Table 11.1 CellLight reagents and their targeting sequences.

Target Targeting Sequence Ref GFP
(489/508 nm)*
RFP
(555/584 nm)*
Handbook Section
Actin Human actin 1 C10506 †
C10582
C10502 †
C10583
Section 11.1
Tubulin Human tubulin 2 C10509 †
C10613
C10503 †
C10614
Section 11.2
MAP4 MAP4 3   C10599 Section 11.2
Talin Human talin 2341–2541 4 C10611 C10612 Section 11.2
Chromatin Histone 2B (H2B) 5 C10594 C10595 Section 12.5
Mitochondria Leader sequence of E1α pyruvate dehydrogenase 6 C10508 †
C10600
C10505 †
C10601
Section 12.2
Lysosomes Lamp1 (lysosomal-associated membrane protein 1) 7 C10507 †
C10596
C10504 †
C10597
Section 12.3
Peroxisomes Peroxisomal C-terminal targeting sequence 8 C10604 C10605 Section 12.3
Early endosomes Rab5a 9 C10586 C10587 Section 12.3, Section 16.1
Late endosomes Rab7a 9 C10588 C10589 Section 12.3, Section 16.1
Synaptosomes Synaptophysin 10   C10610 Section 16.1
Endoplasmic reticulum ER signal sequence of calreticulin and KDEL (ER retention signal) 11 C10590 C10591 Section 12.4
Golgi apparatus Human golgi-resident enzyme N-acetylgalactosaminyltransferase 2 12 C10592 C10593 Section 12.4
Nucleus LSV40 nuclear localization sequence 13 C10602 ‡ C10603 ‡ Section 12.5
Plasma membrane Myristoylation/palmitoylation sequence from Lck tyrosine kinase 14 C10607 ‡ C10608 ‡ Section 14.4
Cytoplasm No targeting sequence
(BacMam GFP transduction control)**
  B10383   Section 14.7
* Approximate absorption (Abs) and fluorescence (Em) maxima, in nm; GFP (Green Fluorescent Protein) and RFP ref (Red Fluorescent Protein) can be imaged using optical filters for fluorescein (FITC) and tetramethylrhodamine (TRITC) dyes, respectively. † These popular CellLight reagents are offered in small unit sizes (100 µL); all others are provided in a unit size of 1 mL. ‡ Also available is CellLight Nucleus-CFP (C10616) and CellLight Plasma Membrane-CFP (C10606), which generate a cyan-autofluorescent fusion protein targeted to the nucleus and plasma membrane, respectively. ** We also offer the CellLight Null (control) reagent (C10615), a negative transduction control for our CellLight targeted fluorescent reagents that comprises a suspension of baculovirus particles lacking mammalian genetic elements. 1. Curr Biol (1997) 7:176; 2. PLoS One (2009) 4:e8171; 3. J Cell Biol (1995) 130:639; 4. Plant J (2003) 33:775; 5. Curr Biol (1998) 8:377; 6. J Biol Chem (2004) 279:13044; 7. J Cell Sci (2005) 118:5243; 8. J Cell Biol (1989) 108:1657; 9. J Biol Chem (2009) 284:29218; 10. J Neurosci (2006) 26:3604; 11. FEBS Lett (1997) 405:18; 12. J Cell Biol (1998) 143:1505; 13. Trends Biochem Sci (1991) 16:478; 14. EMBO J (1997) 16:4983.
For Research Use Only. Not for use in diagnostic procedures.