively to target DNA. The ULS method has been used to prepare labeled probes for dot, Southern and northern blot analysis, RNA and DNA in situ hybridization, multicolor FISH, comparative genome hybridization (CGH) and microarray analysis.

ULYSIS Nucleic Acid Labeling Kits  

Figure 1.2.9 Schematic diagram of the labeling method provided in our ULYSIS Nucleic Acid Labeling Kits. The ULS reagent in the ULYSIS Nucleic Acid Labeling Kits reacts with the N-7 position of guanine residues to provide a stable coordination complex between the nucleic acid and the fluorophore label.

Nucleic acid labeling
Figure 1.2.10 Nucleic acid labeling method provided in our ULYSIS Nucleic Acid Labeling Kits.

ARES DNA Labeling Kits

The ARES DNA Labeling Kits (Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3, Molecular Probes nucleic acid labeling kits—Table 8.6) provide a versatile, two-step method for labeling DNA with several of our premier fluorescent dyes ref (Figure 1.2.11). In the first step, an amine-modified nucleotide, 5-(3-aminoallyl)-dUTP (structure), is incorporated into DNA using conventional enzymatic labeling methods. This step ensures relatively uniform labeling of the probe with primary amine groups. The aminoallyl dUTP substrate used in this reaction is taken up efficiently by reverse transcription or nick translation, for which we provide the protocols; other enzymatic methods are also likely to be compatible. In the second step, the amine-modified DNA is chemically labeled using an amine-reactive fluorescent dye. This chemical reaction varies little in its efficiency from dye to dye, so that it is possible to use any combination of the ARES Kits, with their broad selection of the brightest and most photostable dyes, and obtain consistent DNA labeling. The labeling protocols provided generally result in incorporation of about one dye per 12–15 bases, which we have determined to be optimal for fluorescence in situ hybridization (FISH) and dot-blot hybridization.

Each ARES DNA Labeling Kit provides sufficient reagents for 5 to 10 labelings of 1–5 µg DNA each, including:

  • 5-(3-Aminoallyl)-dUTP
  • Amine-reactive fluorescent dye and appropriate solvent
  • Sodium bicarbonate
  • Nuclease-free H2O
  • Detailed protocol for labeling DNA using reverse transcription or nick translation (ARES DNA Labeling Kits)

See Labeling Oligonucleotides and Nucleic Acids—Section 8.2 for a complete description of the ARES Kits, as well as of the FISH Tag DNA and FISH Tag RNA Kits, which employ the same aminoallyl nucleotide labeling method but provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each FISH Tag Kit provides all of the reagents needed for enzymatically incorporating the amine-modified nucleotide (aminoallyl dUTP or aminoallyl UTP) into DNA or RNA, followed by fluorescent labeling with an amine-reactive Alexa Fluor dye and purification of the labeled probe using PureLink nucleic acid purification technology (e.g., PureLink PCR Purification Kit, K3100-01). FISH Tag DNA and FISH Tag RNA Kits are each available as single-color kits containing one of four amine-reactive Alexa Fluor dyes or in a multicolor kit that contains all four reactive Alexa Fluor dyes.

ARES DNA Labeling Kits
Figure 1.2.11
Schematic diagram of the labeling method provided in our ARES DNA Labeling Kits. The ARES DNA Labeling Kits use a two-step method to label DNA. Step 1) The aminoallyl dUTP is enzymatically incorporated. Step 2) A reactive fluorophore is used to label the incorporated aminoallyl group.

Alexa Fluor Oligonucleotide Amine Labeling Kits

The Alexa Fluor Oligonucleotide Amine Labeling Kits (Labeling Oligonucleotides and Nucleic Acids—Section 8.2; Active esters and kits for labeling proteins and nucleic acids—Table 1.2, Molecular Probes kits for protein and nucleic acid labeling—Table 1.3) provide the reagents required for labeling synthetic oligonucleotides that have amine groups incorporated at their 5'-terminus. Following purification by standard chromatographic or electrophoretic procedures, these singly labeled oligonucleotides can serve as hybridization or ligation probes for a variety of applications.ref

Each Alexa Fluor Oligonucleotide Amine Labeling Kit contains sufficient reagents for three labelings of 50 µg each of an amine-modified oligonucleotide, including:

Biotin Quantitation Assay Kits

FluoReporter Biotin Quantitation Assay Kit for Biotinylated Proteins

The FluoReporter Biotin Quantitation Assay Kit for biotinylated proteins (F30751) provides a sensitive fluorometric assay for determining the number of biotin labels on a protein. This assay is based on the displacement of a ligand tagged with a quencher dye from the biotin-binding sites of Biotective Green reagent.ref The FluoReporter biotin quantitation assay can detect from 4 to 80 picomoles of biotin in a sample, providing a 50-fold higher sensitivity than the spectrophotometric HABA biotin-binding assay. Furthermore, unlike the HABA biotin-binding assay, which requires ~1 mg of protein sample, the FluoReporter biotin quantitation assay requires a minimum of 600 ng of a singly biotinylated IgG with molecular weight 150,000 daltons. For proteins of lower molecular weight or multiple biotin labels, less protein can be used. To expose any biotin groups in a multiply labeled protein that are sterically restricted and inaccessible to the Biotective Green reagent, this kit includes protease and an optional protocol for digesting the protein. With this preliminary digestion, biotin assay values agree well with MALDI-TOF determinations. With excitation/emission maxima of 495/519 nm, this assay is compatible with any fluorescence-based microplate reader capable of detecting fluorescein (FITC) or Alexa Fluor 488 dye; it can also be scaled up for fluorometer-based experiments.

Each FluoReporter Biotin Quantitation Assay Kit for biotinylated proteins includes:

Sufficient reagents are provided for assaying 5 samples independently using eight wells in triplicate for the standard curve and three dilutions of the sample in triplicate (totaling 33 wells per assay). However, fewer wells may be used to conserve sample and a single standard curve can be used for multiple samples in the same experimental session.

FluoReporter Biotin Quantitation Assay Kit for Biotinylated Nucleic Acids

The FluoReporter Biotin Quantitation Assay Kit for biotinylated nucleic acids (F30755) provides a sensitive fluorometric assay for determining the number of biotin labels on a nucleic acid. This assay is based on the displacement of a quencher dye from the biotin-binding sites of Biotective Green reagent. The FluoReporter biotin quantitation assay can detect from 4 to 80 picomoles of biotin in a sample, providing a 50-fold higher sensitivity than the spectrophotometric HABA biotin-binding assay. Analysis of multiply biotinylated nucleic acids requires a preliminary nuclease digestion step to avoid underestimation caused by steric restriction of avidin binding. This kit can be applied to as little as 13 ng of biotin-labeled nucleic acid and is ideal for determining the degree of biotinylation of cDNA samples used for microarray expression analysis.

Each FluoReporter Biotin Quantitation Assay Kit for biotinylated nucleic acids includes:

Sufficient reagents are provided for assaying 10 samples independently using eight wells in triplicate for the standard curve and three dilutions of the sample in triplicate (totaling 33 wells per assay). However, fewer wells may be used to conserve sample and a single standard curve can be used for multiple samples in the same experimental session.

All Kits for Labeling Proteins and Nucleic Acids—Ordering Information

Catalog # Name Size List Price (USD) Qty
A10170 Alexa Fluor® 350 Protein Labeling Kit 1 kit 394.00
A10171 Alexa Fluor® 430 Protein Labeling Kit 1 kit 472.00
A10235 Alexa Fluor® 488 Protein Labeling Kit 1 kit 394.00
A10236 Alexa Fluor® 532 Protein Labeling Kit 1 kit 423.00
A10237 Alexa Fluor® 546 Protein Labeling Kit 1 kit 394.00
A10238 Alexa Fluor® 568 Protein Labeling Kit 1 kit 394.00
A10239 Alexa Fluor® 594 Protein Labeling Kit 1 kit 394.00
A10468 APEX™ Alexa Fluor® 488 Antibody Labeling Kit 1 kit 389.00
A10470 APEX™ Alexa Fluor® 555 Antibody Labeling Kit 1 kit 389.00
A10474 APEX™ Alexa Fluor® 594 Antibody Labeling Kit 1 kit 389.00
A10475 APEX™ Alexa Fluor® 647 Antibody Labeling Kit 1 kit 389.00
A10476 APEX™ Oregon Green® 488 Antibody Labeling Kit 1 kit 386.00
A10478 APEX™ Pacific Blue™ Antibody Labeling Kit 1 kit 389.00
A10494 APEX™ Alexa Fluor® 568 Antibody Labeling Kit 1 kit 389.00
A10495 APEX™ Biotin-XX Antibody Labeling Kit 1 kit 389.00
A20170 Alexa Fluor® 633 Protein Labeling Kit 1 kit 394.00
A20171 Alexa Fluor® 660 Protein Labeling Kit 1 kit 394.00
A20172 Alexa Fluor® 680 Protein Labeling Kit 1 kit 394.00
A20173 Alexa Fluor® 647 Protein Labeling Kit 1 kit 394.00
A20174 Alexa Fluor® 555 Protein Labeling Kit 1 kit 394.00
A20180 Alexa Fluor® 350 Antibody Labeling Kit 1 kit 407.00
A20181 Alexa Fluor® 488 Antibody Labeling Kit 1 kit 407.00
A20182 Alexa Fluor® 532 Antibody Labeling Kit 1 kit 407.00
A20183 Alexa Fluor® 546 Antibody Labeling Kit 1 kit 407.00
A20184 Alexa Fluor® 568 Antibody Labeling Kit 1 kit 407.00
A20185 Alexa Fluor® 594 Antibody Labeling Kit 1 kit 407.00
A20186 Alexa Fluor® 647 Antibody Labeling Kit 1 kit 407.00
A20187 Alexa Fluor® 555 Antibody Labeling Kit 1 kit 407.00
A30006 Alexa Fluor® 488 Microscale Protein Labeling Kit 1 kit 411.00
A30007 Alexa Fluor® 555 Microscale Protein Labeling Kit 1 kit 411.00
A30008 Alexa Fluor® 594 Microscale Protein Labeling Kit 1 kit 411.00
A30009 Alexa Fluor® 647 Microscale Protein Labeling Kit 1 kit 411.00
B30010 Biotin-XX Microscale Protein Labeling Kit 1 kit 358.00
B30756 Biotin-XX Microscale Protein Labeling Kit with FluoReporter® Biotin Quantitation Assay Kit 1 kit 425.00
D20655 DSB-X™ Biotin Protein Labeling Kit 1 kit 387.00
F10240 Fluorescein-EX Protein Labeling Kit 1 kit 369.00
F6347 FluoReporter® Mini-Biotin-XX Protein Labeling Kit 1 kit 339.00
F6434 FluoReporter® FITC Protein Labeling Kit 1 kit 373.00
O10241 Oregon Green® 488 Protein Labeling Kit 1 kit 365.00
P30012 Pacific Blue™ Protein Labeling Kit 1 kit 420.00
P30013 Pacific Blue™ Antibody Labeling Kit 1 kit 420.00
P30014 Pacific Orange™ Antibody Labeling Kit 1 kit 435.00
P30016 Pacific Orange™ Protein Labeling Kit 1 kit 440.00
S30044 SAIVI™ Alexa Fluor® 647 Antibody/Protein 1 mg-Labeling Kit 1 kit 487.00
S30045 SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor® 680 1 kit 556.00
S30046 SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor® 750 1 kit 548.00
T10244 Texas Red®-X Protein Labeling Kit 1 kit 365.00
F30751 FluoReporter® Biotin Quantitation Assay Kit, for biotinylated proteins 1 kit 211.00
A20188 Alexa Fluor® 680 Antibody Labeling Kit 1 kit 348.24
A20189 Alexa Fluor® 790 Antibody Labeling Kit 1 kit 348.24
S10467 SiteClick™ R-PE Antibody Labeling Kit 1 kit 315.00
S10449 SiteClick™ Qdot® 525 Antibody Labeling Kit 1 kit 366.00
S10450 SiteClick™ Qdot® 565 Antibody Labeling Kit 1 kit 366.00
S10451 SiteClick™ Qdot® 585 Antibody Labeling Kit 1 kit 366.00
S10469 SiteClick™ Qdot® 605 Antibody Labeling Kit 1 kit 369.00
S10452 SiteClick™ Qdot® 625 Antibody Labeling Kit 1 kit 366.00
S10453 SiteClick™ Qdot® 655 Antibody Labeling Kit 1 kit 366.00
S10454 SiteClick™ Qdot® 705 Antibody Labeling Kit 1 kit 366.00
S10455 SiteClick™ Qdot® 800 Antibody Labeling Kit 1 kit 366.00
A20191 Alexa Fluor® 488 Oligonucleotide Amine Labeling Kit 1 kit 384.00
A20196 Alexa Fluor® 647 Oligonucleotide Amine Labeling Kit 1 kit 384.00
A21665 ARES™ Alexa Fluor® 488 DNA Labeling Kit 1 kit 362.00
A21667 ARES™ Alexa Fluor® 546 DNA Labeling Kit 1 kit 362.00
A21669 ARES™ Alexa Fluor® 594 DNA Labeling Kit 1 kit 362.00
A21676 ARES™ Alexa Fluor® 647 DNA Labeling Kit 1 kit 362.00
A21677 ARES™ Alexa Fluor® 555 DNA Labeling Kit 1 kit 362.00
F32947 FISH Tag™ DNA Green Kit, with Alexa Fluor® 488 dye 1 kit 378.00
F32948 FISH Tag™ DNA Orange Kit, with Alexa Fluor® 555 dye 1 kit 378.00
F32949 FISH Tag™ DNA Red Kit, with Alexa Fluor® 594 dye 1 kit 378.00
F32951 FISH Tag™ DNA Multicolor Kit, Alexa Fluor® dye combination 1 kit 419.00
F32952 FISH Tag™ RNA Green Kit, with Alexa Fluor® 488 dye 1 kit 453.00
F32954 FISH Tag™ RNA Red Kit, with Alexa Fluor® 594 dye 1 kit 453.00
F32956 FISH Tag™ RNA Multicolor Kit, Alexa Fluor® dye combination 1 kit 458.00
U21650 Ulysis™ Alexa Fluor® 488 Nucleic Acid Labeling Kit 1 kit 352.00
U21652 Ulysis™ Alexa Fluor® 546 Nucleic Acid Labeling Kit 1 kit 352.00
U21654 Ulysis™ Alexa Fluor® 594 Nucleic Acid Labeling Kit 1 kit 352.00
U21660 Ulysis™ Alexa Fluor® 647 Nucleic Acid Labeling Kit 1 kit 352.00
Z25000 Zenon® Alexa Fluor® 350 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25002 Zenon® Alexa Fluor® 488 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25003 Zenon® Alexa Fluor® 532 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25004 Zenon® Alexa Fluor® 546 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25005 Zenon® Alexa Fluor® 555 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25006 Zenon® Alexa Fluor® 568 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25007 Zenon® Alexa Fluor® 594 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25008 Zenon® Alexa Fluor® 647 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25010 Zenon® Alexa Fluor® 680 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25011 Zenon® Alexa Fluor® 700 Mouse IgG1 Labeling Kit 1 kit 311.00
Z25013 Zenon® Alexa Fluor® 405 Mouse IgG1 Labeling Kit 1 kit 311.00
Z25020 Zenon® Alexa Fluor® 610—R-Phycoerythrin Mouse IgG1 Labeling Kit 1 kit 321.00
Z25021 Zenon® Alexa Fluor® 647—R-Phycoerythrin Mouse IgG1 Labeling Kit 1 kit 342.00
Z25031 Zenon® Alexa Fluor® 750—Allophycocyanin Mouse IgG1 Labeling Kit 1 kit 310.00
Z25041 Zenon® Pacific Blue™ Mouse IgG1 Labeling Kit 1 kit 314.00
Z25042 Zenon® Fluorescein Mouse IgG1 Labeling Kit 1 kit 300.00
Z25051 Zenon® Allophycocyanin Mouse IgG1 Labeling Kit 1 kit 313.00
Z25052 Zenon® Biotin-XX Mouse IgG1 Labeling Kit 1 kit 300.00
Z25054 Zenon® Horseradish Peroxidase Mouse IgG1 Labeling Kit 1 kit 300.00
Z25055 Zenon® R-Phycoerythrin Mouse IgG1 Labeling Kit 1 kit 313.00
Z25060 Zenon® Tricolor Mouse IgG1 Labeling Kit #1 (For Green, Orange and Deep Red Fluorescence Imaging) 1 kit 300.00
Z25070 Zenon® Tricolor Mouse IgG1 Labeling Kit #2 (for Blue, Green and Red Fluorescence Imaging) 1 kit 300.00
Z25080 Zenon® Tricolor Mouse IgG1 Labeling Kit #3, for flow cytometry, 488 nm excitation 1 kit 466.00
Z25090 Zenon® Alexa Fluor® 488 Mouse IgG1 Labeling Kit, enhanced with TSA™ technology 1 kit 300.00
Z25100 Zenon® Alexa Fluor® 350 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25102 Zenon® Alexa Fluor® 488 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25105 Zenon® Alexa Fluor® 555 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25106 Zenon® Alexa Fluor® 568 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25107 Zenon® Alexa Fluor® 594 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25108 Zenon® Alexa Fluor® 647 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25110 Zenon® Alexa Fluor® 680 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25113 Zenon® Alexa Fluor® 405 Mouse IgG2a Labeling Kit 1 kit 335.00
Z25151 Zenon® Allophycocyanin Mouse IgG2a Labeling Kit 1 kit 314.00
Z25152 Zenon® Biotin-XX Mouse IgG2a Labeling Kit 1 kit 300.00
Z25154 Zenon® Horseradish Peroxidase Mouse IgG2a Labeling Kit 1 kit 300.00
Z25155 Zenon® R-Phycoerythrin Mouse IgG2a Labeling Kit 1 kit 314.00
Z25156 Zenon® Pacific Blue™ Mouse IgG2a Labeling Kit 1 kit 304.00
Z25160 Zenon® Tricolor Mouse IgG2a Labeling Kit #1 (for Green, Orange and Deep Red Fluorescence Imaging) 1 kit 300.00
Z25170 Zenon® Tricolor Mouse IgG2a Labeling Kit #2 (for Blue, Green and Red Fluorescence Imaging) 1 kit 300.00
Z25202 Zenon® Alexa Fluor® 488 Mouse IgG2b Labeling Kit 1 kit 300.00
Z25205 Zenon® Alexa Fluor® 555 Mouse IgG2b Labeling Kit 1 kit 300.00
Z25207 Zenon® Alexa Fluor® 594 Mouse IgG2b Labeling Kit 1 kit 300.00
Z25208 Zenon® Alexa Fluor® 647 Mouse IgG2b Labeling Kit 1 kit 300.00
Z25210 Zenon® Alexa Fluor® 680 Mouse IgG2b Labeling Kit 1 kit 300.00
Z25213 Zenon® Alexa Fluor® 405 Mouse IgG2b Labeling Kit 1 kit 365.00
Z25251 Zenon® Allophycocyanin Mouse IgG2b Labeling Kit 1 kit 300.00
Z25252 Zenon® Biotin-XX Mouse IgG2b Labeling Kit 1 kit 300.00
Z25254 Zenon® Horseradish Peroxidase Mouse IgG2b Labeling Kit 1 kit 300.00
Z25255 Zenon® R-Phycoerythrin Mouse IgG2b Labeling Kit 1 kit 314.00
Z25256 Zenon® Pacific Orange™ Mouse IgG1 Labeling Kit 1 kit 314.00
Z25257 Zenon® Pacific Orange™ Mouse IgG2a Labeling Kit 1 kit 319.00
Z25260 Zenon® Tricolor Mouse IgG2b Labeling Kit #1 (for Green, Orange and Deep Red Fluorescence Imaging) 1 kit 300.00
Z25300 Zenon® Alexa Fluor® 350 Rabbit IgG Labeling Kit 1 kit 300.00
Z25302 Zenon® Alexa Fluor® 488 Rabbit IgG Labeling Kit 1 kit 300.00
Z25303 Zenon® Alexa Fluor® 532 Rabbit IgG Labeling Kit 1 kit 300.00
Z25304 Zenon® Alexa Fluor® 546 Rabbit IgG Labeling Kit 1 kit 300.00
Z25305 Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit 1 kit 300.00
Z25306 Zenon® Alexa Fluor® 568 Rabbit IgG Labeling Kit 1 kit 300.00
Z25307 Zenon® Alexa Fluor® 594 Rabbit IgG Labeling Kit 1 kit 300.00
Z25308 Zenon® Alexa Fluor® 647 Rabbit IgG Labeling Kit 1 kit 300.00
Z25312 Zenon® Alexa Fluor® 750 Rabbit IgG Labeling Kit 1 kit 300.00
Z25313 Zenon® Alexa Fluor® 405 Rabbit IgG Labeling Kit 1 kit 310.00
Z25342 Zenon® Fluorescein Rabbit IgG Labeling Kit 1 kit 300.00
Z25351 Zenon® Allophycocyanin Rabbit IgG Labeling Kit 1 kit 335.00
Z25352 Zenon® Biotin-XX Rabbit IgG Labeling Kit 1 kit 300.00
Z25354 Zenon® Horseradish Peroxidase Rabbit IgG Labeling Kit 1 kit 300.00
Z25355 Zenon® R-Phycoerythrin Rabbit IgG Labeling Kit 1 kit 310.00
Z25360 Zenon® Tricolor Rabbit IgG Labeling Kit #1 (for Green, Orange and Deep Red Fluorescence Imaging) 1 kit 300.00
Z25370 Zenon® Tricolor Rabbit IgG Labeling Kit #2 (for Blue, Green and Red Fluorescence Imaging) 1 kit 300.00
Z25402 Zenon® Alexa Fluor® 488 Human IgG Labeling Kit 1 kit 300.00
Z25407 Zenon® Alexa Fluor® 594 Human IgG Labeling Kit 1 kit 300.00
Z25408 Zenon® Alexa Fluor® 647 Human IgG Labeling Kit 1 kit 300.00
Z25451 Zenon® Allophycocyanin Human IgG Labeling Kit 1 kit 310.00
Z25452 Zenon® Biotin-XX Human IgG Labeling Kit 1 kit 300.00
Z25454 Zenon® Horseradish Peroxidase Human IgG Labeling Kit 1 kit 300.00
Z25455 Zenon® R-Phycoerythrin Human IgG Labeling Kit 1 kit 319.00
Z25602 Zenon® Alexa Fluor® 488 Goat IgG Labeling Kit - 50 Labeling 1 kit 300.00
Z25605 Zenon® Alexa Fluor® 555 Goat IgG Labeling Kit - 50 Labeling 1 kit 300.00
Z25607 Zenon® Alexa Fluor® 594 Goat IgG Labeling Kit - 50 Labeling 1 kit 300.00
Z25608 Zenon® Alexa Fluor® 647 Goat IgG Labeling Kit - 50 Labeling 1 kit 300.00

Protein Labeling Kits—Ordering Information

Catalog # Name Size List Price (USD) Qty
A10170 Alexa Fluor® 350 Protein Labeling Kit 1 kit 394.00
A10171 Alexa Fluor® 430 Protein Labeling Kit 1 kit 472.00
A10235 Alexa Fluor® 488 Protein Labeling Kit 1 kit 394.00
A10236 Alexa Fluor® 532 Protein Labeling Kit 1 kit 423.00
A10237 Alexa Fluor® 546 Protein Labeling Kit 1 kit 394.00
A10238 Alexa Fluor® 568 Protein Labeling Kit 1 kit 394.00
A10239 Alexa Fluor® 594 Protein Labeling Kit 1 kit 394.00
A10468 APEX™ Alexa Fluor® 488 Antibody Labeling Kit 1 kit 389.00
A10470 APEX™ Alexa Fluor® 555 Antibody Labeling Kit 1 kit 389.00
A10474 APEX™ Alexa Fluor® 594 Antibody Labeling Kit 1 kit 389.00
A10475 APEX™ Alexa Fluor® 647 Antibody Labeling Kit 1 kit 389.00
A10476 APEX™ Oregon Green® 488 Antibody Labeling Kit 1 kit 386.00
A10478 APEX™ Pacific Blue™ Antibody Labeling Kit 1 kit 389.00
A10494 APEX™ Alexa Fluor® 568 Antibody Labeling Kit 1 kit 389.00
A10495 APEX™ Biotin-XX Antibody Labeling Kit 1 kit 389.00
A20170 Alexa Fluor® 633 Protein Labeling Kit 1 kit 394.00
A20171 Alexa Fluor® 660 Protein Labeling Kit 1 kit 394.00
A20172 Alexa Fluor® 680 Protein Labeling Kit 1 kit 394.00
A20173 Alexa Fluor® 647 Protein Labeling Kit 1 kit 394.00
A20174 Alexa Fluor® 555 Protein Labeling Kit 1 kit 394.00
A20180 Alexa Fluor® 350 Antibody Labeling Kit 1 kit 407.00
A20181 Alexa Fluor® 488 Antibody Labeling Kit 1 kit 407.00
A20182 Alexa Fluor® 532 Antibody Labeling Kit 1 kit 407.00
A20183 Alexa Fluor® 546 Antibody Labeling Kit 1 kit 407.00
A20184 Alexa Fluor® 568 Antibody Labeling Kit 1 kit 407.00
A20185 Alexa Fluor® 594 Antibody Labeling Kit 1 kit 407.00
A20186 Alexa Fluor® 647 Antibody Labeling Kit 1 kit 407.00
A20187 Alexa Fluor® 555 Antibody Labeling Kit 1 kit 407.00
A30006 Alexa Fluor® 488 Microscale Protein Labeling Kit 1 kit 411.00
A30007 Alexa Fluor® 555 Microscale Protein Labeling Kit 1 kit 411.00
A30008 Alexa Fluor® 594 Microscale Protein Labeling Kit 1 kit 411.00
A30009 Alexa Fluor® 647 Microscale Protein Labeling Kit 1 kit 411.00
B30010 Biotin-XX Microscale Protein Labeling Kit 1 kit 358.00
B30756 Biotin-XX Microscale Protein Labeling Kit with FluoReporter® Biotin Quantitation Assay Kit 1 kit 425.00
D20655 DSB-X™ Biotin Protein Labeling Kit 1 kit 387.00
F10240 Fluorescein-EX Protein Labeling Kit 1 kit 369.00
F6347 FluoReporter® Mini-Biotin-XX Protein Labeling Kit 1 kit 339.00
F6434 FluoReporter® FITC Protein Labeling Kit 1 kit 373.00
O10241 Oregon Green® 488 Protein Labeling Kit 1 kit 365.00
P30012 Pacific Blue™ Protein Labeling Kit 1 kit 420.00
P30013 Pacific Blue™ Antibody Labeling Kit 1 kit 420.00
P30014 Pacific Orange™ Antibody Labeling Kit 1 kit 435.00
P30016 Pacific Orange™ Protein Labeling Kit 1 kit 440.00
S30044 SAIVI™ Alexa Fluor® 647 Antibody/Protein 1 mg-Labeling Kit 1 kit 487.00
S30045 SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor® 680 1 kit 556.00
S30046 SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor® 750 1 kit 548.00
T10244 Texas Red®-X Protein Labeling Kit 1 kit 365.00
F30751 FluoReporter® Biotin Quantitation Assay Kit, for biotinylated proteins 1 kit 211.00
A20188 Alexa Fluor® 680 Antibody Labeling Kit 1 kit 348.24
A20189 Alexa Fluor® 790 Antibody Labeling Kit 1 kit 348.24
S10467 SiteClick™ R-PE Antibody Labeling Kit 1 kit 315.00
S10449 SiteClick™ Qdot® 525 Antibody Labeling Kit 1 kit 366.00
S10450 SiteClick™ Qdot® 565 Antibody Labeling Kit 1 kit 366.00
S10451 SiteClick™ Qdot® 585 Antibody Labeling Kit 1 kit 366.00
S10469 SiteClick™ Qdot® 605 Antibody Labeling Kit 1 kit 369.00
S10452 SiteClick™ Qdot® 625 Antibody Labeling Kit 1 kit 366.00
S10453 SiteClick™ Qdot® 655 Antibody Labeling Kit 1 kit 366.00
S10454 SiteClick™ Qdot® 705 Antibody Labeling Kit 1 kit 366.00
S10455 SiteClick™ Qdot® 800 Antibody Labeling Kit 1 kit 366.00

Nucleic Acid Labeling Kits—Ordering Information

Zenon Antibody Labeling Kits—Ordering Information

Catalog # Name Size List Price (USD) Qty
Z25000 Zenon® Alexa Fluor® 350 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25002 Zenon® Alexa Fluor® 488 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25003 Zenon® Alexa Fluor® 532 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25004 Zenon® Alexa Fluor® 546 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25005 Zenon® Alexa Fluor® 555 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25006 Zenon® Alexa Fluor® 568 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25007 Zenon® Alexa Fluor® 594 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25008 Zenon® Alexa Fluor® 647 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25010 Zenon® Alexa Fluor® 680 Mouse IgG1 Labeling Kit 1 kit 300.00
Z25011 Zenon® Alexa Fluor® 700 Mouse IgG1 Labeling Kit 1 kit 311.00
Z25013 Zenon® Alexa Fluor® 405 Mouse IgG1 Labeling Kit 1 kit 311.00
Z25020 Zenon® Alexa Fluor® 610—R-Phycoerythrin Mouse IgG1 Labeling Kit 1 kit 321.00
Z25021 Zenon® Alexa Fluor® 647—R-Phycoerythrin Mouse IgG1 Labeling Kit 1 kit 342.00
Z25031 Zenon® Alexa Fluor® 750—Allophycocyanin Mouse IgG1 Labeling Kit 1 kit 310.00
Z25041 Zenon® Pacific Blue™ Mouse IgG1 Labeling Kit 1 kit 314.00
Z25042 Zenon® Fluorescein Mouse IgG1 Labeling Kit 1 kit 300.00
Z25051 Zenon® Allophycocyanin Mouse IgG1 Labeling Kit 1 kit 313.00
Z25052 Zenon® Biotin-XX Mouse IgG1 Labeling Kit 1 kit 300.00
Z25054 Zenon® Horseradish Peroxidase Mouse IgG1 Labeling Kit 1 kit 300.00
Z25055 Zenon® R-Phycoerythrin Mouse IgG1 Labeling Kit 1 kit 313.00
Z25060 Zenon® Tricolor Mouse IgG1 Labeling Kit #1 (For Green, Orange and Deep Red Fluorescence Imaging) 1 kit 300.00
Z25070 Zenon® Tricolor Mouse IgG1 Labeling Kit #2 (for Blue, Green and Red Fluorescence Imaging) 1 kit 300.00
Z25080 Zenon® Tricolor Mouse IgG1 Labeling Kit #3, for flow cytometry, 488 nm excitation 1 kit 466.00
Z25090 Zenon® Alexa Fluor® 488 Mouse IgG1 Labeling Kit, enhanced with TSA™ technology 1 kit 300.00
Z25100 Zenon® Alexa Fluor® 350 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25102 Zenon® Alexa Fluor® 488 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25105 Zenon® Alexa Fluor® 555 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25106 Zenon® Alexa Fluor® 568 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25107 Zenon® Alexa Fluor® 594 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25108 Zenon® Alexa Fluor® 647 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25110 Zenon® Alexa Fluor® 680 Mouse IgG2a Labeling Kit 1 kit 300.00
Z25113 Zenon® Alexa Fluor® 405 Mouse IgG2a Labeling Kit 1 kit 335.00