Table 7.6 Binding profiles of protein A and protein G.

Antibody
Protein A
Protein G
Bovine
+
++
Cat
++
Chicken
Dog
++
+
Goat
+
++
Guinea pig
+
++
Horse
++
Human IgG1, IgG2, IgG4
++
++
Human IgG3
++
Human IgM, IgA, IgE
++
Human IgD
Mouse IgG1
++
Mouse (others)
++
++
Pig
++
++
Rabbit
++
++
Rat
+
Sheep
++
+ Moderate binding. ++ Strong binding. – Weak or no binding.
For Research Use Only. Not for use in diagnostic procedures.