Table 8.7 Amine-reactive dyes for nucleic acid sequencing.

Cat. No. Reactive Dye * Ex/Em § Handbook Location
C2210 5-FAM, SE † 494/518 Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
C6164 6-FAM, SE 494/518 Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
C6127 5-CR 6G, SE 525/555 Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C6171MP 6-JOE, SE † 522/550 Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
C2211 5-TAMRA, SE 555/580 Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C6123 6-TAMRA, SE † 555/580 Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C6125 5-ROX, SE 580/605 Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C6126 6-ROX, SE † 580/605 Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
D2184 BODIPY FL, SE ‡ 505/513 BODIPY Dye Series—Section 1.4
D6140 BODIPY FL, SSE 505/513 BODIPY Dye Series—Section 1.4
D6102 BODIPY FL-X, SE 505/513 BODIPY Dye Series—Section 1.4
D6180 BODIPY R6G, SE ‡ 528/550 BODIPY Dye Series—Section 1.4
D6117 BODIPY TMR-X, SE 542/574 BODIPY Dye Series—Section 1.4
D2222 BODIPY 564/570, SE ‡ 565/571 BODIPY Dye Series—Section 1.4
D2228 BODIPY 581/591, SE ‡ 584/592 BODIPY Dye Series—Section 1.4
D6116 BODIPY TR-X, SE 589/617 BODIPY Dye Series—Section 1.4
* FAM = carboxyfluorescein; CR 6G = carboxyrhodamine 6G; JOE = 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein; TAMRA = carboxytetramethylrhodamine; ROX = carboxy-X-rhodamine; BODIPY = a substituted 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene derivative; SE = succinimidyl ester; SSE = water-soluble sulfosuccinimidyl ester. † These are the most widely used isomers for DNA sequencing.ref ‡ These BODIPY derivatives were reported to be useful for automated DNA sequencing, in part because the dyes have a minimal effect on the mobility of the fragment during electrophoresis and also exhibit well-resolved spectra with narrow bandwidths.ref § Excitation (Ex) and emission (Em) maxima in nm.
For Research Use Only. Not for use in diagnostic procedures.