Target Targeting Sequence Ref GFP
(489/508 nm)*
RFP
(555/584 nm)*
Handbook Section
ActinHuman actin1C10506 †
C10582
C10502 †
C10583
Section 11.1
TubulinHuman tubulin2C10509 †
C10613
C10503 †
C10614
Section 11.2
MAP4MAP43C10598C10599Section 11.2
TalinHuman talin 2341–25414C10611C10612Section 11.2
ChromatinHistone 2B (H2B)5C10594C10595Section 12.5
MitochondriaLeader sequence of E1α pyruvate dehydrogenase6C10508 †
C10600
C10505 †
C10601
Section 12.2
LysosomesLamp1 (lysosomal-associated membrane protein 1)7C10507 †
C10596
C10504 †
C10597
Section 12.3
PeroxisomesPeroxisomal C-terminal targeting sequence8C10604C10605Section 12.3
Early endosomesRab5a9C10586C10587Section 12.3, Section 16.1
Late endosomesRab7a9C10588C10589Section 12.3, Section 16.1
SynaptosomesSynaptophysin10C10609C10610Section 16.1
Endoplasmic reticulumER signal sequence of calreticulin and KDEL (ER retention signal)11C10590C10591Section 12.4
Golgi apparatusHuman golgi-resident enzyme N-acetylgalactosaminyltransferase 212C10592C10593Section 12.4
NucleusLSV40 nuclear localization sequence13C10602 ‡C10603 ‡Section 12.5
Plasma membraneMyristoylation/palmitoylation sequence from Lck tyrosine kinase14C10607 ‡C10608 ‡Section 14.4
CytoplasmNo targeting sequence
(BacMam GFP transduction control)**
 B10383 Section 14.7
* Approximate absorption (Abs) and fluorescence (Em) maxima, in nm; GFP (Green Fluorescent Protein) and RFP ref (Red Fluorescent Protein) can be imaged using optical filters for fluorescein (FITC) and tetramethylrhodamine (TRITC) dyes, respectively. † These popular CellLight reagents are offered in small unit sizes (100 µL); all others are provided in a unit size of 1 mL. ‡ Also available is CellLight Nucleus-CFP (C10616) and CellLight Plasma Membrane-CFP (C10606), which generate a cyan-autofluorescent fusion protein targeted to the nucleus and plasma membrane, respectively. ** We also offer the CellLight Null (control) reagent (C10615), a negative transduction control for our CellLight targeted fluorescent reagents that comprises a suspension of baculovirus particles lacking mammalian genetic elements. 1. Curr Biol (1997) 7:176; 2. PLoS One (2009) 4:e8171; 3. J Cell Biol (1995) 130:639; 4. Plant J (2003) 33:775; 5. Curr Biol (1998) 8:377; 6. J Biol Chem (2004) 279:13044; 7. J Cell Sci (2005) 118:5243; 8. J Cell Biol (1989) 108:1657; 9. J Biol Chem (2009) 284:29218; 10. J Neurosci (2006) 26:3604; 11. FEBS Lett (1997) 405:18; 12. J Cell Biol (1998) 143:1505; 13. Trends Biochem Sci (1991) 16:478; 14. EMBO J (1997) 16:4983.
For Research Use Only. Not for use in diagnostic procedures.