Table 1.4 Alexa Fluor active esters and kits for labeling proteins and nucleic acids.

Alexa Fluor Dye Fluorescence Color (Abs/Em) * Succinimidyl Ester (NHS Ester),
TFP Ester
or SDP Ester
Kits for Labeling Proteins

Kits for Labeling Nucleic Acids and Oligonucleotides
Protein Labeling Kits Antibody Labeling Kits Zenon Antibody Labeling Kits
Alexa Fluor 350 Blue (346/442) A10168 A10170 (P) A20180 (Ab) Z25000 
(M IgG1)

Z25100 
(M IgG2a)

Z25200 
(M IgG2b)

Z25300 
(R IgG)

Z25400 
(H IgG)
 
Alexa Fluor 405 Blue (402/421) A30000

A30100
    Z25013 
(M IgG1)

Z25113 
(M IgG2a)

Z25213 
(M IgG2b)

Z25313
 (R IgG)
 
Alexa Fluor 430 Yellow-green (434/539) A10169 A10171 (P)   Z25001 
(M IgG1)

Z25301 
(R IgG)
 
Alexa Fluor 488 Green (495/519) A20000 †

A20100 †

A30052 (SDP)

A30005 (TFP)

A37563 (TFP)

A37570 (TFP)
A10235 (P) ‡

A30006 (Micro) ‡
A10468 (APEX) ‡

A20181 (Ab) ‡
Z25002 
(M IgG1)

Z25090 (TSA)
(M IgG1)

Z25102 
(M IgG2a)

Z25202 
(M IgG2b)

Z25302 
(R IgG)

Z25602 
(G IgG)

Z25402 
(H IgG)
U21650 (ULYSIS)

A21665 (ARES)

F32947 (FT)

F32952 (FT)

A20191 (Oligo)
Alexa Fluor 514 Yellow-green (518/540) A30002 †        
Alexa Fluor 532 Yellow (531/554) A20001

A20101MP
A10236 (P) A20182 (Ab) Z25003 
(M IgG1)

Z25303 
(R IgG)
U21651 (ULYSIS)
Alexa Fluor 546 Orange (556/573) A20002 †

A20102 †
A10237 (P) A20183 (Ab) Z25004 
(M IgG1)

Z25104 
(M IgG2a)

Z25204 
(M IgG2b)

Z25304 
(R IgG)
U21652 (ULYSIS)

A21667 (ARES)
Alexa Fluor 555 Red-orange (555/565) A20009

A20109

A37564

A37571
A20174 (P)

A30007 (Micro)
A10470 (APEX)

A20187 (Ab)
Z25005 
(M IgG1)

Z25105 
(M IgG2a)

Z25205 
(M IgG2b)

Z25305 
(R IgG)

Z25605 
(G IgG)

Z25405 
(H IgG)
A21677 (ARES)

F32948 (FT)

F32953 (FT)
Alexa Fluor 568 Red-orange (578/603) A20003 †

A20103 †
A10238 (P) A10494 (APEX)

A20184 (Ab)
Z25006 
(M IgG1)

Z25106 
(M IgG2a)

Z25206 
(M IgG2b)

Z25306 
(R IgG)

Z25606 (
G IgG)
U21653 (ULYSIS)
Alexa Fluor 594 Red (590/617) A20004 †

A20104 †

A37565 †

A37572 †
A10239 (P)

A30008 (Micro)
A10474 (APEX)

A20185 (Ab)

Z25007 
(M IgG1)

Z25107 
(M IgG2a)

Z25207 
(M IgG2b)

Z25307 
(R IgG)

Z25607 
(G IgG)

Z25407 
(H IgG)

U21654 (ULYSIS)

A21669 (ARES)

F32949 (FT)

F32954 (FT)
Alexa Fluor 610 Red (612/628) A30050 (X)        
Alexa Fluor 633 § Far-red (632/647) A20005 †

A20105 †
A20170 (P)      
Alexa Fluor 647 § Far-red (650/668) A20006

A20106

A37566

A37573
A20173 (P)

A30009 (Micro)
A10475 (APEX)

A20186 (Ab)

S30044 (SAIVI)
Z25008 
(M IgG1)

Z25108 
(M IgG2a)

Z25208 
(M IgG2b)

Z25308 
(R IgG)

Z25608 
(G IgG)

Z25408 
(H IgG)
U21660 (ULYSIS)

A21676 (ARES)

F32950 (FT)

F32955 (FT)

A20196 (Oligo)
Alexa Fluor 660 § Near-infrared (663/690) A20007 A20171 (P)   Z25009 
(M IgG1)
U21656 (ULYSIS)

A21671 (ARES)
Alexa Fluor 680 § Near-infrared (679/702) A20008

A20108

A37567

A37574
A20172 (P) A20188 (Ab)

S30045 (SAIVI)
Z25010 
(M IgG1)

Z25110 
(M IgG2a)

Z25210 
(M IgG2b)

Z25310 
(R IgG)
 
Alexa Fluor 700 § Near-infrared (702/723) A20010

A20110
    Z25011 
(M IgG1)
 
Alexa Fluor 750 § Near-infrared (749/775) A20011

A20111

A37568

A37575
  S30046 (SAIVI) Z25312 
(R IgG)
 
Alexa Fluor 790 § Near-infrared (782/805) A30051

A37569
  A20189 (Ab)    
* Approximate absorption (Abs) and fluorescence emission (Em) maxima for conjugates, in nm. † Mixed isomers. ‡ The APEX Alexa Fluor 488 Antibody Labeling Kits contain the Alexa Fluor 488 carboxylic acid SDP ester, whereas the other Alexa Fluor 488 protein labeling kits contain the amine-reactive Alexa Fluor 488 carboxylic acid TFP ester; the Alexa Fluor 488 kits for labeling nucleic acids and oligonucleotides contain the Alexa Fluor 488 succinimidyl ester. The Alexa Fluor Labeling Kits are described in detail in Kits for Labeling Proteins and Nucleic Acids—Section 1.2. § Human vision is insensitive to light beyond ~650 nm, and therefore it is not possible to view the far-red– and near-infrared–fluorescent dyes by looking through the eyepiece of a conventional fluorescence microscope.

(Ab) = Antibody Labeling Kit.
(APEX) = APEX Antibody Labeling Kit.
(ARES) = ARES DNA Labeling Kit.
(FT) = FISH Tag DNA Kit or FISH Tag RNA Kit.
(G IgG) = Zenon Goat IgG Labeling Kit.
(H IgG) = Zenon Human IgG Labeling Kit.
(M IgG) = Zenon Mouse IgG Labeling Kit.
(Micro) = Alexa Fluor Microscale Protein Labeling Kit.
(Oligo) = Alexa Fluor Oligonucleotide Amine Labeling Kit.
(P) = Easy-to-Use Protein Labeling Kit.
(R IgG) = Zenon Rabbit IgG Labeling Kit.
(SAIVI) = SAIVI Antibody Labeling Kit.
(SDP) = Sulfodichlorophenol ester.
(TFP) = Tetrafluorophenyl ester.
(TSA) = Enhanced with TSA technology.
(ULYSIS) = ULYSIS Nucleic Acid Labeling Kit.
(X) = An aminohexanoyl spacer between the dye and the SE.

 

For Research Use Only. Not for use in diagnostic procedures.