Table 1.2 Active esters and kits for labeling proteins and nucleic acids.

Label Fluorescence Color (Abs/Em) * Reactive** Dye or Biotin Protein Labeling Kits Zenon Antibody Labeling Kits Nucleic Acid Labeling Kits
Alexa Fluor 350 Blue
(346/442)
A10168 A20180 (Ab)
A10170 (P)
Z25000 (M IgG1)
Z25100 (M IgG2a)
Z25300 (R IgG)
 
Marina Blue Blue
(365/460)
M10165      
Pacific Orange Orange
(400/551)
P30253 P30014 (Ab)
P30016 (P)
Z25256 (M IgG1)
Z25257 (M IgG2a)
 
Alexa Fluor 405 Blue
(402/421)
A30000
A30100
  Z25013 (M IgG1)
Z25113 (M IgG2a)
Z25213 (M IgG2b)
Z25313 (R IgG)
 
Pacific Blue Blue
(410/455)
P10163 A10478 (APEX)
P30013 (Ab)
P30012 (P)
Z25041 (M IgG1)
Z25156 (M IgG2a)
Z25341 (R IgG)
 
Alexa Fluor 430 Yellow-green
(434/539)
A10169 A10171 (P) Z25001 (M IgG1)  
Fluorescein-EX Green
(494/518)
F6130 F10240 (P)
F6433 (F)
Z25042 (M IgG1)
Z25342 (R IgG)
C7604 (Nuc)
F32767 (Nuc)
FITC Green
(494/518)
F143  (I)
F1906 (I)
F1907 (I)
F6434 (F)    
Alexa Fluor 488 Green
(495/519)

A20000 
A20100 
A30052 (SDP)
A30005 (TFP)
A37563 (TFP)
A37570 (TFP)

A10468  (APEX)
A30006  (Micro)
A20181  (Ab)
A10235  (P)
Z25002 (M IgG1)
Z25090 (TSA) (M IgG1)
Z25102 (M IgG2a)
Z25202 (M IgG2b)
Z25302 (R IgG)
Z25602 (G IgG)
Z25402 (H IgG)
U21650 (ULYSIS)
A21665 (ARES)
F32947 (FT)
F32952 (FT)
A20191 (Oligo)
A32750 (Deca)
C11397 (Nuc)
C11403 (Nuc)

 

Oregon Green 488 Green
(496/524)
O6080 (I) O6147
O6149
06185 (X)
A10476 (APEX)
O10241 (P)
   
pHrodo Green Green
(505/525)
P35369 (STP)      
Oregon Green 514 Green
(511/530)
O6139      
Alexa Fluor 514 Yellow-green
(518/540)
A30002       
Alexa Fluor 532 Yellow
(531/554)
A20001
A20101MP
A20182 (Ab)
A10236 (P)
Z25003 (M IgG1)
Z25303 (R IgG)
U21651 (ULYSIS)
C11398 (Nuc)
Alexa Fluor 546 Orange
(556/573)
A20002 
A20102 
A20183 (Ab)
A10237 (P)
Z25004 (M IgG1)
Z25104 (M IgG2a)
Z25204 (M IgG2b)
Z25304 (R IgG)

U21652 (ULYSIS)
A21667 (ARES)
C11401 (Nuc)
C11404 (Nuc)

Alexa Fluor 555 Red-orange (555/565) A20009
A20109
A37564
A37571
A10470 (APEX)
A30007 (Micro)
A20187 (Ab)
A20174 (P)
Z25005 (M IgG1)
Z25105 (M IgG2a)
Z25205 (M IgG2b)
Z25305 (R IgG)
Z25605 (G IgG)
A21677 (ARES)
F32948 (FT)
A32762 (Nuc)
A32770 (Nuc)
A32755 (Deca)
A32756 (Deca)

 

Tetramethylrhodamine

and TAMRA

Red-orange (555/580) T1480 (I)
T1481 (I) C2211
C6123
C1171 
T6105  (X)
     
pHrodo Red Red-orange
(560/587)
P36600 A10026 (PL)
P35363 (Micro)
   
Rhodamine Red Red-orange (570/590) R6160 (X)      
Alexa Fluor 568 Red-orange (578/603) A20003 
A20103 
A10494 (APEX)
A20184 (Ab)
A10238 (P)
Z25006 (M IgG1)
Z25106 (M IgG2a)
Z25206 (M IgG2b)
Z25306 (R IgG)
C11399 (Nuc)
Alexa Fluor 594 Red
(590/617)
A20004 
A20104 
A37565 
A37572
A10474 (APEX)
A30008 (Micro)
A20185 (Ab)
A10239 (P)
Z25007 (M IgG1)
Z25107 (M IgG2a)
Z25207 (M IgG2b)
Z25307 (R IgG)
Z25607 (G IgG)
Z25407 (H IgG)

U21654 (ULYSIS)
A21669 (ARES)
F32949 (FT)
F32954 (FT)
C11400 (Nuc)

Texas Red Red
(595/615)
T6134  (X)
T20175 (X)
T10244 (P) Z25045 (M IgG1) C7631 (Nuc)
Alexa Fluor 610 Red
(612/628)
A30050 (X)      
Alexa Fluor 633 § Far-red
(632/647)
A20005 
A20105 
A20170 (P)    
Alexa Fluor 635 § Far-red
(633/647)
       
Alexa Fluor 647 § Far-red
(650/668)
A20006
A20106
A37566
A37573
A10475 (APEX)
A30009 (Micro)
A20186 (Ab)
S30044 (SAIVI)
A20173 (P)
Z25008 (M IgG1)
Z25108 (M IgG2a)
Z25208 (M IgG2b)
Z25308 (R IgG)
Z25608 (G IgG)
Z25408 (H IgG)
U21660 (ULYSIS)
A21676 (ARES)
A20196 (Oligo)
A32763 (Nuc)
A32771 (Nuc)
A32757 (Deca)

 

Alexa Fluor 660 § Near-infrared
(663/690)
A20007 A20171 (P)    
Alexa Fluor 680 § Near-infrared (679/702) A20008
A20108
A37567
A37574
A20188 (Ab)
S30045 (SAIVI)
A20172 (P)
Z25010 (M IgG1)
Z25110 (M IgG2a)
Z25210 (M IgG2b)
Z25310 (R IgG)
 
Alexa Fluor 700 § Near-infrared
(702/723)
A20010
A20110
  Z25011 (M IgG1)  
Alexa Fluor 750 § Near-infrared (749/775) A20011
A20111
A37568
A37575
S30046 (SAIVI) Z25312 (R IgG)  
Alexa Fluor 790 § Near-infrared (782/805) A30051
A37569
A20189 (Ab)    
Biotin NA

B1513
B1582 (X)
B1606 (XX)
B6353 (S)(X)
B6352 (S)(XX)

A10495 (APEX)
B30010 (Micro)
B30756 (Micro)
F6347 (F)
Z25052 (M IgG1)
Z25152 (M IgG2a)
Z25252 (M IgG2b)
Z25352 (R IgG)
Z25452 (H IgG)
 
DNP–biotin NA (364/none) B2604 (X)      
DSB-X biotin NA   D20655 (P)    
* Approximate absorption (Abs) and fluorescence emission (Em) maxima for conjugates, in nm. ** Reactive Dye or Biotin has the succinimidyl ester (SE, NHS ester) group unless noted otherwise. § Human vision is insensitive to light beyond ~650 nm, and therefore it is not possible to view the far-red– and near-infrared–fluorescent dyes by looking through the eyepiece of a conventional fluorescence microscope. NA = Not applicable.

(Ab) = Antibody Labeling Kit
(APEX) = APEX™ Antibody Labeling Kit.
(ARES) = ARES™ DNA Labeling Kit.
(Deca) = Alexa Fluor® decapack, for microarrays.
(F) = FluoReporter Protein Labeling Kit.
(FT) = FISH Tag DNA Kit or FISH Tag RNA Kit.
(G IgG) = Zenon® Goat IgG Labeling Kit.
(H IgG) = Zenon® Human IgG Labeling Kit.
(I) = Isothiocyanate
(M IgG) = Zenon® Mouse IgG Labeling Kit.
(Micro) = Microscale Protein Labeling Kit.
(Nuc) = Nucleotides
(Oligo) = Alexa Fluor® Oligonucleotide Amine Labeling Kit.
(P) = Protein Labeling Kit.
(PL) = pHrodo™ Labeling Kit
(R IgG) = Zenon® Rabbit IgG Labeling Kit.
(S) = Sulfosuccinimidyl ester
(SAIVI) = SAIVI™ Antibody Labeling Kit.
(SDP) = Sulfodichlorophenol ester.
(STP) =  Sulfotetrafluorophenol ester
(TFP) = Tetrafluorophenyl ester.
(TSA) = Enhanced with TSA™ technology.
(ULYSIS) = ULYSIS® Nucleic Acid Labeling Kit.
(X) = An aminohexanoyl spacer between the dye and the SE.

(XX) = Two aminohexanoyl linkers conjugated to provide a 14-atom spacer between the dye and SE.

For Research Use Only. Not for use in diagnostic procedures.